The relationship between in vitro cellular aging and in vivo human age.
Edward L. Schneider,Youji Mitsui +1 more
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TLDR
Although early and late passage cultures of human diploid cells may provide an important cell system for examining loss of replicative potential, fibroblast cultures derived from old and young human donors may be a more appropriate model system for studying human cellular aging.Abstract:
Differences between early and late passage cell cultures on the organelle and macromolecular levels have been attributed to cellular "aging". However, concern has been expressed over whether changes in diploid cell populations after serial passage in vitro accurately reflect human cellular aging in vivo. Studies were therefore undertaken to determine if significant differences would be observed in the in vitro lifespans of skin fibroblast cultures from old and young normal, non-hospitalized volunteers and to examine if parameters that change with in vitro "aging" are altered as a function of age in vivo. Statistically signigificant (P less than 0.05) decreases were found in the rate of fibroblast migration, onset of cell culture senescence, in vitro lifespan, cell population replication rate, and cell number at confluency of fibroblast cultures derived from the old donor group when compared to parallel cultures from young donors. No significant differences were observed in modal cell volumes and cellular macromolecular contents. The differences observed in cell cultures from old and young donors were quantitatively and qualitatively distinct from those cellular alterations observed in early and late passage WI-38 cells (in vitro "aging"). Therefore, although early and late passage cultures of human diploid cells may provide an important cell system for examining loss of replicative potential, fibroblast cultures derived from old and young human donors may be a more appropriate model system for studying human cellular aging.read more
Citations
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References
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Journal ArticleDOI
The serial cultivation of human diploid cell strains.
Leonard Hayflick,P.S. Moorhead +1 more
TL;DR: A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Journal ArticleDOI
The limited in vitro lifetime of human diploid cell strains
TL;DR: The survival curves obtained with human diploid cell strains are comparable to “multiple-hit” or “ multiple-target” curves obtain with other biological systems where an initial threshold dose is required before an exponential form of the curve is established.
Journal ArticleDOI
The effect of age on creatinine clearance in men: a cross-sectional and longitudinal study.
TL;DR: The decrease in creatinine clearance with age seen in this study represents true renal aging and is not secondary to diseases which become increasingly prevalent in the elderly.
Journal ArticleDOI
Incorporation of 3H-uridine and 3H-uracil into RNA: a simple technique for the detection of mycoplasma contamination of cultured cells.
Edward L. Schneider,Edward L. Schneider,Eric J. Stanbridge,Eric J. Stanbridge,C.J. Epstein,C.J. Epstein +5 more
TL;DR: In this article, a simple technique for the detection of mycoplasma contamination of cultured cells was proposed, where parallel cell cultures were incubated 18 h with 3H-uridine or 3Huracil and the ratio of the specific activities of 3H -uridine labeled RNA to 3HURACil labeled RNA ( UdR U ) was determined.
Journal ArticleDOI
Ageing of Clones of Mammalian Cells
TL;DR: The chief purpose of this article is to review some recent work on the ageing of mammalian cells and to comment on the theoretical interpretation of the experimental findings.