Journal ArticleDOI
The pathway of neutrophil extracellular traps towards atherosclerosis and thrombosis
Reads0
Chats0
TLDR
It has been demonstrated that NETs are present in atherosclerotic lesions of both humans and animal models and are implicated in various mechanisms leading to atherogenesis, and NETs promote the accumulation of prothrombotic molecules, thus significantly contributing to thrombus formation.About:
This article is published in Atherosclerosis.The article was published on 2019-09-01. It has received 89 citations till now. The article focuses on the topics: Neutrophil extracellular traps.read more
Citations
More filters
Journal ArticleDOI
Neutrophil Extracellular Traps Participate in Cardiovascular Diseases: Recent Experimental and Clinical Insights.
TL;DR: This review presents current concepts of neutrophil biology, the triggers to and mechanisms of NET formation, and the contribution ofNETs to atherosclerosis and to thrombosis, and considers the use of markers of NETs in clinical studies.
Journal ArticleDOI
NETosis: Molecular Mechanisms, Role in Physiology and Pathology.
N.V. Vorobjeva,Boris V. Chernyak +1 more
TL;DR: Basic mechanisms of NETosis, as well as its role in the pathogenesis of some diseases including COVID-19 are discussed.
Journal ArticleDOI
Insights Into Immunothrombosis: The Interplay Among Neutrophil Extracellular Trap, von Willebrand Factor, and ADAMTS13.
Junxian Yang,Junxian Yang,Zhiwei Wu,Zhiwei Wu,Quan Long,Jiaqi Huang,Tiantian Hong,Tiantian Hong,Wang Liu,Jiangguo Lin,Jiangguo Lin +10 more
TL;DR: Targeting the NET-VWF axis may be a novel therapeutic strategy for inflammation-associated TMAs, AIS, and COVID-19.
Journal ArticleDOI
Metformin, Macrophage Dysfunction and Atherosclerosis.
TL;DR: In this article, the authors discussed the future research directions of metformin: single-cell RNA sequencing, neutrophil extracellular traps (NETs), epigenetic modification, and metin-based combination drugs.
Journal ArticleDOI
Immune complexes, innate immunity, and NETosis in ChAdOx1 vaccine-induced thrombocytopenia.
Sverre Holm,Hassen Kared,Hassen Kared,Annika E. Michelsen,Annika E. Michelsen,Xiang Yi Kong,Tuva B. Dahl,Nina Haagenrud Schultz,Nina Haagenrud Schultz,Tuula A. Nyman,Tuula A. Nyman,Cathrine Fladeby,Ingebjørg Seljeflot,Ingebjørg Seljeflot,Thor Ueland,Thor Ueland,Thor Ueland,Maria Stensland,Maria Stensland,Siri Mjaaland,Guro Løvik Goll,Lise Sofie H. Nissen-Meyer,Pål Aukrust,Pål Aukrust,Karolina Skagen,Ida Gregersen,Mona Skjelland,Mona Skjelland,Pål Andre Holme,Pål Andre Holme,Ludvig A. Munthe,Ludvig A. Munthe,Bente Halvorsen,Bente Halvorsen +33 more
Abstract: Aims We recently reported five cases of vaccine-induced immune thrombotic thrombocytopenia (VITT) 7-10 days after receiving the first dose of the ChAdOx1 nCoV-19 adenoviral vector vaccine against corona virus disease 2019 (COVID-19). We aimed to investigate the pathogenic immunological responses operating in these patients. Methods and results We assessed circulating inflammatory markers by immune assays and immune cell phenotyping by flow cytometry analyses and performed immunoprecipitation with anti-platelet factor (PF)4 antibody in plasma samples followed by mass spectrometry from all five patients. A thrombus was retrieved from the sinus sagittal superior of one patient and analysed by immunohistochemistry and flow cytometry. Precipitated immune complexes revealed multiple innate immune pathway triggers for platelet and leucocyte activation. Plasma contained increased levels of innate immune response cytokines and markers of systemic inflammation, extensive degranulation of neutrophils, and tissue and endothelial damage. Blood analyses showed activation of neutrophils and increased levels of circulating H3Cit, dsDNA, and myeloperoxidase-DNA complex. The thrombus had extensive infiltration of neutrophils, formation of neutrophil extracellular traps (NETs), and IgG deposits. Conclusions The results show that anti-PF4/polyanion IgG-mediated thrombus formation in VITT patients is accompanied by a massive innate immune activation and particularly the fulminant activation of neutrophils including NETosis. These results provide novel data on the immune response in this rare adenoviral vector-induced VITT.
References
More filters
Journal ArticleDOI
Neutrophil extracellular traps kill bacteria
Volker Brinkmann,Ulrike Reichard,Christian Goosmann,Beatrix Fauler,Yvonne Uhlemann,David S. Weiss,Yvette Weinrauch,Yvette Weinrauch,Arturo Zychlinsky +8 more
TL;DR: It is described that, upon activation, neutrophils release granule proteins and chromatin that together form extracellular fibers that bind Gram-positive and -negative bacteria, which degrade virulence factors and kill bacteria.
Journal ArticleDOI
Novel cell death program leads to neutrophil extracellular traps
Tobias A. Fuchs,Ulrike Abu Abed,Christian Goosmann,Robert Hurwitz,Ilka Schulze,Volker Wahn,Yvette Weinrauch,Volker Brinkmann,Arturo Zychlinsky +8 more
TL;DR: This novel ROS-dependent death allows neutrophils to fulfill their antimicrobial function, even beyond their lifespan.
Journal ArticleDOI
Platelet TLR4 activates neutrophil extracellular traps to ensnare bacteria in septic blood
Stephen Clark,Adrienne C. Ma,Samantha A. Tavener,Braedon McDonald,Zahra Goodarzi,Margaret M. Kelly,Kamala D. Patel,Subhadeep Chakrabarti,Erin F. McAvoy,Gary D. Sinclair,Elizabeth Keys,Emma Allen-Vercoe,Rebekah DeVinney,Christopher J. Doig,Francis H. Y. Green,Paul Kubes +15 more
TL;DR: It is proposed that platelet TLR4 is a threshold switch for this new bacterial trapping mechanism in severe sepsis, where NETs have the greatest capacity for bacterial trapping.
Journal ArticleDOI
Extracellular DNA traps promote thrombosis
Tobias A. Fuchs,Alexander Brill,Daniel Duerschmied,Daphne Schatzberg,Marc Monestier,Daniel D. Myers,Shirley K. Wrobleski,Thomas W. Wakefield,John H. Hartwig,Denisa D. Wagner +9 more
TL;DR: It is reported that NETs provide a heretofore unrecognized scaffold and stimulus for thrombus formation and may further explain the epidemiological association of infection with thrombosis.
Journal ArticleDOI
Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps
TL;DR: Neutrophil elastase escapes azurophilic granules, translocates to the nucleus, and degrades histones to promote chromatin decondensation necessary for NET formation.