T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1
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TLDR
It is shown that the CD28 antigen, expressed in Chinese hamster ovary cells, mediated specific intercellular adhesion with human lymphoblastoid and leukemic B-cell lines and with activated primary murine B cells, and represents a heterophilic interaction between members of the immunoglobulin superfamily that may serve to regulate T-cell cytokine levels at sites of B- cell activation.Abstract:
Studies using monoclonal antibodies (mAbs) have implicated the homodimeric glycoprotein CD28 as an important regulator of human T-cell activation, in part by posttranscriptional control of cytokine mRNA levels. Although the CD28 antigen has functional and structural characteristics of a receptor, a natural ligand for this molecule has not been identified. Here we show that the CD28 antigen, expressed in Chinese hamster ovary (CHO) cells, mediated specific intercellular adhesion with human lymphoblastoid and leukemic B-cell lines and with activated primary murine B cells. CD28-mediated adhesion was not dependent upon divalent cations. Several mAbs were identified that inhibited CD28-mediated adhesion, including mAb BB-1 against the B-cell activation antigen B7/BB-1 and some mAbs against major histocompatibility complex class I antigens. B7/BB-1 expression correlated closely with CD28-mediated adhesion, but class I expression did not. Transfected COS cells expressing the B7/BB-1 antigen adhered to CD28+ CHO cells; this adhesion was blocked by mAbs to CD28 and B7/BB-1. The specific recognition by CD28 of the B-cell activation antigen B7/BB-1 represents a heterophilic interaction between members of the immunoglobulin superfamily that may serve to regulate T-cell cytokine levels at sites of B-cell activation.read more
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The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity
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Cd28/b7 system of t cell costimulation
TL;DR: This review summarizes the state of CD28/B7 immunobiology both in vitro and in vivo; summarizes the many experiments that have led to the current understanding of the participants in this complex receptor/ligand system; and illustrates the current models for CD28-mediated T cell and B cell regulation.
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Molecular mechanisms of T cell co-stimulation and co-inhibition
TL;DR: The mechanisms through which T cell activation, differentiation and function is controlled by co-stimulatory and co-inhibitory receptors are reviewed.
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CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation.
TL;DR: It is shown here that the presence of low levels of B7-2 on freshly explanted T cells can partially inhibit T cell proliferation, and this inhibition is mediated by interactions with CTLA-4, which strongly suggests that the outcome of T cell antigen receptor stimulation is regulated by CD28 costimulatory signals, as well as inhibitory signals derived from CTla-4.
References
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Journal ArticleDOI
Interaction between CD4 and class II MHC molecules mediates cell adhesion
Carolyn Doyle,Jack L. Strominger +1 more
TL;DR: The CD4 protein, even in the absence of T-cell receptor-antigen interactions, can interact directly with class II antigens to function as a cell surface adhesion molecule.