Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients
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It seems that the appropriate surveillance and control measures are essential to prevent the emergence and transmission of XDR A. baumannii in ICU patients, with a high prevalence of biofilm related genes of csuE and pgaB.Abstract:
Nosocomial infections and persistence of multidrug resistant biofilm forming Acinetobacter baumannii in hospitals has made it as a serious problem in healthcare settings worldwide. A total of 100 A. baumannii clinical isolates from immunocompromised patients hospitalized in ICU were investigated for biofilm formation, the presence of biofilm related genes (bap, ompA, csuE, fimH, epsA, blaPER-1, bfmS, ptk, pgaB, csgA, kpsMII), integron characterization and molecular typing based on REP-PCR. All isolates were resistant to three or more categories of antibiotics and considered as multidrug resistant (MDR). A total of 32 isolates were resistant to all tested antibiotics and 91% were extensively drug-resistance (XDR). All isolates were able to produce biofilm and 58% of isolates showed strong ability to biofilm formation. All strong biofilm forming A. baumannii isolates were XDR. All A. baumannii isolates carried at least one biofilm related gene. The most prevalent gene was csuE (100%), followed by pgaB (98%), epsA and ptk (95%), bfmS (92%) and ompA (81%). 98% of isolates carried more than 4 biofilm related genes, simultaneously. Class I integron (67%) was more frequent in comparison with class II (10%) (P < 0.05). The REP-PCR patterns were classified as 8 types (A-H) and 21 subtypes. The A1 (23%) and C1 (15%) clusters were the most prevalent among A. baumannii isolates (P < 0.05). According to the REP-PCR patterns, 23% of all isolates had a clonal relatedness. Our study revealed the high frequency of biofilm forming XDR A. baumannii in ICU patients, with a high prevalence of biofilm related genes of csuE and pgaB. It seems that the appropriate surveillance and control measures are essential to prevent the emergence and transmission of XDR A. baumannii in our country.read more
Citations
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Acinetobacter baumannii Infections in Times of COVID-19 Pandemic.
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Alemu Gedefie,Wondmagegn Demsis,Melaku Ashagrie,Yeshimebet Kassa,Melkam Tesfaye,Mihret Tilahun,Habtye Bisetegn,Zenawork Sahle +7 more
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Proteomics approach to understand bacterial antibiotic resistance strategies.
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Quorum Sensing as a Target for Controlling Surface Associated Motility and Biofilm Formation in Acinetobacter baumannii ATCC® 17978TM.
Celia Mayer,Celia Mayer,Andrea Muras,Ana Parga,Manuel Romero,Soraya Rumbo-Feal,Margarita Poza,José Ramos-Vivas,Ana Otero +8 more
TL;DR: Evidence is provided that a functional QS system is required for surface-associated motility and robust biofilm formation in A. baumannii and that QQ strategies in combination with other enzymatic treatments such as DNase could represent an alternative approach for the prevention and treatment of infections caused by this pathogen.
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Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces
TL;DR: The study demonstrated a high capacity of clinical isolates of MDR A. baumannii to form biofilm and to adhere to respiratory epithelial cells, combined with multidrug resistance, which might contribute to the survival of these organisms and their dissemination in the hospital environment.
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Use of sequence‐based typing and multiplex PCR to identify clonal lineages of outbreak strains of Acinetobacter baumannii
TL;DR: A. baumannii gene sequences were used to design multiplex PCRs to assign isolates belonging to particular genotypes to sequence groups as discussed by the authors, with the exception of closely related alleles within the Group 1 clonal complex, alleles at each locus were highly distinct from each other.
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Relationship between Antibiotic Resistance, Biofilm Formation, and Biofilm-Specific Resistance in Acinetobacter baumannii
Lihua Qi,Hao Li,Chuanfu Zhang,Beibei Liang,Jie Li,Ligui Wang,Xinying Du,Xuelin Liu,Shaofu Qiu,Hongbin Song +9 more
TL;DR: Results from this study imply that biofilm acts as a mechanism for bacteria to get a better survival, especially in isolates with resistance level not high enough, as well as improving understanding of these processes and providing novel insights in the therapeutics and prevention against A. baumannii biofilm-related infections.
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