Journal ArticleDOI
Toward improvement of screening through mass spectrometry-based proteomics: ovarian cancer as a case study.
Gordon T Luu,Laura M. Sanchez +1 more
TLDR
This work discusses and provides examples for several workflows employing mass spectrometry-based proteomics towards protein biomarker discovery and characterization in the context of ovarian cancer, and discusses the opportunities to merge these workflows for a multiplexed approach for biomarkers.About:
This article is published in International Journal of Mass Spectrometry.The article was published on 2021-08-04. It has received 4 citations till now. The article focuses on the topics: Biomarker discovery.read more
Citations
More filters
Journal ArticleDOI
Application of Proteomics in Pancreatic Ductal Adenocarcinoma Biomarker Investigations: A Review
Christina Jane Vellan,Jaime Jacqueline Jayapalan,Boon Koon Yoong,Azlina Abdul-Aziz,Sarni Mat-Junit,Perumal Subramanian +5 more
TL;DR: The potential perspective for understanding and identifying the unique alterations in protein expressions that could prove beneficial in discovering new robust biomarkers to detect PDAC at an early stage and ascertain prognosis of patients with the disease are explored.
Posted ContentDOI
An integrated approach to protein discovery and detection from complex biofluids
Gordon T. Luu,Changli Ge,Yisha Tang,Kailiang Li,S. Cologna,Joanna E. Burdette,Judith Su,Laura M. Sanchez +7 more
TL;DR: An untargeted mass spectrometry microprotein profiling method is developed and identified a signature of cystatin A, validated this protein in an animal model, and sought to overcome the limits of detection inherent to mass Spectrometry by demonstrating that cystatar A is present at 100 pM concentrations using a label-free microtoroid resonator.
Journal ArticleDOI
Mass Spectrometry-based Proteomics of Epithelial Ovarian Cancers: a Clinical Perspective.
TL;DR: In this article , the authors reviewed over 2500 interventional clinical trials of ovarian cancers since 1990 and cataloged 22 types of interventions adopted in these trials, including PARP, VEGFR, conventional anti-cancer agents, and the remaining on sex hormones, MEK1/2, PD-L1, ERBB, and FRα.
Journal ArticleDOI
An integrated approach to protein discovery and detection from complex biofluids
TL;DR: In this article , an untargeted mass spectrometry microprotein profiling method was used to identify cystatin A, which was validated in an animal model, and demonstrated that the protein is present at 100 pM concentrations using a label-free microtoroid resonator.
References
More filters
Journal ArticleDOI
Statistical design of quantitative mass spectrometry-based proteomic experiments.
Ann L. Oberg,Olga Vitek +1 more
TL;DR: The fundamental principles of statistical experimental design are reviewed, and their application to quantitative mass spectrometry-based proteomics is discussed, and how randomization, replication and blocking help avoid systematic biases due to the experimental procedure are discussed.
Journal Article
Proteomic profiling of urinary proteins in renal cancer by surface enhanced laser desorption ionization and neural-network analysis: identification of key issues affecting potential clinical utility.
Mark A. Rogers,Paul A. Clarke,Jason Noble,Nicholas P. Munro,Alan Paul,Peter Selby,Rosamonde E. Banks +6 more
TL;DR: In this paper, the authors investigated the clinical utility of surface enhanced laser desorption ionization profiling of urine samples in conjunction with neural-network analysis to either detect renal cancer or to identify proteins of potential use as markers, using samples from a total of 218 individuals.
Journal ArticleDOI
Experimental factors affecting the quality and reproducibility of MALDI TOF mass spectra obtained from whole bacteria cells.
TL;DR: The protocol employs a Nd:Yag laser and describes both sample handling and instrument conditions which consistently yield reproducible MALDI TOF mass spectra with greater than 25 peaks from both gram-positive and gram-negative bacteria.
Journal ArticleDOI
Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.
Daniel P. Donnelly,Catherine M. Rawlins,Caroline J. DeHart,Luca Fornelli,Luis F. Schachner,Ziqing Lin,Jennifer L. Lippens,Krishna Aluri,Krishna Aluri,Richa Sarin,Richa Sarin,Bifan Chen,Carter Lantz,Wonhyeuk Jung,Kendall Johnson,Antonius Koller,Jeremy J. Wolff,Iain D. G. Campuzano,Jared R. Auclair,Alexander R. Ivanov,Julian P. Whitelegge,Ljiljana Paša-Tolić,Julia Chamot-Rooke,Paul O. Danis,Lloyd M. Smith,Yury O. Tsybin,Joseph A. Loo,Ying Ge,Neil L. Kelleher,Jeffrey N. Agar +29 more
TL;DR: The Consortium for Top-Down Proteomics presents a decision-tree-based guide to sample preparation and analysis protocols for researchers performing top-down mass-spectrometry-based analysis of intact proteins.
Journal ArticleDOI
Effectiveness of a multivariate index assay in the preoperative assessment of ovarian tumors.
Frederick R. Ueland,Christopher P. DeSimone,Christopher P. DeSimone,Leigh G. Seamon,Rachel A. Miller,Rachel A. Miller,S. Goodrich,S. Goodrich,I. Podzielinski,I. Podzielinski,Lori J. Sokoll,Alan Smith,Alan Smith,John R. van Nagell,John R. van Nagell,Zhen Zhang +15 more
TL;DR: The multivariate index assay demonstrated higher sensitivity and lower specificity compared with physician assessment and CA 125 in detecting ovarian malignancies and was consistent in early- and late-stage cancers.