Journal ArticleDOI
Toward improvement of screening through mass spectrometry-based proteomics: ovarian cancer as a case study.
Gordon T Luu,Laura M. Sanchez +1 more
TLDR
This work discusses and provides examples for several workflows employing mass spectrometry-based proteomics towards protein biomarker discovery and characterization in the context of ovarian cancer, and discusses the opportunities to merge these workflows for a multiplexed approach for biomarkers.About:
This article is published in International Journal of Mass Spectrometry.The article was published on 2021-08-04. It has received 4 citations till now. The article focuses on the topics: Biomarker discovery.read more
Citations
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Journal ArticleDOI
Application of Proteomics in Pancreatic Ductal Adenocarcinoma Biomarker Investigations: A Review
Christina Jane Vellan,Jaime Jacqueline Jayapalan,Boon Koon Yoong,Azlina Abdul-Aziz,Sarni Mat-Junit,Perumal Subramanian +5 more
TL;DR: The potential perspective for understanding and identifying the unique alterations in protein expressions that could prove beneficial in discovering new robust biomarkers to detect PDAC at an early stage and ascertain prognosis of patients with the disease are explored.
Posted ContentDOI
An integrated approach to protein discovery and detection from complex biofluids
Gordon T. Luu,Changli Ge,Yisha Tang,Kailiang Li,S. Cologna,Joanna E. Burdette,Judith Su,Laura M. Sanchez +7 more
TL;DR: An untargeted mass spectrometry microprotein profiling method is developed and identified a signature of cystatin A, validated this protein in an animal model, and sought to overcome the limits of detection inherent to mass Spectrometry by demonstrating that cystatar A is present at 100 pM concentrations using a label-free microtoroid resonator.
Journal ArticleDOI
Mass Spectrometry-based Proteomics of Epithelial Ovarian Cancers: a Clinical Perspective.
TL;DR: In this article , the authors reviewed over 2500 interventional clinical trials of ovarian cancers since 1990 and cataloged 22 types of interventions adopted in these trials, including PARP, VEGFR, conventional anti-cancer agents, and the remaining on sex hormones, MEK1/2, PD-L1, ERBB, and FRα.
Journal ArticleDOI
An integrated approach to protein discovery and detection from complex biofluids
TL;DR: In this article , an untargeted mass spectrometry microprotein profiling method was used to identify cystatin A, which was validated in an animal model, and demonstrated that the protein is present at 100 pM concentrations using a label-free microtoroid resonator.
References
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Subjective assessment versus ultrasound models to diagnose ovarian cancer: A systematic review and
J. Kaijser,Roy F.P.M. Kruitwagen,B. Van Calster,Bert Aertgeerts,Jan Y Verbakel,Dirk Timmerman,T Van Gorp +6 more
TL;DR: A two-step strategy of simple rules with subjective assessment for inconclusive tumours yielded best results and matched test performance of expert ultrasound examiners for differentiating benign from malignant adnexal masses prior to surgery.
Journal ArticleDOI
Top-down characterization of endogenous protein complexes with native proteomics.
Owen S. Skinner,Nicole A. Haverland,Luca Fornelli,Rafael D. Melani,Luis H. F. Do Vale,Luis H. F. Do Vale,Henrique S. Seckler,Peter F. Doubleday,Luis F. Schachner,Kristina Srzentić,Neil L. Kelleher,Philip D. Compton +11 more
TL;DR: In this article, a multistage tandem MS approach was used to characterize 125 intact endogenous complexes and 217 distinct proteoforms derived from mouse heart and human cancer cell lines in discovery mode.
Journal ArticleDOI
MALDI imaging and profiling MS of higher mass proteins from tissue.
Alexandra van Remoortere,René J. M. van Zeijl,Nico van den Oever,Julien Franck,Rémi Longuespée,Maxence Wisztorski,Michel Salzet,André M. Deelder,Isabelle Fournier,Liam A. McDonnell +9 more
TL;DR: Increased sensitivity for higher mass proteins is reported by using the CovalX high mass HM1 detector (Zurich, Switzerland), which has been specifically designed for the detection of high mass ions and which is much less prone to detector saturation.
Journal ArticleDOI
Human epididymis protein 4 offers superior specificity in the differentiation of benign and malignant adnexal masses in premenopausal women
TL;DR: In this article, the ability of human epididymis protein 4 (HE4) and CA-125 to distinguish among benign, borderline, and malignant pelvic masses in premenopausal women was evaluated.
Journal ArticleDOI
Analysis of vitamin D metabolic markers by mass spectrometry: Current techniques, limitations of the “gold standard” method, and anticipated future directions
TL;DR: The present review will highlight detrimental influences of the biological matrix, epimer contributions, pitfalls of specific mass spectrometry data acquisition routines (in particular multiple reaction monitoring, MRM), influence of ionization source, derivatization reactions, inter-laboratory comparisons on precision, accuracy, and application range of vitamin D metabolites.