Journal ArticleDOI
The IS4 family of insertion sequences : evidence for a conserved transposase motif
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TLDR
Comparison with all the proka‐ryotic transposable elements sequenced so far revealed that the IS231 transposases share two conserved regions with those of 35 other insertion sequences of wide origins.Abstract:
The eight IS231 variants characterized so far (IS231 A-F, V and W) display similar transposases with an overall 40% identity. Comparison with all the prokaryotic transposable elements sequenced so far revealed that the IS231 transposases share two conserved regions with those of 35 other insertion sequences of wide origins. These insertion sequences, defining the IS4 family, have a common bipartite organization of their ends and are divided into two similarity groups. Interestingly, the transposase domains conserved within this family display similarities with the well known integrase domain shared by transposases of the IS3 and IS15 families, and integrases of retroelements. This domain is also found in IS30-related elements and Tn7 TnsB protein. Amino acid residues conserved throughout all these prokaryotic and eukaryotic mobile genetic elements define a major transposase/integrase motif, likely to play an important role in the transposition process.read more
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Journal ArticleDOI
Bacillus thuringiensis and Its Pesticidal Crystal Proteins
E. Schnepf,Neil Crickmore,J. Van Rie,Didier Lereclus,J. Baum,Jerald S. Feitelson,Daniel R. Zeigler,Donald H. Dean +7 more
TL;DR: Researchers are reporting promising results in engineering more-useful toxins and formulations, in creating transgenic plants that express pesticidal activity, and in constructing integrated management strategies to insure that these products are utilized with maximum efficiency and benefit.
Journal ArticleDOI
Bacterial insertion sequences: their genomic impact and diversity
TL;DR: This review focuses on prokaryotic ISs, and explains how ISs are identified and classified into families by a combination of characteristics including their transposases (Tpases), their overall genetic organisation and the accessory genes which some ISs carry.
Journal ArticleDOI
An active DNA transposon family in rice
Ning Jiang,Zhirong Bao,Xiaoyu Zhang,Hirohiko Hirochika,Sean R. Eddy,Susan R. McCouch,Susan R. Wessler +6 more
TL;DR: The use of draft sequences for the two subspecies of Oryza sativa, Nipponbare and indica, provides a unique opportunity to study the dynamics of transposable elements in this important crop plant and is used in a computational approach to identify the first active DNA transposons from rice and the firstactive miniature inverted-repeat transPOSable element (MITE) from any organism.
Journal ArticleDOI
Three-dimensional structure of the Tn5 synaptic complex transposition intermediate.
TL;DR: The three-dimensional structure of prokaryotic Tn5 transposase complexed with Tn 5 transposon end DNA determined to 2.3 angstrom resolution is reported, which provides a molecular framework for understanding many aspects of transposition, including the binding of transpos on end DNA by one subunit and cleavage by a second.
Journal ArticleDOI
Sequence and Organization of pXO1, the Large Bacillus anthracis Plasmid Harboring the Anthrax Toxin Genes
Richard T. Okinaka,K. Cloud,Oliver A. Hampton,Alex R. Hoffmaster,Karen K. Hill,Paul Keim,Theresa M. Koehler,G. Lamke,Satoshi Kumano,Jacques Mahillon,Daniel K. Manter,Y. Martinez,Darrell O. Ricke,Rita Svensson,Paul J. Jackson +14 more
TL;DR: Among the ORFs with a high degree of similarity to known sequences are a collection of putative transposases, resolvases, and integrases, suggesting an evolution involving lateral movement of DNA among species.
References
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Rapid and sensitive protein similarity searches
TL;DR: An algorithm was developed which facilitates the search for similarities between newly determined amino acid sequences and sequences already available in databases and increases sensitivity by giving high scores to those amino acid replacements which occur frequently in evolution.
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HIV-1 DNA integration: Mechanism of viral DNA cleavage and DNA strand transfer
TL;DR: Results suggest that both reactions occur by a one-step mechanism without involvement of a covalent protein-DNA intermediate in HIV-1 integration.
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Signals for ribosomal frameshifting in the Rous sarcoma virus gag-pol region.
TL;DR: A short sequence of RSV RNA, 147 nucleotides in length, containing the frameshift site and stem-loop structure, is sufficient to direct frameshifting in a novel genetic context.
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Nucleotide sequence of the kanamycin resistance transposon Tn903.
TL;DR: It is suggested that Tn903 could possibly code for at least three high molecular weight polypeptides, one in the region between the two 1057 base-pair sequences is suggested to be the kanamycin resistance determinant (aminoglycoside 3′-phosphotransferase) from its location and size.
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Residues critical for retroviral integrative recombination in a region that is highly conserved among retroviral/retrotransposon integrases and bacterial insertion sequence transposases.
TL;DR: Comparison of deduced amino acid sequences for retroviral/retrotransposon integrase (IN) proteins of several organisms reveals strong conservation of a constellation of amino acids characterized by two invariant aspartate (D) residues and a glutamate (E) residue, which is referred to as the D,D(35)E region.