Journal ArticleDOI
The cell biology of major histocompatibility complex class I assembly: towards a molecular understanding.
A. Van Hateren,Eddie A. James,Alistair Bailey,Andrew Phillips,Andrew Phillips,Neil Dalchau,Neil Dalchau,Tim Elliott +7 more
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TLDR
This work discusses how the aminopeptidase ERAAP fine-tunes the peptide repertoire available to assembling MHC I molecules, before focusing on the journey of MHCI molecules through the secretory pathway, where calreticulin provides additional regulation of M HC I expression.Abstract:
Major histocompatibility complex class I (MHC I) proteins protect the host from intracellular pathogens and cellular abnormalities through the binding of peptide fragments derived primarily from intracellular proteins. These peptide-MHC complexes are displayed at the cell surface for inspection by cytotoxic T lymphocytes. Here we reveal how MHC I molecules achieve this feat in the face of numerous levels of quality control. Among these is the chaperone tapasin, which governs peptide selection in the endoplasmic reticulum as part of the peptide-loading complex, and we propose key amino acid interactions central to the peptide selection mechanism. We discuss how the aminopeptidase ERAAP fine-tunes the peptide repertoire available to assembling MHC I molecules, before focusing on the journey of MHC I molecules through the secretory pathway, where calreticulin provides additional regulation of MHC I expression. Lastly we discuss how these processes culminate to influence immune responses.read more
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Major Histocompatibility Complex (MHC) Class I and MHC Class II Proteins: Conformational Plasticity in Antigen Presentation.
Marek Wieczorek,Esam T. Abualrous,Jana Sticht,Miguel Álvaro-Benito,Sebastian Stolzenberg,Frank Noé,Christian Freund +6 more
TL;DR: Recent studies predict a high impact of protein intermediate states on MHC allele-specific peptide presentation, which implies a profound influence of MHC dynamics on the phenomenon of immunodominance and the development of autoimmune diseases.
Journal ArticleDOI
Antigen processing and immune regulation in the response to tumours.
TL;DR: Modifications to the antigen processing and presentation pathway in tumours are reviewed and how it affects the anti‐tumour immune response is reviewed, considering the role of tumour‐infiltrating cell populations and highlighting possible future therapeutic targets.
Journal ArticleDOI
Distinguishing functional polymorphism from random variation in the sequences of >10,000 HLA-A, -B and -C alleles
James Robinson,James Robinson,Lisbeth A. Guethlein,Nezih Cereb,Soo Young Yang,Paul Norman,Steven G.E. Marsh,Steven G.E. Marsh,Peter Parham +8 more
TL;DR: Most alleles of HLA class I (>80%) are very rare, often identified in one person or family, and they differ by point mutation from older, more common alleles, reflect the germ-line mutation rate.
Journal ArticleDOI
Regulation of the Cell Biology of Antigen Cross-Presentation
TL;DR: Three distinct pathways of vesicular traffic converge to form the ideal cross- presentation compartment, each regulated differently to supply a unique component that enables cross-presentation of a diverse repertoire of peptides.
Journal ArticleDOI
Tapasin-related protein TAPBPR is an additional component of the MHC class I presentation pathway
Louise H. Boyle,Clemens Hermann,Jessica M. Boname,Keith M. Porter,Peysh A. Patel,Marian L. Burr,Lidia M. Duncan,Michael E. Harbour,David Rhodes,Karsten Skjødt,Paul J. Lehner,John Trowsdale +11 more
TL;DR: The identification of TAPBPR as an additional component of the M HC class I antigen-presentation pathway demonstrates that mechanisms controlling MHC class I expression remain incompletely understood.
References
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UCSF Chimera--a visualization system for exploratory research and analysis.
Eric F. Pettersen,Thomas D. Goddard,Conrad C. Huang,Gregory S. Couch,Daniel M. Greenblatt,Elaine C. Meng,Thomas E. Ferrin +6 more
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Comparative Protein Structure Modeling Using MODELLER
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TL;DR: This unit describes how to calculate comparative models using the program MODELLER and discusses all four steps of comparative modeling, frequently observed errors, and some applications.
Journal ArticleDOI
Comparative protein structure modeling using Modeller.
Narayanan Eswar,Ben Webb,Marc A. Marti-Renom,Mallur S. Madhusudhan,David Eramian,Min-Yi Shen,Ursula Pieper,Andrej Sali +7 more
TL;DR: This unit describes how to calculate comparative models using the program MODELLER and discusses all four steps of comparative modeling, frequently observed errors, and some applications.
Journal ArticleDOI
HADDOCK: a protein-protein docking approach based on biochemical or biophysical information.
TL;DR: An approach called HADDOCK (High Ambiguity Driven protein-protein Docking) that makes use of biochemical and/or biophysical interaction data such as chemical shift perturbation data resulting from NMR titration experiments or mutagenesis data to drive the docking process.
Journal ArticleDOI
SYFPEITHI: database for MHC ligands and peptide motifs.
TL;DR: The first version of the major histocompatibility complex (MHC) databank SYFPEITHI: database for MHC ligands and peptide motifs, is now available to the general public.