S
Stefan Semrau
Researcher at Leiden University
Publications - 46
Citations - 2227
Stefan Semrau is an academic researcher from Leiden University. The author has contributed to research in topics: Embryonic stem cell & Biology. The author has an hindex of 20, co-authored 37 publications receiving 1698 citations. Previous affiliations of Stefan Semrau include Massachusetts Institute of Technology & RWTH Aachen University.
Papers
More filters
Journal ArticleDOI
Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease
Elisa Giacomelli,Viviana Meraviglia,Giulia Campostrini,Amy Cochrane,Xu Cao,Ruben W.J. van Helden,Ana Krotenberg Garcia,Maria Mircea,Sarantos Kostidis,Richard P. Davis,Berend J. van Meer,Carolina R. Jost,Abraham J. Koster,Hailiang Mei,David G. Míguez,Aat A. Mulder,Mario Ledesma-Terrón,Giulio Pompilio,Luca Sala,Daniela C.F. Salvatori,Roderick C. Slieker,Roderick C. Slieker,Elena Sommariva,Antoine A.F. de Vries,Martin Giera,Stefan Semrau,Leon G.J. Tertoolen,Valeria V. Orlova,Milena Bellin,Milena Bellin,Christine L. Mummery,Christine L. Mummery +31 more
TL;DR: It is shown that tri-cellular combinations of hiPSC-derived CMs, cardiac fibroblasts, and cardiac endothelial cells also enhance maturation in easily constructed, scaffold-free, three-dimensional microtissues (MTs).
Posted ContentDOI
Characterization of directed differentiation by high-throughput single-cell RNA-Seq
TL;DR: An efficient digital gene expression profiling protocol that enables surveying of mRNA in thousands of single cells at a time is developed and applied to profile 12,832 cells collected at multiple time points during directed adipogenic differentiation of human adipose-derived stem/stromal cells in vitro.
Journal ArticleDOI
Differential Stoichiometry among Core Ribosomal Proteins
Nikolai Slavov,Nikolai Slavov,Stefan Semrau,Edoardo M. Airoldi,Bogdan Budnik,Alexander van Oudenaarden +5 more
TL;DR: The existence of ribosomes with distinct protein composition and physiological function is supported by using mass spectrometry to directly quantify RPs across monosomes and polysomes of mouse embryonic stem cells (ESC) and budding yeast.
Journal ArticleDOI
Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells.
Stefan Semrau,Stefan Semrau,Johanna Goldmann,Magali Soumillon,Magali Soumillon,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Rudolf Jaenisch,Alexander van Oudenaarden +8 more
TL;DR: The entry of mouse embryonic stem cells into retinoic acid-mediated differentiation using single cell transcriptomics with high temporal resolution provides a comprehensive analysis of the exit from pluripotency and lineage commitment at the single cell level.
Journal ArticleDOI
Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development.
Mazène Hochane,Patrick R. van den Berg,Xueying Fan,Noémie M. L. P. Bérenger-Currias,Esmée Adegeest,Monika Bialecka,Maaike Nieveen,Maarten Menschaart,Susana M. Chuva de Sousa Lopes,Susana M. Chuva de Sousa Lopes,Stefan Semrau +10 more
TL;DR: A single-cell transcriptomics study of the human fetal kidney is presented, identifying 22 cell types and a host of marker genes and exploring the heterogeneity of the nephrogenic niche, localized podocyte precursors, and confirmed disease-associated marker genes.