Hilda Hernandez

TL;DR: The use of affinity tagging and purification mass spectrometry is reported to determine systematically the physical interactions of all 18 HIV-1 proteins and polyproteins with host proteins in two different human cell lines (HEK293 and Jurkat).

TL;DR: The systematic proteomic approaches revealed that the ER membrane protein complex (EMC) binds to and promotes the biogenesis of a range of multipass transmembrane proteins, with a particular enrichment for transporters.

TL;DR: It is shown that neurofibromin, the NF1 gene product, is a Spred1-interacting protein that is necessary for Sp red1's inhibitory function, and that Spred 1 binding induces the plasma membrane localization of NF1, which subsequently down-regulates Ras-GTP levels.

TL;DR: A strategy for significantly increasing multiplexing in label-free PRM that takes advantage of the superior separation characteristics and retention time stability of meter-scale monolithic silica-C18 column-based chromatography and shows the utility of the approach in quantifying kinase abundances downstream of previously developed active kinase enrichment methodology based on multidrug inhibitor beads.