Comprehensive assessment of T-cell receptor β-chain diversity in αβ T cells
Harlan Robins,Paulo Vidal Campregher,Santosh Srivastava,Abigail Wacher,Cameron J. Turtle,Cameron J. Turtle,Orsalem J. Kahsai,Stanley R. Riddell,Stanley R. Riddell,Edus H. Warren,Edus H. Warren,Christopher S. Carlson +11 more
TLDR
A novel experimental and computational approach is developed to measure TCR CDR3 diversity based on single-molecule DNA sequencing, and it is found that total TCRbeta receptor diversity is at least 4-fold higher than previous estimates, and the diversity in the subset of CD45RO(+) antigen-experienced alphabeta T cells is at at least 10-foldHigher than previously estimates.About:
This article is published in Blood.The article was published on 2009-11-05 and is currently open access. It has received 1074 citations till now. The article focuses on the topics: T cell & Major histocompatibility complex.read more
Citations
More filters
Journal ArticleDOI
PD-1 blockade induces responses by inhibiting adaptive immune resistance
Paul C. Tumeh,Christina L. Harview,Jennifer H. Yearley,I. Peter Shintaku,Emma Taylor,Lidia Robert,Bartosz Chmielowski,Marko Spasic,Gina Henry,Voicu Ciobanu,Alisha N. West,Manuel Carmona,Christine Kivork,Elizabeth Seja,Grace Cherry,Antonio Gutierrez,Tristan Grogan,Christine Mateus,Gorana Tomasic,John A. Glaspy,Ryan O. Emerson,Harlan Robins,Robert H. Pierce,David Elashoff,Caroline Robert,Antoni Ribas +25 more
TL;DR: It is shown that pre-existing CD8+ T cells distinctly located at the invasive tumour margin are associated with expression of the PD-1/PD-L1 immune inhibitory axis and may predict response to therapy.
Journal ArticleDOI
Mismatch repair deficiency predicts response of solid tumors to PD-1 blockade
Dung T. Le,Dung T. Le,Jennifer N. Durham,Jennifer N. Durham,Kellie N. Smith,Hao Wang,Bjarne Bartlett,Bjarne Bartlett,Laveet K. Aulakh,Laveet K. Aulakh,Steve Lu,Steve Lu,Holly Kemberling,Cara Wilt,Brandon Luber,Fay Wong,Fay Wong,Nilofer S. Azad,Agnieszka A. Rucki,Daniel A. Laheru,Ross C. Donehower,Atif Zaheer,George A. Fisher,Todd S. Crocenzi,James J. Lee,Tim F. Greten,Austin G. Duffy,Kristen K. Ciombor,Aleksandra Eyring,Bao H. Lam,Andrew K. Joe,S. Peter Kang,Matthias Holdhoff,Ludmila Danilova,Leslie Cope,Christian F. Meyer,Shibin Zhou,Shibin Zhou,Richard M. Goldberg,Deborah K. Armstrong,Katherine M. Bever,Amanda N. Fader,Janis M. Taube,Franck Housseau,David Spetzler,Nianqing Xiao,Drew M. Pardoll,Nickolas Papadopoulos,Nickolas Papadopoulos,Kenneth W. Kinzler,Kenneth W. Kinzler,James R. Eshleman,Bert Vogelstein,Bert Vogelstein,Robert A. Anders,Robert A. Anders,Luis A. Diaz,Luis A. Diaz +57 more
TL;DR: Evaluating the efficacy of PD-1 blockade in patients with advanced mismatch repair–deficient cancers across 12 different tumor types showed that colorectal cancers with mismatch repair deficiency were sensitive to immune checkpoint blockade with antibodies to programmed death receptor–1 (PD-1).
Journal ArticleDOI
Tumor and Microenvironment Evolution during Immunotherapy with Nivolumab.
Nadeem Riaz,Jonathan J. Havel,Vladimir Makarov,Alexis Desrichard,Walter J. Urba,Jennifer S. Sims,F. Stephen Hodi,Salvador Martín-Algarra,Rajarsi Mandal,William H. Sharfman,Shailender Bhatia,Wen-Jen Hwu,Thomas F. Gajewski,Craig L. Slingluff,Diego Chowell,Sviatoslav M. Kendall,Han Chang,Rachna Shah,Fengshen Kuo,Luc G. T. Morris,John-William Sidhom,Jonathan P. Schneck,Christine Horak,Nils Weinhold,Timothy A. Chan +24 more
TL;DR: Comprehensive genomic profiling data in this study provide insight into nivolumab's mechanism of action and reveal expansion of T cell clones in the setting of neoantigen loss.
Journal ArticleDOI
Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses.
Yonit Lavin,Soma Kobayashi,Andrew Leader,El-ad David Amir,Naama Elefant,Camille Bigenwald,Romain Remark,Robert Sweeney,Christian Becker,Jacob H. Levine,Klaus Meinhof,Andrew Chow,Seunghee Kim-Shulze,Andrea S. Wolf,Chiara Medaglia,Hanjie Li,Julie A. Rytlewski,Ryan O. Emerson,Alexander Solovyov,Benjamin Greenbaum,Catherine Sanders,Marissa Vignali,Mary Beth Beasley,Raja M. Flores,Sacha Gnjatic,Dana Pe'er,Adeeb Rahman,Ido Amit,Miriam Merad +28 more
TL;DR: It is shown that stage I lung adenocarcinoma lesions already harbor significantly altered T cell and NK cell compartments, andaired single-cell analyses thus offer valuable knowledge of tumor-driven immune changes, providing a powerful tool for the rational design of immune therapies.
Journal ArticleDOI
Neoadjuvant anti-PD-1 immunotherapy promotes a survival benefit with intratumoral and systemic immune responses in recurrent glioblastoma.
Timothy F. Cloughesy,Aaron Mochizuki,Joey Orpilla,Willy Hugo,Alexander Lee,Tom B. Davidson,Anthony C. Wang,Benjamin M. Ellingson,Julie A. Rytlewski,Catherine Sanders,Eric S. Kawaguchi,Lin Du,Gang Li,William H. Yong,Sarah C. Gaffey,Adam L. Cohen,Ingo K. Mellinghoff,Eudocia Q. Lee,David A. Reardon,Barbara O’Brien,Nicholas Butowski,Phioanh L. Nghiemphu,Jennifer Clarke,Isabel Arrillaga-Romany,Howard Colman,Thomas Kaley,John de Groot,Linda M. Liau,Patrick Y. Wen,Robert M. Prins +29 more
TL;DR: It is suggested that the neoadjuvant administration of PD-1 blockade enhances both the local and systemic antitumor immune response and may represent a more efficacious approach to the treatment of this uniformly lethal brain tumor.
References
More filters
Journal ArticleDOI
Next-generation DNA sequencing.
Jay Shendure,Hanlee P. Ji +1 more
TL;DR: Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes, transcriptomes and interactomes to become inexpensive, routine and widespread, rather than requiring significant production-scale efforts.
Journal ArticleDOI
The Relation Between the Number of Species and the Number of Individuals in a Random Sample of an Animal Population
TL;DR: It is shown that in a large collection of Lepidoptera captured in Malaya the frequency of the number of species represented by different numbers of individuals fitted somewhat closely to a hyperbola type of curve, so long as only the rarer species were considered.
Journal ArticleDOI
T-cell antigen receptor genes and T-cell recognition.
Mark M. Davis,Pamela J. Bjorkman +1 more
TL;DR: This view of T-cell recognition has implications for how the receptors might be selected in the thymus and how they (and immunoglobulins) may have arisen during evolution.
Journal ArticleDOI
How TCRs Bind MHCs, Peptides, and Coreceptors
TL;DR: The structural basis for MHC restriction and signaling remains elusive as no structural features that define a common binding mode or signaling mechanism have yet been gleaned from the current set of TCR/pMHC complexes.
Journal ArticleDOI
A Direct Estimate of the Human αβ T Cell Receptor Diversity
T. Petteri Arstila,Armanda Casrouge,Véronique Baron,Jos Even,Jean Kanellopoulos,Philippe Kourilsky +5 more
TL;DR: TCR gene amplification and sequencing showed that the naive repertoire is highly diverse, whereas the memory compartment, here one-third of the T cell population, contributes less than 1 percent of the total diversity.