The Ubiquitous Distribution of Late Embryogenesis Abundant Proteins across Cell Compartments in Arabidopsis Offers Tailored Protection against Abiotic Stress
Adrien Candat,Adrien Candat,Gaël Paszkiewicz,Martine Neveu,Romain Gautier,David C. Logan,Marie-Hélène Avelange-Macherel,David Macherel +7 more
TLDR
The broad subcellular distribution of LEA proteins highlights the requirement for each cellular compartment to be provided with protective mechanisms to cope with desiccation or cold stress.Abstract:
Late embryogenesis abundant (LEA) proteins are hydrophilic, mostly intrinsically disordered proteins, which play major roles in desiccation tolerance. In Arabidopsis thaliana, 51 genes encoding LEA proteins clustered into nine families have been inventoried. To increase our understanding of the yet enigmatic functions of these gene families, we report the subcellular location of each protein. Experimental data highlight the limits of in silico predictions for analysis of subcellular localization. Thirty-six LEA proteins localized to the cytosol, with most being able to diffuse into the nucleus. Three proteins were exclusively localized in plastids or mitochondria, while two others were found dually targeted to these organelles. Targeting cleavage sites could be determined for five of these proteins. Three proteins were found to be endoplasmic reticulum (ER) residents, two were vacuolar, and two were secreted. A single protein was identified in pexophagosomes. While most LEA protein families have a unique subcellular localization, members of the LEA_4 family are widely distributed (cytosol, mitochondria, plastid, ER, and pexophagosome) but share the presence of the class A α-helix motif. They are thus expected to establish interactions with various cellular membranes under stress conditions. The broad subcellular distribution of LEA proteins highlights the requirement for each cellular compartment to be provided with protective mechanisms to cope with desiccation or cold stress.read more
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Late seed maturation: drying without dying
TL;DR: Evidence suggesting that molecular players involved in biotic defence also have a regulatory role in seed longevity is presented, and how the concept of plasticity can help understand the acquisition of longevity is explored.
Journal ArticleDOI
Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton
Richard Odongo Magwanga,Richard Odongo Magwanga,Pu Lu,Joy Nyangasi Kirungu,Hejun Lu,Xingxing Wang,Xiaoyan Cai,Zhongli Zhou,Zhang Zhenmei,Haron Salih,Kunbo Wang,Fang Liu +11 more
TL;DR: In this paper, the late embryogenesis abundant (LEA) protein families were characterized in upland cotton and gene expression analysis was carried out to determine their potential role in drought stress tolerance.
Journal ArticleDOI
A Conserved Core of Programmed Cell Death Indicator Genes Discriminates Developmentally and Environmentally Induced Programmed Cell Death in Plants
Yadira Olvera-Carrillo,Michiel Van Bel,Tom Van Hautegem,Matyáš Fendrych,Marlies Huysmans,Maria Simaskova,Matthias Van Durme,Pierre Buscaill,Susana Rivas,Núria S. Coll,Frederik Coppens,Steven Maere,Moritz K. Nowack +12 more
TL;DR: The results indicate that the transcriptional signatures of developmentally controlled cell death are largely distinct from the ones associated with environmentally induced cell death, and an array of specific promoter-reporter lines for developmental PCD in Arabidopsis are established.
Journal ArticleDOI
Genome-wide identification, structural analysis and new insights into late embryogenesis abundant (LEA) gene family formation pattern in Brassica napus.
Yu Liang,Ziyi Xiong,Jianxiao Zheng,Dongyang Xu,Zeyang Zhu,Jun Xiang,Jianping Gan,Nadia Raboanatahiry,Yongtai Yin,Maoteng Li,Maoteng Li +10 more
TL;DR: The results suggest that segmental duplication and whole-genome duplication played a major role in the expansion of the BnLEA gene family in B. napus and provides new insights into the formation of this family.
Journal ArticleDOI
Arabidopsis Seed Mitochondria Are Bioenergetically Active Immediately upon Imbibition and Specialize via Biogenesis in Preparation for Autotrophic Growth
TL;DR: Arabidopsis seed mitochondria are bioenergetically reactivated rapidly after hydration followed by fusion-linked remodeling to form a perinuclear scaffold for membrane biogenesis to support the more energy-demanding processes of cell division and organogenesis of the new seedling.
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