Journal ArticleDOI
Validation of bioanalytical LC-MS/MS assays: evaluation of matrix effects.
TLDR
In this review, the influence of matrix effects on bioanalytical LC-MS/MS methods is discussed and illustrated with some examples, and possible solutions to reduce or eliminate matrix effects are highlighted.About:
This article is published in Journal of Chromatography B.The article was published on 2009-08-01. It has received 692 citations till now. The article focuses on the topics: Ion suppression in liquid chromatography–mass spectrometry & Matrix (chemical analysis).read more
Citations
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Journal ArticleDOI
An overview of matrix effects in liquid chromatography-mass spectrometry.
TL;DR: This review gives a detailed description on when matrix effects (ME) might be expected, and how they can be evaluated, and the main strategies to overcome these phenomena are described in detail.
Journal ArticleDOI
Signal suppression/enhancement in high-performance liquid chromatography tandem mass spectrometry
TL;DR: The review discusses the pitfalls of the matrix effect in mass spectrometry detection hyphenated to liquid chromatography separation and proposes hypotheses to explain the observed behaviours and proposes methods and strategies to overcome the matrix effects.
Journal ArticleDOI
Stir-bar sorptive extraction: A view on method optimisation, novel applications, limitations and potential solutions.
Ailette Prieto,O. Basauri,Rosario Rodil,Aresatz Usobiaga,L.A. Fernández,Nestor Etxebarria,Olatz Zuloaga +6 more
TL;DR: A view on method optimisation, limitations, potential solutions such as in-house coatings and derivatisation and novel applications in multi-residue analysis and passive sampling are revised.
LC-MS/MS in the Clinical Laboratory – Where to From Here?
TL;DR: While sample throughput is higher than for conventional HPLC or GC-MS, it lags behind automated immunoassays, and measures to improve specificity and sensitivity include sample clean-up and optimising chromatography to avoid interferences and ion suppression due to sample-matrix components.
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Reduction of Matrix Effects in Liquid Chromatography–Electrospray Ionization–Mass Spectrometry by Dilution of the Sample Extracts: How Much Dilution is Needed?
TL;DR: It could be shown that a dilution of extracts by a factor of 25-40 reduces ion suppression to less than 20% if the initial suppression is ≤80%, and for stronger matrix effects or complete elimination of suppression, higher dilution factors were needed.
References
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Journal ArticleDOI
Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS.
TL;DR: Practical, experimental approaches for studying, identifying, and eliminating the effect of matrix on the results of quantitative analyses by HPLC-MS/MS are described and it is demonstrated that, for the investigational drug under study, the matrix effect was clearly observed when ISP interface was utilized but it was absent when the HN interface was employed.
Journal ArticleDOI
Ion Suppression in Mass Spectrometry
TL;DR: Options for minimizing or correcting ion suppression are presented, which include enhanced specimen cleanup, chromatographic changes, reagent modifications, and effective internal standardization.
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Matrix effects: the Achilles heel of quantitative high-performance liquid chromatography-electrospray-tandem mass spectrometry.
TL;DR: The major aspects of matrix effects are discussed with an approach to address matrix effects during method validation proposed.
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Mechanistic investigation of ionization suppression in electrospray ionization.
TL;DR: Results point to changes in the droplet solution properties caused by the presence of nonvolatile solutes as the main cause of ionization suppression in electrospray ionization of biological extracts.
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The effects of sample preparation methods on the variability of the electrospray ionization response for model drug compounds.
TL;DR: A post-column infusion system was developed in order to analyze suppression of electrospray ionization (ESI) tandem mass spectrometry response in the presence of endogenous plasma interferences and demonstrated that ESI response suppression is compound dependent.