A
Allan Bradley
Researcher at Wellcome Trust Sanger Institute
Publications - 385
Citations - 81969
Allan Bradley is an academic researcher from Wellcome Trust Sanger Institute. The author has contributed to research in topics: Gene & Genome. The author has an hindex of 127, co-authored 379 publications receiving 77492 citations. Previous affiliations of Allan Bradley include Howard Hughes Medical Institute & University of Texas MD Anderson Cancer Center.
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Engineering mouse chromosomes with Cre-loxP: range, efficiency, and somatic applications.
TL;DR: It is concluded that any chromosomal rearrangement can be made in ES cells with the Cre-loxP strategy provided that it does not affect cell viability.
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Compound mutants for the paralogous hoxa-4, hoxb-4, and hoxd-4 genes show more complete homeotic transformations and a dose-dependent increase in the number of vertebrae transformed
Gerald S.B. Horan,Ramiro Ramírez-Solis,Ramiro Ramírez-Solis,Mark S. Featherstone,Debra J. Wolgemuth,Allan Bradley,Richard R. Behringer +6 more
TL;DR: A certain degree of functional redundancy among paralogous genes in specifying regional identity is suggested among single mutant phenotypes alone, such that multiple mutations in these genes result in transformations of vertebrae that are not at their anterior limit of expression.
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Genomic DNA microextraction: A method to screen numerous samples
Ramiro Ramirez-Solis,Jaime A. Rivera-Pérez,James D. Wallace,Marie Wims,Hui Zheng,Allan Bradley +5 more
TL;DR: A rapid protocol to extract high-molecular-weight genomic DNA from a large number of samples and has been used to screen embryonic stem cell clones for the presence of targeted mutations at the Hox-2.6 locus and to obtain data from human blood.
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Target frequency and integration pattern for insertion and replacement vectors in embryonic stem cells.
TL;DR: It is found that the insertion vector targeted up to ninefold more frequently than a replacement vector with the same length of homologous sequence, and the majority of clones targeted with replacement vectors did not recombine as predicted.
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Modifying the mouse: design and desire.
TL;DR: Some recent advances in the ability to construct mice with a variety of genetic modifications are addressed, including an increased understanding of the basic cell biology and in vitro growth characteristics of ES cells, which has facilitated germ line transmission of manipulated clones on a routine basis.