Testosterone induces an intracellular calcium increase by a nongenomic mechanism in cultured rat cardiac myocytes.
Jose Miguel Vicencio,Cristian Ibarra,Manuel Estrada,Mario Chiong,Dagoberto Soto,Valentina Parra,Guillermo Díaz-Araya,Enrique Jaimovich,Sergio Lavandero +8 more
TLDR
The mechanism for the rapid, testosterone-induced increase in intracellular Ca2+ is through activation of a plasma membrane receptor associated with a Pertussis toxin-sensitive G protein-phospholipase C/inositol 1,4,5-trisphosphate signaling pathway.Abstract:
Androgens are associated with important effects on the heart, such as hypertrophy or apoptosis. These responses involve the intracellular androgen receptor. However, the mechanisms of how androgens activate several membrane signaling pathways are not fully elucidated. We have investigated the effect of testosterone on intracellular calcium in cultured rat cardiac myocytes. Using fluo3-AM and epifluorescence microscopy, we found that exposure to testosterone rapidly (1–7 min) led to an increase of intracellular Ca2+, an effect that persisted in the absence of external Ca2+. Immunocytochemical analysis showed that these effects occurred before translocation of the intracellular androgen receptor to the perinuclear zone. Pretreatment of the cells with 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid-acetoxymethylester and thapsigargin blocked this response, suggesting the involvement of internal Ca2+ stores. U-73122, an inhibitor of phospholipase C, and xestospongin C, an inhibitor of inositol 1,4,5-...read more
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Non-genomic Actions of Androgens
TL;DR: The non-genomic effects of androgens are reviewed, along with a discussion of the possible role non- genomic androgen actions have on animal physiology and behavior.
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Mechanistic Pathways of Sex Differences in Cardiovascular Disease
TL;DR: There is a need for a more detailed understanding of sex differences and their underlying mechanisms, which holds the potential to design new drugs that target sex-specific cardiovascular mechanisms and affect phenotypes.
Androgen Receptor Structure, Function and Biology: From Bench to Bedside.
Rachel A Davey,Mathis Grossmann +1 more
TL;DR: An overview of the structure, function, signalling pathways and biology of the AR as well as its important role in clinical medicine, with emphasis on recent developments in this field is presented.
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Faisal Rahman,Helen C. Christian +1 more
TL;DR: Evidence for rapid testosterone actions, which have clinical implications in fertility, cardiovascular disease and the treatment of prostate cancer are discussed.
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Rapid actions of androgens.
Guido Michels,Uta C. Hoppe +1 more
TL;DR: This review will focus on the rapid effects of androgen on cell surface and cytoplasmic level, which suggests a cross-talk between the fast non-genomic and the slow genomic pathway of androgens.
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TL;DR: AIS is an archetypal example of a hormone resistance disorder; however, due to defective androgen receptor (AR1) function, there is loss of target organ response to the hormone, and the effects of androgens are reduced or absent.
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Shalender Bhasin,Linda J. Woodhouse,Richard Casaburi,Atam B. Singh,Dimple Bhasin,Nancy Berman,Xianghong Chen,Kevin E. Yarasheski,Lynne Magliano,Connie Dzekov,Jeanne Dzekov,Rachelle Bross,Jeff M. Phillips,Indrani Sinha-Hikim,Ruoquing Shen,Thomas W. Storer +15 more
TL;DR: It is concluded that changes in circulating testosterone concentrations, induced by GnRH agonist and testosterone administration, are associated with testosterone dose- and concentration-dependent changes in fat-free mass, muscle size, strength and power, fat mass, hemoglobin, HDL cholesterol, and IGF-I levels, in conformity with a single linear dose-response relationship.
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Specific, nongenomic actions of steroid hormones
TL;DR: Mechanisms of action are being studied with regard to signal perception and transduction, and researchers have developed a patchy sketch of a membrane receptor-second messenger cascade similar to those involved in catecholamine and peptide hormone action.