Journal ArticleDOI
The complete amino acid sequence of a major wheat protein inhibitor of α-amylase
Nizar Kashlan,Michael Richardson +1 more
TLDR
The complete amino acid sequence of one of the major wheat protein iso-inhibitors of α-amylase was determined from analysis of fragments and peptides derived from the protein by cleavage with cyanogen bromide and by digestion with trypsin, chymotrypsin , thermolysin and the Staphylococcus aureus V8 protease.About:
This article is published in Phytochemistry.The article was published on 1981-01-01. It has received 87 citations till now. The article focuses on the topics: Peptide sequence & Chymotrypsin.read more
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Protease Inhibitors in Plants: Genes for Improving Defenses Against Insects and Pathogens
TL;DR: The inducible chemicals, which can also occur constitutively, include such complex sub stances as antibiotics, alkaloids, and terpenes, as well as proteins such as enzymes, enzyme inhibitors, and lectins, which have provided interesting systems for studying inducibles plant defense.
Journal ArticleDOI
Plant alpha-amylase inhibitors and their interaction with insect alpha-amylases.
TL;DR: In this review, existing knowledge of insect α-amylases, plant α-AMylase inhibitors and their interaction is summarized and positive results recently obtained for transgenic plants and future prospects in the area are reviewed.
Journal ArticleDOI
Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and developmental gene expression.
TL;DR: The identity of puroindolines to wheat starch-granule associated proteins is discussed as well as the potential role of pocindoline in the plant defence mechanism.
Journal ArticleDOI
Molecular evolution of the seed storage proteins of barley, rye and wheat.
TL;DR: It is shown that parts of the non-repetitive domain of one group of prolamins are homologous with sequences present in a large group of seed proteins from monocotyledonous and dicotylingonous plants; including Bowman-Birk protease inhibitors, cereal inhibitors of alpha-amylase and trypsin, and 2 S globulin storage proteins of castor bean and oil seed rape.
Journal ArticleDOI
Transgenic potato plants with enhanced resistance to the tomato moth, Lacanobia oleracea: growth room trials
Angharad M. R. Gatehouse,Gillian M. Davison,Christine A. Newell,Andrew Merryweather,William D. O. Hamilton,Elisabeth P. J. Burgess,Robert J.C. Gilbert,John A. Gatehouse +7 more
TL;DR: The hypothesis that GNA has a significant antifeedant effect on insects is supported, and expression of CpTI in transgenic potatoes had similar effects to expression of GNA on total insect biomass and survival, but did not afford protection against insect damage to the plant.
References
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Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
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The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins.
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Micro-sequence analysis of peptides and proteins using 4-NN-dimethylaminoazobenzene 4′-isothiocyanate/phenylisothiocyanate double coupling method
TL;DR: The pheny~sot~ocyanate (Edman) degradation has been a most valuable technique in amino acid sequence determination and contributed, to a great extent, to the sequence analysis of proteins which are available in only limited quantities.
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Anomalous cleavage of aspartyl-proline peptide bonds during amino acid sequence determinations.
TL;DR: The enhanced rate with proline as compared to other amino acids is undoubtedly due to the greater basicity of the proline nitrogen.
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Wheat protein inhibitors of α-amylase
TL;DR: The location in the seed, molecular properties and biological role of protein α-amylase inhibitors from wheat are discussed, and genetic aspects of the albumin inhibitor production and the possibility of improving nutritional value and insect resistance are described.