Journal ArticleDOI
Toward improvement of screening through mass spectrometry-based proteomics: ovarian cancer as a case study.
Gordon T Luu,Laura M. Sanchez +1 more
TLDR
This work discusses and provides examples for several workflows employing mass spectrometry-based proteomics towards protein biomarker discovery and characterization in the context of ovarian cancer, and discusses the opportunities to merge these workflows for a multiplexed approach for biomarkers.About:
This article is published in International Journal of Mass Spectrometry.The article was published on 2021-08-04. It has received 4 citations till now. The article focuses on the topics: Biomarker discovery.read more
Citations
More filters
Journal ArticleDOI
Application of Proteomics in Pancreatic Ductal Adenocarcinoma Biomarker Investigations: A Review
Christina Jane Vellan,Jaime Jacqueline Jayapalan,Boon Koon Yoong,Azlina Abdul-Aziz,Sarni Mat-Junit,Perumal Subramanian +5 more
TL;DR: The potential perspective for understanding and identifying the unique alterations in protein expressions that could prove beneficial in discovering new robust biomarkers to detect PDAC at an early stage and ascertain prognosis of patients with the disease are explored.
Posted ContentDOI
An integrated approach to protein discovery and detection from complex biofluids
Gordon T. Luu,Changli Ge,Yisha Tang,Kailiang Li,S. Cologna,Joanna E. Burdette,Judith Su,Laura M. Sanchez +7 more
TL;DR: An untargeted mass spectrometry microprotein profiling method is developed and identified a signature of cystatin A, validated this protein in an animal model, and sought to overcome the limits of detection inherent to mass Spectrometry by demonstrating that cystatar A is present at 100 pM concentrations using a label-free microtoroid resonator.
Journal ArticleDOI
Mass Spectrometry-based Proteomics of Epithelial Ovarian Cancers: a Clinical Perspective.
TL;DR: In this article , the authors reviewed over 2500 interventional clinical trials of ovarian cancers since 1990 and cataloged 22 types of interventions adopted in these trials, including PARP, VEGFR, conventional anti-cancer agents, and the remaining on sex hormones, MEK1/2, PD-L1, ERBB, and FRα.
Journal ArticleDOI
An integrated approach to protein discovery and detection from complex biofluids
TL;DR: In this article , an untargeted mass spectrometry microprotein profiling method was used to identify cystatin A, which was validated in an animal model, and demonstrated that the protein is present at 100 pM concentrations using a label-free microtoroid resonator.
References
More filters
Journal ArticleDOI
Detection of the Intact GroEL Chaperonin Assembly by Mass Spectrometry
Adam A. Rostom,Carol V. Robinson +1 more
Journal ArticleDOI
Serum human epididymis protein 4 vs carbohydrate antigen 125 for ovarian cancer diagnosis: a systematic review
Simona Ferraro,Federica Braga,Monica Lanzoni,Patrizia Boracchi,Elia Biganzoli,Mauro Panteghini +5 more
TL;DR: HE4 measurement seems to be superior to CA-125 in terms of diagnostic performance for identification of OC in women with suspected gynaecological disease, and more studies tailored on these specific subsets are needed.
Journal ArticleDOI
Effective Use of Mass Spectrometry in the Clinical Laboratory
TL;DR: With increasing economic pressures and decreasing laboratory test reimbursement, mass spectrometry testing has been shown to provide cost-effective solutions and the advancement of this technology along with the development of new applications will only accelerate the incorporation of mass Spectrometry into more areas of medicine.
Journal ArticleDOI
Full-featured, real-time database searching platform enables fast and accurate multiplexed quantitative proteomics
Devin K. Schweppe,Jimmy K. Eng,Qing Yu,Derek J. Bailey,Ramin Rad,José Navarrete-Perea,Edward L. Huttlin,Brian K. Erickson,Joao A. Paulo,Steven P. Gygi +9 more
TL;DR: A new, real-time database search platform, Orbiter, is built to combat the SPS-MS3 method's longer duty cycles, and it is found that RTS enabled a two-fold increase in mass spectrometric data acquisition efficiency.
Journal ArticleDOI
Protein assemblies ejected directly from native membranes yield complexes for mass spectrometry
Dror S. Chorev,Lindsay A Baker,Di Wu,Victoria Beilsten-Edmands,Sarah L. Rouse,Tzviya Zeev-Ben-Mordehai,Chimari Jiko,Firdaus Samsudin,Christoph Gerle,Syma Khalid,Alastair G. Stewart,Alastair G. Stewart,Stephen Matthews,Kay Grünewald,Kay Grünewald,Carol V. Robinson +15 more
TL;DR: In this article, the authors identified chaperone-porin association and lipid interactions in the β-barrel assembly machinery from Escherichia coli outer membranes, and from inner membranes they identified a pentameric pore of TonB, as well as the proteinconducting channel SecYEG in association with F 1 F O adenosine triphosphate (ATP) synthase.