Journal ArticleDOI
Strategy for far-field optical imaging and writing without diffraction limit
Reads0
Chats0
TLDR
In this article, a general concept for subdiffraction imaging and writing with focused light and conventional optics is described, which is unique in the fact that the giant optical nonlinearities required for breaking the diffraction barrier are realized at low light intensities.About:
This article is published in Physics Letters A.The article was published on 2004-05-31. It has received 175 citations till now. The article focuses on the topics: Near-field optics & Diffraction.read more
Citations
More filters
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
TL;DR: It is shown that synaptotagmin I, a protein resident in the vesicle membrane, remains clustered in isolated patches on the presynaptic membrane regardless of whether the nerve terminals are mildly active or intensely stimulated, suggesting that at least some vesicles constituents remain together during recycling.
Journal ArticleDOI
Microscopy and its focal switch
TL;DR: The principles of these methods together with their differences in implementation and operation are discussed, and potential developments are outlined.
Journal ArticleDOI
Three-dimensional optical laser lithography beyond the diffraction limit
TL;DR: In this article, a review of approaches aiming at translating this success in optical microscopy to optical lithography is presented, and basic principles and limitations, possible depletion mechanisms and recent lithography experiments by various groups are summarized.
Journal ArticleDOI
Nanoscale resolution in the focal plane of an optical microscope
Volker Westphal,Stefan W. Hell +1 more
TL;DR: It is demonstrated that en route to the molecular scale, the resolving power increases with the square root of the saturation level, which constitutes a new law regarding the resolution of an emerging class of far-field light microscopes that are not limited by diffraction.
References
More filters
Book
Principles of fluorescence spectroscopy
TL;DR: This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.
Journal ArticleDOI
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Stefan W. Hell,Jan Wichmann +1 more
TL;DR: A new type of scanning fluorescence microscope capable of resolving 35 nm in the far field is proposed, overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation point-spread function.
Journal ArticleDOI
Organic chemistry: a digital fluorescent molecular photoswitch.
TL;DR: This work uses external photostimulation to switch the fluorescence on and off from a single photochromic molecule embedded in a polymer film due to photo-isomerization of the molecule, which may eventually find application in erasable optical data-storage elements.
Journal ArticleDOI
Ground-state-depletion fluorscence microscopy: A concept for breaking the diffraction resolution limit
Stefan W. Hell,M. Kroug +1 more
TL;DR: In this paper, a novel concept in far-field fluorescence microscopy, ground state depletion (GSD), was introduced to overcome the classical diffraction resolution limit. But the concept was not applied to the localization of the outer region of the focus of a scanning fluorescence microscope.
Journal ArticleDOI
Saturated patterned excitation microscopy: a concept for optical resolution improvement
TL;DR: The theory of nonlinear patterned excitation microscopy is developed for achieving a substantial improvement in resolution by deliberate saturation of the fluorophore excited state and the effects of photon noise are included in the simulations.
Related Papers (5)
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Stefan W. Hell,Jan Wichmann +1 more