Katja Lidschreiber

TL;DR: It is shown that RNA velocity—the time derivative of the gene expression state—can be directly estimated by distinguishing between unspliced and spliced mRNAs in common single-cell RNA sequencing protocols, and expected to greatly aid the analysis of developmental lineages and cellular dynamics, particularly in humans.

TL;DR: New transcriptome alkylation-dependent single-cell RNA sequencing (NASC-seq) is developed, to monitor newly synthesised and pre-existing RNA simultaneously in single cells, and enables precise temporal monitoring of RNA synthesis at single- cell resolution during homoeostasis, perturbation responses and cellular differentiation.

TL;DR: Newly synthesized alkylation-dependent single-cell RNA sequencing (NASC-seq), to monitor both newly synthesized and pre-existing RNA in single cells, is developed and will enable the precise monitoring of RNA synthesis at flexible time periods during homeostasis, perturbation responses and cellular differentiation.

TL;DR: In this article, the authors used transient transcriptome sequencing (TT-seq) to map thousands of transcriptionally active putative enhancers in fourteen human cancer cell lines covering seven types of cancer.

TL;DR: In this article, the authors measured transcriptional activity of DNA sequences that are in aggregate ∼100 times longer than the human genome in three different cell types and classified them into three main types: classical enhancers1, closed chromatin enhancers and chromatin-dependent enhancers.