Allison Z. Werner

TL;DR: This work demonstrates increased tolerance towards and growth rate at the expense of hydroxycinnamic acids and presents new targets for improving P. putida for microbial lignin valorization.

TL;DR: This work proposes a mechanism for extracellular nutrient acquisition from aromatic compounds by soil bacteria, which holds promise for improving the efficiency of microbial lignin conversion and suggests that OMVs could potentially be useful tools for synthetic biology and biotechnological applications.

TL;DR: Adaptive evolution is employed to improve muconate production in strains incapable of producing 2-ketogluconate by P. putida in the context of gcd deletion and targeted engineering recapitulate improvements achieved by evolution.

TL;DR: This work shows that metal-catalyzed autoxidation depolymerizes comingled polymers into a mixture of oxygenated small molecules that are advantaged substrates for biological conversion, and engineer a robust soil bacterium to funnel these oxygenated compounds into a single exemplary chemical product.

TL;DR: In this paper, the authors performed four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2IIA3IIBIIA1II from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) TPA hydrolysis to TPA