Raju Tomer

TL;DR: Protocols spanning multiple dimensions of the CLARITY workflow are described, ranging from simple, reliable and efficient lipid removal without electrophoretic instrumentation to optimized objectives and integration with light-sheet optics (CLARITY-optimized light- sheet microscopy (COLM)) for accelerating data collection from clarified samples by several orders of magnitude while maintaining or increasing quality and resolution.

TL;DR: Two parallel nigrostriatal dopamine neuron subpopulations differing in biophysical properties, input wiring, output wiring to dorsomedial striatum versus dorsolateral striatum (DLS), and natural activity patterns during free behavior are identified.

TL;DR: This work developed one-photon and multiphoton SiMView implementations and recorded cellular dynamics in entire Drosophila melanogaster embryos with 30-s temporal resolution throughout development and performed high-resolution long-term imaging of the developing nervous system and followed neuroblast cell lineages in vivo.

TL;DR: A catalog of neuronal subclasses provides new understanding of hypothalamic organization and function and distinguished 62 neuronal subtypes producing glutamatergic, dopaminergic or GABAergic markers for synaptic neurotransmission and harboring the ability to engage in task-dependent neurotransmitter switching.

TL;DR: Findings reveal that microRNA evolution and the establishment of tissue identities were closely coupled in bilaterian evolution, and outline a minimum set of cell types and tissues that existed in the protostome–deuterostome ancestor.