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Shaohui Ji
Researcher at Kunming Institute of Zoology
Publications - 24
Citations - 1343
Shaohui Ji is an academic researcher from Kunming Institute of Zoology. The author has contributed to research in topics: Cellular differentiation & Progenitor cell. The author has an hindex of 8, co-authored 23 publications receiving 1221 citations. Previous affiliations of Shaohui Ji include Chinese Academy of Sciences & Shaoxing University.
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Journal ArticleDOI
Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos
Yuyu Niu,Bin Shen,Yiqiang Cui,Yongchang Chen,Jianying Wang,Lei Wang,Yu Kang,Xiaoyang Zhao,Wei Si,Wei Li,Andy Peng Xiang,Jiankui Zhou,Xuejiang Guo,Ye Bi,Chenyang Si,Bian Hu,Guoying Dong,Hong Wang,Zuomin Zhou,Tianqing Li,Tao Tan,Xiuqiong Pu,Fang Wang,Shaohui Ji,Qi Zhou,Xingxu Huang,Weizhi Ji,Jiahao Sha +27 more
TL;DR: By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos, this system successfully achieves precise gene targeting in cynomolgus monkeys and enables simultaneous disruption of two target genes in one step, and no off-target mutagenesis was detected by comprehensive analysis.
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TALEN-Mediated Gene Mutagenesis in Rhesus and Cynomolgus Monkeys
Hailiang Liu,Yongchang Chen,Yuyu Niu,Kunshan Zhang,Yu Kang,Weihong Ge,Xiaojing Liu,Enfeng Zhao,Chencheng Wang,Shaoyun Lin,Bo Jing,Chenyang Si,Quan Lin,Quan Lin,Xiaoying Chen,Xiaoying Chen,Haijun Lin,Xiuqiong Pu,Yingying Wang,Binlian Qin,Fang Wang,Hong Wang,Wei Si,Jing Zhou,Tao Tan,Tianqing Li,Shaohui Ji,Zhigang Xue,Yuping Luo,Liming Cheng,Qi Zhou,Siguang Li,Yi E. Sun,Yi E. Sun,Weizhi Ji +34 more
TL;DR: It is concluded that TALEN-mediated mutagenesis can be an effective tool for genetic modeling of human disease in nonhuman primates.
Journal ArticleDOI
Transgenic rhesus monkeys produced by gene transfer into early-cleavage–stage embryos using a simian immunodeficiency virus-based vector
Yuyu Niu,Yang Yu,Agnieszka Bernat,Shihua Yang,Xiechao He,Xiangyu Guo,Dongliang Chen,Yongchang Chen,Shaohui Ji,Wei Si,Yongqin Lv,Tao Tan,Qiang Wei,Hong Wang,Lei Shi,Jean Guan,Xuemei Zhu,Marielle Afanassieff,Pierre Savatier,Kang Zhang,Qi Zhou,Weizhi Ji +21 more
TL;DR: An improved methodology for the production of transgenic rhesus monkeys is developed, making use of a simian immunodeficiency virus (SIV)-based vector that encodes EGFP and a protocol for infection of early-cleavage–stage embryos, and it is shown that infection does not alter embryo development.
Journal ArticleDOI
The Available Time Window for Embryo Transfer in the Rhesus Monkey (Macaca mulatta)
Yongchang Chen,Yuyu Niu,Shihua Yang,Shihua Yang,Xiechao He,Shaohui Ji,Wei Si,Xianghui Tang,Yunhua Xie,Hong Wang,Yongqing Lu,Qi Zhou,Weizhi Ji +12 more
TL;DR: It is indicated that the available time window for ET in rhesus monkeys is from the late follicular to early luteal phases.
Journal ArticleDOI
Impairments in embryonic genome activation in rhesus monkey somatic cell nuclear transfer embryos.
Yuyu Niu,Shihua Yang,Yang Yu,Chenhui Ding,Jifeng Yang,Shufen Wang,Shaohui Ji,Xiechao He,Yunhua Xie,Xianghui Tang,Qi Zhou,Weizhi Ji +11 more
TL;DR: The data show that SCNT embryos are characterized by abnormal cleavage and the timely onset of embryonic genome transcription, deficits that may explain their reduced pre- and postimplantation developmental capacity.