Journal ArticleDOI
Subpixel position measurement using 1D, 2D and 3D centroid algorithms with emphasis on applications in confocal microscopy
TLDR
In this article, the accuracy of centroid algorithms in 1D, 2D and 3D can be investigated and three factors that can influence the method's accuracy have been investigated: systematic error of the algorithm, influence of photon noise and the influence of perturbations such as scanning nonlinearity.Abstract:
The accuracy with which centroid algorithms in 1D, 2D and 3D can estimate an object's position has been investigated. Three factors that can influence the method's accuracy have been investigated: systematic error of the algorithm, influence of photon noise and the influence of perturbations such as scanning nonlinearity. The variation of the accuracy with parameters that are relevant for confocal microscopy, such as object diameter and photon noise/pixel, has been considered. Theory and simulations presented show that the variation of the accuracy with respect to such parameters can differ drastically between the 1D, 2D and 3D cases. Experiments performed using microspheres show that the magnitudes of the three types of error can be approximately the same under normal operating conditions and that it is therefore necessary to take all three into account when assessing the total error.read more
Citations
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Journal ArticleDOI
A review of progress in single particle tracking: from methods to biophysical insights.
TL;DR: The foundations of SPT are described together with novel optical implementations that nowadays allow the investigation of single molecule dynamic events with increasingly high spatiotemporal resolution using molecular densities closer to physiological expression levels.
Journal ArticleDOI
Quantitative Motion Analysis of Subchromosomal Foci in Living Cells Using Four-Dimensional Microscopy
TL;DR: The motion of subchromosomal foci and of whole chromosome territories in live human cell nuclei was investigated in four-dimensional space-time images and it was revealed that foci occasionally switched from random to directional motion inside chromosome territories.
Proceedings ArticleDOI
Empirical evaluation for finger input properties in multi-touch interaction
Feng Wang,Xiangshi Ren +1 more
TL;DR: This paper designed a series of experiments to explore human finger input properties and identified several useful properties such as contact area, contact shape and contact orientation which can be exploited to improve the performance of multi-touch selecting and pointing tasks.
Journal ArticleDOI
Fluctuations of the Red Blood Cell Membrane: Relation to Mechanical Properties and Lack of ATP Dependence
TL;DR: The membrane fluctuations of the red cell are governed by bending modulus, membrane tension, and cytosolic viscosity, with little or no dependence on the presence or absence of ATP.
Journal ArticleDOI
Three-dimensional spectral precision distance microscopy of chromatin nanostructures after triple-colour DNA labelling: a study of the BCR region on chromosome 22 and the Philadelphia chromosome.
Arif Esa,Peter Edelmann,Gregor Kreth,L. Trakhtenbrot,N. Amariglio,G. Rechavi,Michael Hausmann,Christoph Prof Dr Dr Cremer +7 more
TL;DR: In this article, spectral precision distance microscopy (SPDM) is used to estimate the bary centers (intensity weighted centroid analogous to the center of mass) of a given target in spectrally differential images.
References
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Journal ArticleDOI
Position measurement with a resolution and noise‐limited instrument
TL;DR: In this article, the authors derived theoretical limits on the ability to locate signal position by applying maximum likelihood estimation to this problem and showed that the limiting error in position measurement is a simple function of the instrument resolution, the density of sample points, and the signal-to-noise ratio of the data.
Journal ArticleDOI
Elimination of systematic error in subpixel accuracy centroid estimation [also Letter 34(11)3347-3348(Nov1995)]
Brian F. Alexander,Kim Chew Ng +1 more
TL;DR: Both simulation and experimental tests of the centroid method for subpixel accuracy image feature location prediction are presented for the case of the location of the center lines of the images of projected light stripes in a triangulation-based three-dimensional shape measurement system.
Journal ArticleDOI
The influence of specimen refractive index, detector signal integration, and non-uniform scan speed on the imaging properties in confocal microscopy
TL;DR: In this paper, the influence of refractive index on the depth resolution and geometry of confocal microscopy images was investigated for a uniform specimen volume and it was found that depth resolution is more severely affected than the lateral resolution.
Journal ArticleDOI
Centroid position measurements and subpixel sensitivity variations with the MAMA detector
TL;DR: Initial measurements of the position sensitivity of a visible-light multi-anode microchannel array (MAMA) detector show that centroid calculations for image spots are accurate to better than 0.04 pixels even with sources that are essentially delta functions at the photocathode.
Proceedings ArticleDOI
A survey and examination of subpixel measurement techniques
Geoff West,T. A. Clarke +1 more
TL;DR: This paper reviews proposed subpixel methods in the context of an increasingly important application, namely, the determination of the position of a laser spot on a sensing array for triangulation.