Journal ArticleDOI
An overview of matrix effects in liquid chromatography-mass spectrometry.
TLDR
This review gives a detailed description on when matrix effects (ME) might be expected, and how they can be evaluated, and the main strategies to overcome these phenomena are described in detail.Abstract:
Matrix-dependent signal suppression or enhancement represents a major drawback in quantitative analysis with liquid chromatography coupled to atmospheric pressure ionization mass spectrometry (LC-API-MS). Because matrix effects (ME) might exert a detrimental impact on important method parameters (limit of detection, limit of quantification, linearity, accuracy, and precision), they have to be tested and evaluated during validation procedure. This review gives a detailed description on when these phenomena might be expected, and how they can be evaluated. The major sources of ME are discussed and illustrated with examples from bioanalytical, pharmaceutical, environmental, and food analysis. Because there is no universal solution for ME, the main strategies to overcome these phenomena are described in detail. Special emphasis is devoted to the sample-preparation procedures as well as to the recent improvements on chromatographic and mass spectrometric conditions. An overview of the main calibration techniques to compensate for ME is also presented. All these solutions can be used alone or in combination to retrieve the performance of the LC-MS for a particular matrix-analyte combination.read more
Citations
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Journal ArticleDOI
Quantification of microenvironmental metabolites in murine cancers reveals determinants of tumor nutrient availability.
Mark R. Sullivan,Laura V. Danai,Laura V. Danai,Caroline A. Lewis,Sze Ham Chan,Dan Y. Gui,Tenzin Kunchok,Emily A Dennstedt,Matthew G. Vander Heiden,Matthew G. Vander Heiden,Alexander Muir,Alexander Muir +11 more
TL;DR: Quantitative metabolomics methods provide a comprehensive characterization of the nutrients present in the tumor microenvironment of widely used models of lung and pancreatic cancer and identify factors that influence metabolite levels in tumors.
Journal ArticleDOI
Reduction of Matrix Effects in Liquid Chromatography–Electrospray Ionization–Mass Spectrometry by Dilution of the Sample Extracts: How Much Dilution is Needed?
TL;DR: It could be shown that a dilution of extracts by a factor of 25-40 reduces ion suppression to less than 20% if the initial suppression is ≤80%, and for stronger matrix effects or complete elimination of suppression, higher dilution factors were needed.
Journal ArticleDOI
Tutorial review on validation of liquid chromatography-mass spectrometry methods: part II.
Anneli Kruve,Riin Rebane,Karin Kipper,Maarja-Liisa Oldekop,Hanno Evard,Koit Herodes,Pekka Ravio,Ivo Leito +7 more
TL;DR: This is the part II of a tutorial review intending to give an overview of the state of the art of method validation in liquid chromatography mass spectrometry and discuss specific issues that arise with MS (and MS-MS) detection in LC (as opposed to the "conventional" detectors).
Journal ArticleDOI
Mass spectrometry-based plant metabolomics: Metabolite responses to abiotic stress.
Tiago F. Jorge,João A. Rodrigues,Camila Caldana,Romy Schmidt,Joost T. van Dongen,Jane Thomas-Oates,Carla António +6 more
TL;DR: Recent applications of metabolomics are described and its increasing application to study plant responses to environmental (stress-) factors, including drought, salt, low oxygen caused by waterlogging or flooding of the soil, temperature, light and oxidative stress are described.
References
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Journal ArticleDOI
Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS.
TL;DR: Practical, experimental approaches for studying, identifying, and eliminating the effect of matrix on the results of quantitative analyses by HPLC-MS/MS are described and it is demonstrated that, for the investigational drug under study, the matrix effect was clearly observed when ISP interface was utilized but it was absent when the HN interface was employed.
Journal ArticleDOI
Analytical properties of the nanoelectrospray ion source.
Matthias Wilm,Matthias Mann +1 more
TL;DR: Improved desolvation in nanoES led to instrument-limited resolution of the signals of a glycoprotein and the ability to signal average extensively allowed the C-terminal sequencing of a 40 kDa protein.
Journal ArticleDOI
Molecular Beams of Macroions
TL;DR: In this article, the authors demonstrate that definite mass/charge states can be formed by electrospraying a dilute polymer solution into an evaporation chamber, negative macroions can be produced and a molecular beam formed by sampling the gaseous mixture of macroions, solvent and nitrogen molecules with a nozzle-skimmer system of the Kantrowitz-Gray type.
Journal ArticleDOI
Ion Suppression in Mass Spectrometry
TL;DR: Options for minimizing or correcting ion suppression are presented, which include enhanced specimen cleanup, chromatographic changes, reagent modifications, and effective internal standardization.
Journal ArticleDOI
On the evaporation of small ions from charged droplets
J. V. Iribarne,B. A. Thomson +1 more
TL;DR: In this paper, a theoretical argument is developed to find in which conditions this ion evaporation can occur; to that end solvation free enthalpies of cluster ions are derived from existing experimental data and the absolute reaction rate theory is applied.