Structural determinants of concanavalin A specificity for oligosaccharides.
J U Baenziger,D Fiete +1 more
TLDR
Iodinated glycopeptides (50 to 200 x lo3 cpm/pmol) have been utilized to examine the structural determinants affecting interaction with the saccharide binding site of concanavalin A by Scatchard plot analyses of saturation curves.Citations
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Journal ArticleDOI
Carbohydrate-binding modules: fine-tuning polysaccharide recognition
TL;DR: The present review summarizes the impact structural biology has had on the understanding of the mechanisms by which CBMs bind to their target ligands.
Journal ArticleDOI
Specificity of Twelve Lectins Towards Oligosaccharides and Glycopeptides Related to N‐Glycosylproteins
TL;DR: The results obtained show that lectins considered 'identical' in terms of monosaccharide specificity, possess the ability to recognize fine differences in more complex structures, and different lectins are able to recognize different saccharidic sequences on the same glycan structure.
Book ChapterDOI
2 – Isolation, Physicochemical Characterization, and Carbohydrate-Binding Specificity of Lectins
Journal ArticleDOI
The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant.
TL;DR: Glycopeptide binding to lentil lectin-Sepharose was enhanced by the exposure of terminal N-acetylglucosamine residues on the glycopeptides, whereas binding to pea lectin -Sepharoses was enhancedBy the exposure to terminal mannose residues, and the differences in carbohydrate binding specificity were exploited to fractionate a mixture of [2-3H]mannose-labeled glycoproteins derived from mouse lymphoma cell gly
Journal ArticleDOI
Legume lectin structure.
TL;DR: The quaternary structures of these proteins form the basis of a higher level of specificity, where the spacing between individual epitopes of multivalent carbohydrates becomes important, and is of relevance for their effects on the biological activities of cells such as mitogenic responses.
References
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Journal ArticleDOI
The Attractions of Proteins for Small Molecules and Ions
TL;DR: The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story as discussed by the authors, and there are many compounds of serum albumin, which was used during the war by many chemists, most of whom found at least one 6ew compound.
Journal ArticleDOI
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay
A. E. Bolton,William M. Hunter +1 more
TL;DR: With some antisera the immunoreactivity of the antigen was diminished by the introduction of a single I atom into the tyrosyl groups, whereas antigen containing a single (125)I-labelled 3-(4-hydroxyphenyl)propionamide group showed the same immunore activity as the unmodified antigen.
Journal ArticleDOI
Protein-Carbohydrate Interaction. II. Inhibition Studies on the Interaction of Concanavalin A with Polysaccharides*
Journal ArticleDOI
Determination of aminosugar linkages in glycolipids by methylation: Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen☆
TL;DR: This method was successfully applied to analysis of aminosugar linkages in blood group B-active ceramide pentasaccharide from rabbit erythrocytes and in Forssman antigen of equine spleen.
Journal ArticleDOI
Evidence for receptor-mediated binding of glycoproteins, glycoconjugates, and lysosomal glycosidases by alveolar macrophages.
TL;DR: The results suggest the presence of a cell surface receptor on alveolar macrophages that binds glycoproteins having terminal sugars with the mannose or glucose configuration.