Daniel P. Kiehart

TL;DR: A new microscope using ultrathin light sheets derived from two-dimensional optical lattices is developed, demonstrating the performance advantages of lattice light-sheet microscopy compared with previous techniques and highlighted phenomena that, when seen at increased spatiotemporal detail, may hint at previously unknown biological mechanisms.

TL;DR: The Drosophila embryo is established as an excellent system for the investigation of wound healing because it rapidly and reproducibly heals from both mechanical and ultraviolet laser wounds, even those delivered repeatedly.

TL;DR: The forces that connect the genetic program of development to morphogenesis in Drosophila are investigated and it is found that the bulk of progress toward closure is driven by contractility in supracellular "purse strings" and in the amnioserosa, whereas adhesion-mediated zipping coordinates the forces produced by the purse strings and is essential only for the end stages.

TL;DR: Analysis of cell morphology and myosin localization during dorsal closure in wild-type and homozygous mutant embryos demonstrates a key role for myOSin in the maintenance of cell shape and suggests a model for the involvement of myosIn in cell sheet movement during development.

TL;DR: Grazing incidence structured illumination microscopy (GI-SIM) was developed that is capable of imaging dynamic events near the basal cell cortex at 97-nm resolution and 266 frames/s over thousands of time points, and uncovered new ER remodeling mechanisms, such as hitchhiking of the ER on motile organelles.