Journal ArticleDOI
Studies on interaction of anthracycline antibiotics and deoxyribonucleic acid: equilibrium binding studies on interaction of daunomycin with deoxyribonucleic acid.
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In this article, the interaction of daunomycin with DNA has been studied using equilibrium dialysis and fluorescence and absorbance titration, with an exclusion parameter of three to four base pairs.Abstract:
We have used equilibrium dialysis and fluorescence and absorbance titration to study the interaction of daunomycin with DNA. Our data at 200 mM Na+ are best fit by the neighbor exclusion model, with K = 7.0 x 10(5) M-1 and an exclusion parameter of three to four base pairs. The binding is dependent on ionic strength, with d log K/d log [Na+] = -0.84, from which we may estimate quantitatively ion release and the binding free energy corrected for the free energy of counterion release. From the temperature dependence of the binding constant, we find the binding to be exothermic, with a van't Hoff enthalpy of -12.8 kcal/mol. Competition dialysis experiments show that G+C base pairs are slightly preferred as binding sites for the drug and suggest that daunomycin binds preferentially to G+C pairs at low r. Cesium chloride density gradient sedimentation experiments provide an experimental demonstration of this preference. Daunomycin increases the Tm for DNA melting by some 30 degrees C as binding approaches saturation, with biphasic melting at low drug/base pair ratios. The data from these equilibrium studies are consistent with intercalative binding of daunomycin and provide a solid foundation for further structural and kinetic studies.read more
Citations
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Journal ArticleDOI
Neither delta- nor lambda-tris(phenanthroline)ruthenium(II) binds to DNA by classical intercalation.
TL;DR: Equilibrium binding studies and viscosity experiments are described that characterize the interaction of delta- and lambda-[Ru(o-phen)3]2+ with calf thymus DNA to show quantitatively that both the delta and lambda isomers are essentially electrostatically bound to DNA.
Journal ArticleDOI
Tris(phenanthroline)ruthenium(II) enantiomer interactions with DNA: Mode and specificity of binding
TL;DR: The results of these studies indicated that both isomers bind to DNA by a single mode, but the two isomers differ, however, in their effect on the hydrodynamic properties of DNA as measured by viscosity and, therefore, probably differ in their individual binding modes.
Journal ArticleDOI
DNA biosensors and microarrays.
TL;DR: This exhibition celebrates the centenary of the establishment of the University of Lyon with a celebration of the 100th anniversary of the birth of Jean-Bertrand Aristide Dejerine.
Journal ArticleDOI
Criteria for the mode of binding of DNA binding agents.
Dongchul Suh,Jonathan B. Chaires +1 more
TL;DR: Comparative fluorescence and hydrodynamic studies using the proven intercalator ethidium and the groove binder Hoechst 33258 demonstrate reliable criteria that may be used to distinguish intercalation from groove binding in the absence of high resolution structural data.
Journal ArticleDOI
DNA-binding and photocleavage studies of cobalt(III) polypyridyl complexes: [Co(phen)2IP]3+ and [Co(phen)2PIP]3+
TL;DR: In this article, two complexes of [Co(phen)2IP]3+ (IP=imidazo[4,5-f][1,10]phenanthroline) and [Co-(phen) 2PIP] 3+ (PIP=2-phenylimidazo [4, 5-f], 1,10]-phenanthropyline) have been synthesized and characterized by UV/VIS, IR, EA and mass spectra.
References
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The Attractions of Proteins for Small Molecules and Ions
TL;DR: The number and variety of known compounrjs between proteins and small molecules are increasing rapidly and make a fascinating story as discussed by the authors, and there are many compounds of serum albumin, which was used during the war by many chemists, most of whom found at least one 6ew compound.
Journal ArticleDOI
Theoretical aspects of DNA-protein interactions: co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous lattice.
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Journal ArticleDOI
Thermodynamic analysis of ion effects on the binding and conformational equilibria of proteins and nucleic acids: the roles of ion association or release, screening, and ion effects on water activity.
TL;DR: The purpose of this review is to examine the various effects of low- molecular-weight electrolytes on the associations and interactions of proteins and nucleic acids through general electrostatic effects rather than chemical effects of particular ions.
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Linked functions and reciprocal effects in hemoglobin: a second look.
TL;DR: In this article, the authors discuss linked functions and reciprocal effects in hemoglobin and show that not only do the linkage relations apply irrespective of whether the macromolecules undergo chemical change or polymerization, but also whether the ligands themselves associate and dissociate, possibly as macromocules.
Journal ArticleDOI
Base Specificity in the Interaction of Polynucleotides with Antibiotic Drugs
TL;DR: Chromomycin and olivomycin form complexes with DNA, preferably in the helical form, but not with RNA, and none of the other antibiotics requires the presence of any single base in the template for its action.