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Alexander F. Yakunin
Researcher at Bangor University
Publications - 165
Citations - 11962
Alexander F. Yakunin is an academic researcher from Bangor University. The author has contributed to research in topics: Nitrogenase & Phosphatase. The author has an hindex of 46, co-authored 156 publications receiving 9568 citations. Previous affiliations of Alexander F. Yakunin include Université de Montréal & Ontario Institute for Cancer Research.
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Journal ArticleDOI
Evolution and classification of the CRISPR-Cas systems
Kira S. Makarova,Daniel H. Haft,Rodolphe Barrangou,Stan J. J. Brouns,Emmanuelle Charpentier,Philippe Horvath,Sylvain Moineau,Francisco J. M. Mojica,Yuri I. Wolf,Alexander F. Yakunin,John van der Oost,Eugene V. Koonin +11 more
TL;DR: In this paper, the evolutionary relationship between CRISPR-Cas and Cas proteins is analyzed and a unified classification of these systems is proposed based on multiple criteria. But, the classification is based on the phylogenies of the most common cas genes, the sequence and organization of the CRISpr repeats and the architecture of the Cas loci.
Journal ArticleDOI
An updated evolutionary classification of CRISPR–Cas systems
Kira S. Makarova,Yuri I. Wolf,Omer S. Alkhnbashi,Fabrizio Costa,Shiraz A. Shah,Sita J. Saunders,Rodolphe Barrangou,Stan J. J. Brouns,Emmanuelle Charpentier,Daniel H. Haft,Philippe Horvath,Sylvain Moineau,Francisco J. M. Mojica,Rebecca M. Terns,Michael P. Terns,Malcolm F. White,Alexander F. Yakunin,Roger A. Garrett,John van der Oost,Rolf Backofen,Eugene V. Koonin +20 more
TL;DR: An approach combining the analysis of signature protein families and features of the architecture of cas loci that unambiguously partitions most CRISPR–cas loci into distinct classes, types and subtypes is presented.
Journal ArticleDOI
Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants.
Kira S. Makarova,Yuri I. Wolf,Jaime Iranzo,Sergey Shmakov,Omer S. Alkhnbashi,Stan J. J. Brouns,Emmanuelle Charpentier,David R. Cheng,Daniel H. Haft,Philippe Horvath,Sylvain Moineau,Francisco J. M. Mojica,David Scott,Shiraz A. Shah,Virginijus Siksnys,Michael P. Terns,Česlovas Venclovas,Malcolm F. White,Alexander F. Yakunin,Alexander F. Yakunin,Winston X. Yan,Feng Zhang,Roger A. Garrett,Rolf Backofen,John van der Oost,Rodolphe Barrangou,Eugene V. Koonin +26 more
TL;DR: An updated evolutionary classification of CRISPR–Cas systems and cas genes is provided, with an emphasis on the major developments that have occurred since the publication of the latest classification, in 2015, which includes 2 classes, 6 types and 33 subtypes.
Journal ArticleDOI
Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family.
Ekaterina Kuznetsova,Michael Proudfoot,Claudio F. Gonzalez,Greg Brown,Marina V. Omelchenko,Ivan Borozan,Liran Carmel,Yuri I. Wolf,Hirotada Mori,Alexei Savchenko,Alexei Savchenko,Cheryl H. Arrowsmith,Cheryl H. Arrowsmith,Eugene V. Koonin,Aled M. Edwards,Aled M. Edwards,Alexander F. Yakunin,Alexander F. Yakunin +17 more
TL;DR: Using a representative set of 80 phosphorylated substrates, the substrate specificities of 23 soluble HADs encoded in the Escherichia coli genome are characterized and the physiological relevance of the phosphatase activity with the preferred substrate was validated in vivo for one of the HADS, YniC.
Journal ArticleDOI
A dual function of the CRISPR-Cas system in bacterial antivirus immunity and DNA repair
Mohan Babu,Natalia Beloglazova,Robert Flick,Chris Graham,Tatiana Skarina,Boguslaw Nocek,Alla Gagarinova,Oxana Pogoutse,Greg Brown,Andrew Binkowski,Sadhna Phanse,Andrzej Joachimiak,Eugene V. Koonin,Alexei Savchenko,Andrew Emili,Jack Greenblatt,Aled M. Edwards,Alexander F. Yakunin +17 more
TL;DR: The results show that YgbT belongs to a novel, structurally distinct family of nucleases acting on branched DNAs and suggest that, in addition to antiviral immunity, at least some components of the CRISPR–Cas system have a function in DNA repair.