T
Tianqing Li
Researcher at Kunming University of Science and Technology
Publications - 30
Citations - 1889
Tianqing Li is an academic researcher from Kunming University of Science and Technology. The author has contributed to research in topics: Embryonic stem cell & Stem cell. The author has an hindex of 13, co-authored 22 publications receiving 1542 citations. Previous affiliations of Tianqing Li include Chinese Academy of Sciences & Kunming Institute of Zoology.
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Journal ArticleDOI
Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos
Yuyu Niu,Bin Shen,Yiqiang Cui,Yongchang Chen,Jianying Wang,Lei Wang,Yu Kang,Xiaoyang Zhao,Wei Si,Wei Li,Andy Peng Xiang,Jiankui Zhou,Xuejiang Guo,Ye Bi,Chenyang Si,Bian Hu,Guoying Dong,Hong Wang,Zuomin Zhou,Tianqing Li,Tao Tan,Xiuqiong Pu,Fang Wang,Shaohui Ji,Qi Zhou,Xingxu Huang,Weizhi Ji,Jiahao Sha +27 more
TL;DR: By coinjection of Cas9 mRNA and sgRNAs into one-cell-stage embryos, this system successfully achieves precise gene targeting in cynomolgus monkeys and enables simultaneous disruption of two target genes in one step, and no off-target mutagenesis was detected by comprehensive analysis.
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A developmental landscape of 3D-cultured human pre-gastrulation embryos.
Lifeng Xiang,Yu Yin,Yu Yin,Yun Zheng,Yun Zheng,Yanping Ma,Yonggang Li,Zhigang Zhao,Junqiang Guo,Zongyong Ai,Zongyong Ai,Yuyu Niu,Yuyu Niu,Kui Duan,Kui Duan,Jingjing He,Jingjing He,Shuchao Ren,Dan Wu,Yun Bai,Zhouchun Shang,Xi Dai,Weizhi Ji,Weizhi Ji,Tianqing Li,Tianqing Li +25 more
TL;DR: A 3D culture system to model human embryonic development, together with single-cell transcriptome profiling, provides insights into the molecular developmental landscape during human post-implantation embryogenesis.
Journal ArticleDOI
TALEN-Mediated Gene Mutagenesis in Rhesus and Cynomolgus Monkeys
Hailiang Liu,Yongchang Chen,Yuyu Niu,Kunshan Zhang,Yu Kang,Weihong Ge,Xiaojing Liu,Enfeng Zhao,Chencheng Wang,Shaoyun Lin,Bo Jing,Chenyang Si,Quan Lin,Quan Lin,Xiaoying Chen,Xiaoying Chen,Haijun Lin,Xiuqiong Pu,Yingying Wang,Binlian Qin,Fang Wang,Hong Wang,Wei Si,Jing Zhou,Tao Tan,Tianqing Li,Shaohui Ji,Zhigang Xue,Yuping Luo,Liming Cheng,Qi Zhou,Siguang Li,Yi E. Sun,Yi E. Sun,Weizhi Ji +34 more
TL;DR: It is concluded that TALEN-mediated mutagenesis can be an effective tool for genetic modeling of human disease in nonhuman primates.
Journal ArticleDOI
Generation and Characterization of Rabbit Embryonic Stem Cells
Shufen Wang,Shufen Wang,Xianghui Tang,Yuyu Niu,Yuyu Niu,Hongwei Chen,Hongwei Chen,Bin Li,Bin Li,Tianqing Li,Xiuzhen Zhang,Xiuzhen Zhang,Zhixin Hu,Zhixin Hu,Qi Zhou,Weizhi Ji +15 more
TL;DR: It is shown that pluripotent, stable ESC lines could be derived from fertilized and parthenote‐derived rabbit embryos and their proliferation characteristics were more like those seen in mouse ESCs.
Journal ArticleDOI
Generation of Cynomolgus Monkey Chimeric Fetuses using Embryonic Stem Cells.
Yongchang Chen,Yuyu Niu,Yanjiao Li,Zongyong Ai,Yu Kang,Hong Shi,Zheng Xiang,Zhaohui Yang,Tao Tan,Wei Si,Wei Li,Xueshan Xia,Qi Zhou,Weizhi Ji,Tianqing Li +14 more
TL;DR: It is shown that cynomolgus monkey ESCs grown in adjusted culture conditions are able to incorporate into host embryos and develop into chimeras with contribution in all three germ layers and in germ cell progenitors.