Eeva-Liisa Eskelinen

TL;DR: The morphological analysis of PMN is extended using immunogold and freeze fracture electron microscopy to investigate the functions of the highly conserved but poorly understood core autophagic apparatus.

TL;DR: These data provide the first ultrastructural evidence that complement-coated antigens are endocytosed by antigen-nonspecific B cells by CR2 and are delivered to the compartments in which peptide loading for antigen presentation occurs and support the notion that CR2 may play a role in antigen presentation by B cells regardless of B-cell receptor specificity.

TL;DR: High-throughput quantitative proteomics combined with bioinformatics and detailed pathway and network analysis showed that integrins and their cytoplasmic cargo proteins are highly abundant in extracellular vesicles released upon β-glucan stimulation, providing a solid basis for further studies on the functional role of vesicular protein secretion upon fungal infection.

TL;DR: In this paper, the authors used cellular and in-vitro membrane fusion analyses to show that autophagosomes are formed from a hitherto unappreciated hybrid membrane compartment.

TL;DR: It is found that autophagy is impaired in Capns1-deficient cells by immunostaining of the endogenous autophagosome marker LC3 and electron microscopy experiments, and the enhancement of lysosomal activity and long-lived proteins degradation, normally occurring upon starvation, are also reduced.