Journal ArticleDOI
Streptomyces rimosus extracellular proteases 3. Isolation and characterization of leucine aminopeptidase
TLDR
A leucine aminopeptidase was purified to homogeneity from Streptomyces rimosus culture filtrates, which are waste broth of oxytetracycline bioproduction process.Abstract:
A leucine aminopeptidase was purified to homogeneity fromStreptomyces rimosus culture filtrates, which are waste broth of oxytetracycline bioproduction process. Purification procedure includes ultrafiltration and chromatography on CM-Sephadex, AH-Sepharose and FPLC Mono S column.read more
Citations
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Journal ArticleDOI
Bacterial aminopeptidases: Properties and functions
TL;DR: The quoted studies demonstrate that bacterial aminopeptidases generally show Michaelis-Menten kinetics and can be placed into either of two categories based on their substrate specificity: broad or narrow.
Journal ArticleDOI
Properties of a novel D-stereospecific aminopeptidase from Ochrobactrum anthropi
TL;DR: Comparing the Km and Vmax values for the major substrates, it is clear that the enzyme prefers peptides to amino acid arylamides or amino acid amides.
Journal ArticleDOI
Induction of Mannanase, Xylanase, and Endoglucanase Activities in Sclerotium rolfsii
TL;DR: There is no common, coordinated regulatory mechanism that controls the synthesis of mannanase, xylanase, and endoglucanase in the plant-pathogenic basidiomycete Sclerotium rolfsii.
Journal ArticleDOI
Purification and Characterization of Exo-β-d-Glucosaminidase from a Cellulolytic Fungus,Trichoderma reesei PC-3-7
Masahiro Nogawa,Hiroya Takahashi,Aya Kashiwagi,Kenji Ohshima,Hirofumi Okada,Yasushi Morikawa +5 more
TL;DR: In this paper, an exo-β-d-glucosaminidase (Exoβd-N-acetylhexosamidase) was shown to be an endo-type chitosanase.
Journal ArticleDOI
Proteolytic enzymes from recombinant Streptomyces lividans TK24
TL;DR: Different proteases from the culture fluids of recombinant Streptomyces lividans strains were isolated and a chymotrypsin-chylike activity (CLA) was identified that specifically degrades a fusion protein between the alpha-amylase inhibitor from S. tendae and proinsulin from the monkey Macaca fascicularis.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
Book
Chromatographic and electrophoretic techniques
TL;DR: The reviewer was left with the impression that more attention is paid to these tests in the author's departments than is usually done in Great Britain.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. II. Head-related, aberrant tau-particles.
TL;DR: Temperature shift-down experiments, using several different phage carrying temperature-sensitive mutations in gene 21 , indicate that the bulk of the τ-particles cannot be used for normal phage production and strongly suggest that cleavage of the head proteins is required for DNA packaging to occur.
Journal ArticleDOI
Biological activities of leupeptins.
Takaaki Aoyagi,Setsuko Miyata,Masako Nanbo,Fukiko Kojima,Meiki Matsuzaki,Masaaki Ishizuka,Tomio Takeuchi,Hamao Umezawa +7 more
TL;DR: The effects of leupeptins on thrombokinase, thrombin, plasmin, trypsin, papain, kallikrein and α-chymotrypsin were compared with those of e-aminocaproic acid, trans-4-aminomethylcyclohexanecarboxylic acid, soybeantrypsin inhibitor and trasylol.