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Allison Z. Werner
Researcher at National Renewable Energy Laboratory
Publications - 16
Citations - 458
Allison Z. Werner is an academic researcher from National Renewable Energy Laboratory. The author has contributed to research in topics: Pseudomonas putida & Chemistry. The author has an hindex of 5, co-authored 10 publications receiving 122 citations. Previous affiliations of Allison Z. Werner include Colorado State University & Oak Ridge National Laboratory.
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Journal ArticleDOI
Adaptive laboratory evolution of Pseudomonas putida KT2440 improves p-coumaric and ferulic acid catabolism and tolerance
Elsayed Tharwat Tolba Mohamed,Allison Z. Werner,Allison Z. Werner,Davinia Salvachúa,Christine A. Singer,Kiki Szostkiewicz,Manuel Rafael Jiménez-Díaz,Manuel Rafael Jiménez-Díaz,Thomas Eng,Thomas Eng,Mohammad S. Radi,Blake A. Simmons,Blake A. Simmons,Aindrila Mukhopadhyay,Aindrila Mukhopadhyay,Markus J. Herrgård,Steven W. Singer,Steven W. Singer,Gregg T. Beckham,Gregg T. Beckham,Adam M. Feist,Adam M. Feist,Adam M. Feist +22 more
TL;DR: This work demonstrates increased tolerance towards and growth rate at the expense of hydroxycinnamic acids and presents new targets for improving P. putida for microbial lignin valorization.
Journal ArticleDOI
Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440
Davinia Salvachúa,Davinia Salvachúa,Allison Z. Werner,Allison Z. Werner,Isabel Pardo,Martyna Michalska,Brenna A. Black,Bryon S. Donohoe,Stefan J. Haugen,Rui Katahira,Sandra Notonier,Sandra Notonier,Kelsey J. Ramirez,Antonella Amore,Samuel O. Purvine,Erika M. Zink,Paul E. Abraham,Richard J. Giannone,Suresh Poudel,Philip D. Laible,Robert L. Hettich,Gregg T. Beckham,Gregg T. Beckham +22 more
TL;DR: This work proposes a mechanism for extracellular nutrient acquisition from aromatic compounds by soil bacteria, which holds promise for improving the efficiency of microbial lignin conversion and suggests that OMVs could potentially be useful tools for synthetic biology and biotechnological applications.
Journal ArticleDOI
Engineering glucose metabolism for enhanced muconic acid production in Pseudomonas putida KT2440
Gayle J. Bentley,Niju Narayanan,Ramesh K. Jha,Davinia Salvachúa,Joshua R. Elmore,George L. Peabody,Brenna A. Black,Kelsey J. Ramirez,Annette De Capite,William E. Michener,Allison Z. Werner,Dawn M. Klingeman,Heidi S. Schindel,Robert G. Nelson,Lindsey Foust,Adam M. Guss,Taraka Dale,Christopher W. Johnson,Gregg T. Beckham +18 more
TL;DR: Adaptive evolution is employed to improve muconate production in strains incapable of producing 2-ketogluconate by P. putida in the context of gcd deletion and targeted engineering recapitulate improvements achieved by evolution.
Journal ArticleDOI
Mixed plastics waste valorization through tandem chemical oxidation and biological funneling
Kevin P. Sullivan,Allison Z. Werner,Kelsey J. Ramirez,Lucas D. Ellis,Jeremy R. Bussard,Brenna A. Black,David G. Brandner,Felicia Bratti,Bonnie L. Buss,Xueming Dong,Stefan J. Haugen,Morgan A Ingraham,Mikhail O. Konev,William E. Michener,Joel Miscall,Isabel Pardo,Sean P. Woodworth,Adam M. Guss,Yuriy Román-Leshkov,Shannon S. Stahl,Gregg T. Beckham +20 more
TL;DR: This work shows that metal-catalyzed autoxidation depolymerizes comingled polymers into a mixture of oxygenated small molecules that are advantaged substrates for biological conversion, and engineer a robust soil bacterium to funnel these oxygenated compounds into a single exemplary chemical product.
Journal ArticleDOI
Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440.
Allison Z. Werner,Rita Clare,Mand Thomas David,Isabel Pardo,Kelsey J. Ramirez,Stefan J. Haugen,Felicia Bratti,Gara N. Dexter,Joshua R. Elmore,Jay D. Huenemann,George L. Peabody,Christopher W. Johnson,Nicholas A. Rorrer,Davinia Salvachúa,Adam M. Guss,Gregg T. Beckham +15 more
TL;DR: In this paper, the authors performed four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2IIA3IIBIIA1II from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) TPA hydrolysis to TPA