Surface plasmon resonance analysis at a supported lipid monolayer
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TLDR
Conditions for the formation of lipid monolayers have been optimised with respect to lipid type, chemical and buffer compatibility, ligand stability and reproducibility.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1998-08-14 and is currently open access. It has received 123 citations till now. The article focuses on the topics: Surface plasmon resonance & Monolayer.read more
Citations
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Journal ArticleDOI
Surface Plasmon Resonance: A Versatile Technique for Biosensor Applications
TL;DR: A wide range of applications in optical-based sensors using either surface plasmon resonance (SPR) or surface plAsmon resonance imaging (SPRI) are discussed, with examples from the biomedical, proteomics, genomics and bioengineering fields.
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Amyloidogenic Protein–Membrane Interactions: Mechanistic Insight from Model Systems
TL;DR: Recent studies with artificial model membranes have highlighted the striking resemblance of the mechanisms of membrane permeabilization of amyloid-forming proteins to those of pore-forming toxins and antimicrobial peptides.
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Protein adsorption on supported phospholipid bilayers.
TL;DR: Quartz crystal microbalance with dissipation measurements were used to investigate the adsorption of human fibrinogen, human serum albumin, bovine hemoglobin, horse heart cytochrome c, human immunoglobulin, and 10% fetal bovines serum on supported bilayers of egg-phosphatidylcholine (eggPC) lipids.
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Surface plasmon resonance in protein-membrane interactions
TL;DR: A brief overview of different membrane-mimetic surfaces that can be prepared on the surface of SPR chips, properties of liposomes on thesurface of L1 chips and some selected examples of protein-membrane interactions studied with such system are given.
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Advances in membrane receptor screening and analysis.
TL;DR: This review focuses on developments using label‐free assays that facilitate ligand–membrane–receptor screening without the need for chemical‐, biological‐ or radiological‐labelled reagents.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Small-volume extrusion apparatus for preparation of large, unilamellar vesicles
Robert C. MacDonald,Ruby I. MacDonald,Bert Ph. M. Menco,Keizo Takeshita,Nanda K. Subbarao,Lan-rong Hu +5 more
TL;DR: The filter holder provides for back-and-forth passage of the sample between two syringes, a design that minimizes filter blockage, eliminates the need to change filters during LUVET preparation and reduces preparation time to a few minutes.
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Quantitative determination of surface concentration of protein with surface plasmon resonance using radiolabeled proteins
TL;DR: In this article, a methodology to correlate the absolute surface concentration of protein to the surface plasmon resonance (SPR) response is described, and the thickness and the optical constants for each layer on the sensor chip used were determined with different optical techniques.
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Lipid bilayer thickness varies linearly with acyl chain length in fluid phosphatidylcholine vesicles
TL;DR: The thickness of the lipid bilayer in vesicles made of pure phosphatidylcholines, with acyl chain lengths ranging from 10 to 24 carbons, has been determined by analysis of continuous X-ray scattering data from vesicle pellets at temperatures above the lipid phase transition temperature.
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Determination of rate and equilibrium binding constants for macromolecular interactions using surface plasmon resonance: use of nonlinear least squares analysis methods.
TL;DR: It is demonstrated that, at least for the interaction investigated, the apparent rate and equilibrium binding constants determined using SPR are concentration independent and can be determined with good reproducibility.