Transfer RNAs: diversity in form and function.
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TLDR
This review analyzes tRNA structure, biosynthesis and function, and includes topics that demonstrate their diversity and growing importance.Abstract:
As the adaptor that decodes mRNA sequence into protein, the basic aspects of tRNA structure and function are central to all studies of biology. Yet the complexities of their properties and cellular roles go beyond the view of tRNAs as static participants in protein synthesis. Detailed analyses through more than 60 years of study have revealed tRNAs to be a fascinatingly diverse group of molecules in form and function, impacting cell biology, physiology, disease and synthetic biology. This review analyzes tRNA structure, biosynthesis and function, and includes topics that demonstrate their diversity and growing importance.read more
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Faculty of 1000 evaluation for A transfer-RNA-derived small RNA regulates ribosome biogenesis.
Journal ArticleDOI
Modifications Modulate Anticodon Loop Dynamics and Codon Recognition in E. coli tRNAArg1,2
Kathryn L. Sarachan,William A. Cantara,Jessica L. Spears,Hasan DeMirci,Frank V. Murphy,Srivathsan V. Ranganathan,Alan A. Chen,Paul F. Agris +7 more
TL;DR: In this paper, the anticodon stem and loop of the ASL(Arg1,2) constructs were analyzed by spectroscopy and codon binding assays, and it was shown that the solution structures of these structures were nearly identical.
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Easy Synthesis of Complex Biomolecular Assemblies: Wheat Germ Cell-Free Protein Expression in Structural Biology
TL;DR: The tremendous potential of the rapidly evolving and highly versatile CFPS systems are highlighted, making them more widely used as common tools to recombinantly prepare particularly challenging recombinant eukaryotic proteins.
Journal ArticleDOI
Identification of Inosine and 2'-O-Methylinosine Modifications in Yeast Messenger RNA by Liquid Chromatography-Tandem Mass Spectrometry Analysis.
TL;DR: Two new modifications of inosine and 2'-O-methylinosine were identified to be prevalent in yeast mRNA, which expends the diversity of RNA modifications in yeast and also suggests new regulators for modulating yeast physiological functions.
Journal ArticleDOI
Recent insights into the structure, function, and regulation of the eukaryotic transfer RNA splicing endonuclease complex
TL;DR: A three‐dimensional model of the human TSEN complex is created using new advancements in the prediction of protein structure and mechanisms for the regulation of tRNA splicing are discussed, by enzymes that modify cleaved tRNA exons and introns.
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