Journal ArticleDOI
Toward improvement of screening through mass spectrometry-based proteomics: ovarian cancer as a case study.
Gordon T Luu,Laura M. Sanchez +1 more
TLDR
This work discusses and provides examples for several workflows employing mass spectrometry-based proteomics towards protein biomarker discovery and characterization in the context of ovarian cancer, and discusses the opportunities to merge these workflows for a multiplexed approach for biomarkers.About:
This article is published in International Journal of Mass Spectrometry.The article was published on 2021-08-04. It has received 4 citations till now. The article focuses on the topics: Biomarker discovery.read more
Citations
More filters
Journal ArticleDOI
Application of Proteomics in Pancreatic Ductal Adenocarcinoma Biomarker Investigations: A Review
Christina Jane Vellan,Jaime Jacqueline Jayapalan,Boon Koon Yoong,Azlina Abdul-Aziz,Sarni Mat-Junit,Perumal Subramanian +5 more
TL;DR: The potential perspective for understanding and identifying the unique alterations in protein expressions that could prove beneficial in discovering new robust biomarkers to detect PDAC at an early stage and ascertain prognosis of patients with the disease are explored.
Posted ContentDOI
An integrated approach to protein discovery and detection from complex biofluids
Gordon T. Luu,Changli Ge,Yisha Tang,Kailiang Li,S. Cologna,Joanna E. Burdette,Judith Su,Laura M. Sanchez +7 more
TL;DR: An untargeted mass spectrometry microprotein profiling method is developed and identified a signature of cystatin A, validated this protein in an animal model, and sought to overcome the limits of detection inherent to mass Spectrometry by demonstrating that cystatar A is present at 100 pM concentrations using a label-free microtoroid resonator.
Journal ArticleDOI
Mass Spectrometry-based Proteomics of Epithelial Ovarian Cancers: a Clinical Perspective.
TL;DR: In this article , the authors reviewed over 2500 interventional clinical trials of ovarian cancers since 1990 and cataloged 22 types of interventions adopted in these trials, including PARP, VEGFR, conventional anti-cancer agents, and the remaining on sex hormones, MEK1/2, PD-L1, ERBB, and FRα.
Journal ArticleDOI
An integrated approach to protein discovery and detection from complex biofluids
TL;DR: In this article , an untargeted mass spectrometry microprotein profiling method was used to identify cystatin A, which was validated in an animal model, and demonstrated that the protein is present at 100 pM concentrations using a label-free microtoroid resonator.
References
More filters
Journal ArticleDOI
A Systematic Investigation of the Best Buffers for Use in Screening by MALDI-Mass Spectrometry.
TL;DR: The most suitable buffers and components, along with their identified optimal concentrations in terms of limiting ion suppression effects, are proposed for use in screening assays measured by MALDI-MS.
Journal ArticleDOI
Understanding Ovarian Cancer: iTRAQ-Based Proteomics for Biomarker Discovery
TL;DR: Five proteins with a strong potential to use in a multimarker test for screening and detection of ovarian cancer are indicated, shedding light on the molecular signaling pathways that are associated with ovarian cancer development.
Journal ArticleDOI
The plasma peptides of ovarian cancer
Jaimie Dufresne,Pete Bowden,Thanusi Thavarajah,Angelique Florentinus-Mefailoski,Zhuo Zhen Chen,Monika Tucholska,Tenzin Norzin,Margaret Truc Ho,Morla Phan,Nargiz Mohamed,Amir Ravandi,Eric Stanton,Arthur S. Slutsky,Claudia C. dos Santos,Alexander D. Romaschin,John C. Marshall,Christina L. Addison,Shawn Malone,Daren K. Heyland,Philip Scheltens,Joep Killestein,Charlotte E. Teunissen,Eleftherios P. Diamandis,K. W. Michael Siu,John Marshall +24 more
TL;DR: Variation in plasma proteins from ovarian cancer may be directly discovered by LC–ESI–MS/MS that will be a powerful tool for clinical research.
Journal ArticleDOI
Diagnostic performances of CA125, HE4, and ROMA index in ovarian cancer.
TL;DR: The sensitivity of HE4 and ROMA index in post menopausal women was higher than premenopausal women for detecting ovarian cancer and in the diagnosis of ovarian cancer, HE4 had higher sensitivity.
Journal ArticleDOI
Stefin B, the major low molecular weight inhibitor in ovarian carcinoma
TL;DR: It is believed that tumor Stefin B does not differ from normal Stefin A and thatogenous cysteine proteinase inhibitors presumably regulate lysosomal cystein endopeptidases, such as cathepsins B and L, in vivo.