Journal ArticleDOI
Tailored Biosynthesis of Olefinic Medium-Chain-Length Poly[(R)-3-hydroxyalkanoates] in Pseudomonas putida GPo1 with Improved Thermal Properties
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In this paper, a chemostat culture of Pseudomonas putida GPo1 under well-defined dual-(C,N)-nutrient limited growth conditions was used to produce tailor-made copolymers.Abstract:
Mixtures of 5-phenylvalerate, octanoate, and 10-undecenoate were fed to a chemostat culture (dilution rate = 0.1 h-1) of Pseudomonas putida GPo1 under well-defined dual-(C,N)-nutrient limited growth conditions. Five new, tailor-made copolymers were produced and consisted of poly(3-hydroxy-5-phenylvalerate-co-3-hydroxyalkanoates-co-3-hydroxy-ω-alkenoates), poly(HP-co-HA-co-HE), with increasing amounts of aromatic side chains (A, 0%; B, 3%; C, 19%; D, 42%; and E, 59%), approximately 10 mol % unsaturated side chains, and decreasing amounts of saturated side chains. On the basis of NMR analysis of polymer E, it was concluded that the incorporation of the substrates occurred randomly. The HP-content determined the glass transition temperature, which increased linearly from −38.7 °C for poly(0%HP-co-90%HA-co-10%HE) to −6.0 °C for poly(59%HP-co-31%HA-co-10%HE).read more
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Journal ArticleDOI
Producing microbial polyhydroxyalkanoate (PHA) biopolyesters in a sustainable manner
TL;DR: Emphasis is devoted to the integration of PHA-production based on selected raw materials into the holistic patterns of sustainability; this encompasses the choice of new, powerful microbial production strains, non-hazardous, environmentally benign methods for PHA recovery, and reutilization of waste streams from the PHA production process itself.
Journal ArticleDOI
Start a research on biopolymer polyhydroxyalkanoate (PHA) : a review
Giin-Yu Amy Tan,Chia-Lung Chen,Ling Li,Liya Ge,Lin Wang,Indah Mutiara Ningtyas Razaad,Yanhong Li,Lei Zhao,Yu Mo,Jing-Yuan Wang +9 more
TL;DR: This review aims to facilitate the start-up of PHA research by providing a summary of commercially available PHA-accumulating microbial cultures, PHA biosynthetic pathways, and methods for PHA detection, extraction and analysis.
Journal ArticleDOI
Increased diversification of polyhydroxyalkanoates by modification reactions for industrial and medical applications
Baki Hazer,Alexander Steinbüchel +1 more
TL;DR: The large variability of PHAs regarding chemical structure and material properties that can be currently produced is summarized.
Journal ArticleDOI
In-silico-driven metabolic engineering of Pseudomonas putida for enhanced production of poly-hydroxyalkanoates.
Ignacio Poblete-Castro,Danielle Binger,André Luis Rodrigues,Judith Becker,Vitor A. P. Martins dos Santos,Christoph Wittmann +5 more
TL;DR: The mutant P. putida Δgcd, generated on basis of the computational design, exhibited 100% increased PHA accumulation as compared to the parent wild type, maintained a high specific growth rate and exhibited an almost unaffected gene expression profile, which excluded detrimental side effects of the modification.
Journal Article
Modern Biotechnological Polymer Synthesis: A Review
TL;DR: In this article, the authors provided an insight into recent advances in allocation, pretreatment and utilization of raw materials available for biopolymer production in different areas of the world and elucidated the high potential and risks of applying continuous process conduction in comparison with batch and fed batch fermentation mode.