L
Laura E. Clarke
Researcher at Stanford University
Publications - 11
Citations - 10339
Laura E. Clarke is an academic researcher from Stanford University. The author has contributed to research in topics: Microglia & Astrocyte. The author has an hindex of 9, co-authored 11 publications receiving 6954 citations.
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Journal ArticleDOI
Neurotoxic reactive astrocytes are induced by activated microglia
Shane A. Liddelow,Kevin A. Guttenplan,Laura E. Clarke,Frederick C. Bennett,Christopher J. Bohlen,Lucas Schirmer,Mariko L. Bennett,Alexandra E. Münch,Won-Suk Chung,Todd C. Peterson,Daniel K. Wilton,Arnaud Frouin,Brooke A. Napier,Nikhil Panicker,Manoj Kumar,Marion S. Buckwalter,David H. Rowitch,Valina L. Dawson,Ted M. Dawson,Beth Stevens,Ben A. Barres +20 more
TL;DR: It is shown that activated microglia induce A1 astrocytes by secreting Il-1α, TNF and C1q, and that these cytokines together are necessary and sufficient to induce A2 astroCytes, which are abundant in various human neurodegenerative diseases.
Journal ArticleDOI
Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.
Ye Zhang,Steven A. Sloan,Laura E. Clarke,Christine Caneda,Colton A. Plaza,Paul D. Blumenthal,Hannes Vogel,Gary K. Steinberg,Michael S. B. Edwards,Gordon Li,John A. Duncan,Samuel H. Cheshier,Lawrence M. Shuer,Edward F. Chang,Gerald A. Grant,Melanie Hayden Gephart,Ben A. Barres +16 more
TL;DR: The development of an immunopanning method to acutely purify astrocytes from fetal, juvenile, and adult human brains and to maintain these cells in serum-free cultures is reported, finding that human astroCytes have abilities similar to those of murine astroicytes in promoting neuronal survival, inducing functional synapse formation, and engulfing synaptosomes.
Journal ArticleDOI
Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture
Anca M. Pasca,Steven A. Sloan,Laura E. Clarke,Yuan Tian,Christopher D. Makinson,Nina Huber,Chul Hoon Kim,Jin-Young Park,Nancy A. O'Rourke,Khoa D. Nguyen,Stephen J. Smith,John R. Huguenard,Daniel H. Geschwind,Ben A. Barres,Sergiu P. Paşca +14 more
TL;DR: A simple and reproducible 3D culture approach for generating a laminated cerebral cortex–like structure, named human cortical spheroids (hCSs), from pluripotent stem cells, which demonstrate that cortical neurons participate in network activity and produce complex synaptic events.
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Astrocytes mediate synapse elimination through MEGF10 and MERTK pathways
Won-Suk Chung,Laura E. Clarke,Gordon X. Wang,Benjamin K. Stafford,Alexander Sher,Chandrani Chakraborty,Julia Joung,Lynette C. Foo,Andrew Thompson,Chinfei Chen,Stephen J. Smith,Ben A. Barres +11 more
TL;DR: A novel role for astrocytes in mediating synapse elimination in the developing and adult brain is revealed, MEGF10 and MERTK are identified as critical proteins in the synapse remodelling underlying neural circuit refinement, and have important implications for understanding learning and memory as well as neurological disease processes.
Journal ArticleDOI
Emerging roles of astrocytes in neural circuit development
Laura E. Clarke,Ben A. Barres +1 more
TL;DR: A better understanding of how astrocytes regulate neural circuit development and function in the healthy and diseased brain might lead to the development of therapeutic agents to treat these diseases.