抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

Summary Background Erlotinib has been shown to improve progression-free survival compared with chemotherapy when given as first-line treatment for Asian patients with non-small-cell lung cancer (NSCLC) with activating EGFR mutations. We aimed to assess the safety and efficacy of erlotinib compared with standard chemotherapy for first-line treatment of European patients with advanced EGFR-mutation positive NSCLC. Methods We undertook the open-label, randomised phase 3 EURTAC trial at 42 hospitals in France, Italy, and Spain. Eligible participants were adults (>18 years) with NSCLC and EGFR mutations (exon 19 deletion or L858R mutation in exon 21) with no history of chemotherapy for metastatic disease (neoadjuvant or adjuvant chemotherapy ending ≥6 months before study entry was allowed). We randomly allocated participants (1:1) according to a computer-generated allocation schedule to receive oral erlotinib 150 mg per day or 3 week cycles of standard intravenous chemotherapy of cisplatin 75 mg/m 2 on day 1 plus docetaxel (75 mg/m 2 on day 1) or gemcitabine (1250 mg/m 2 on days 1 and 8). Carboplatin (AUC 6 with docetaxel 75 mg/m 2 or AUC 5 with gemcitabine 1000 mg/m 2 ) was allowed in patients unable to have cisplatin. Patients were stratified by EGFR mutation type and Eastern Cooperative Oncology Group performance status (0 vs 1 vs 2). The primary endpoint was progression-free survival (PFS) in the intention-to-treat population. We assessed safety in all patients who received study drug (≥1 dose). This study is registered with ClinicalTrials.gov, number NCT00446225. Findings Between Feb 15, 2007, and Jan 4, 2011, 174 patients with EGFR mutations were enrolled. One patient received treatment before randomisation and was thus withdrawn from the study; of the remaining patients, 86 were randomly assigned to receive erlotinib and 87 to receive standard chemotherapy. The preplanned interim analysis showed that the study met its primary endpoint; enrolment was halted, and full evaluation of the results was recommended. At data cutoff (Jan 26, 2011), median PFS was 9·7 months (95% CI 8·4-12·3) in the erlotinib group, compared with 5·2 months (4·5–5·8) in the standard chemotherapy group (hazard ratio 0·37, 95% CI 0·25–0·54; p vs none of 82 patients in the chemotherapy group), neutropenia (none vs 18 [22%]), anaemia (one [1%] vs three [4%]), and increased amino-transferase concentrations (two [2%] vs 0). Five (6%) patients on erlotinib had treatment-related severe adverse events compared with 16 patients (20%) on chemotherapy. One patient in the erlotinib group and two in the standard chemotherapy group died from treatment-related causes. Interpretation Our findings strengthen the rationale for routine baseline tissue-based assessment of EGFR mutations in patients with NSCLC and for treatment of mutation-positive patients with EGFR tyrosine-kinase inhibitors. Funding Spanish Lung Cancer Group, Roche Farma, Hoffmann-La Roche, and Red Tematica de Investigacion Cooperativa en Cancer.

/pdf/erlotinib-versus-standard-chemotherapy-as-first-line-1hii4s3lek.pdf

Erlotinib versus standard chemotherapy as first-line treatment for European patients with advanced EGFR mutation-positive non-small-cell lung cancer (EURTAC): a multicentre, open-label, randomised phase 3 trial.

https://pubs.rsc.org/en/content/articlepdf/2019/NP/C8NP00092A

Marine natural products.

CA : A Cancer Journal for Clinicians

Background Oncogenic fusion genes consisting of EML4 and anaplastic lymphoma kinase (ALK) are present in a subgroup of non–small-cell lung cancers, representing 2 to 7% of such tumors. We explored the therapeutic efficacy of inhibiting ALK in such tumors in an early-phase clinical trial of crizotinib (PF-02341066), an orally available small-molecule inhibitor of the ALK tyrosine kinase. Methods After screening tumor samples from approximately 1500 patients with non–small-cell lung cancer for the presence of ALK rearrangements, we identified 82 patients with advanced ALK-positive disease who were eligible for the clinical trial. Most of the patients had received previous treatment. These patients were enrolled in an expanded cohort study instituted after phase 1 dose escalation had established a recommended crizotinib dose of 250 mg twice daily in 28-day cycles. Patients were assessed for adverse events and response to therapy. Results Patients with ALK rearrangements tended to be younger than those without the rearrangements, and most of the patients had little or no exposure to tobacco and had adenocarcinomas. At a mean treatment duration of 6.4 months, the overall response rate was 57% (47 of 82 patients, with 46 confirmed partial responses and 1 confirmed complete response); 27 patients (33%) had stable disease. A total of 63 of 82 patients (77%) were continuing to receive crizotinib at the time of data cutoff, and the estimated probability of 6-month progression-free survival was 72%, with no median for the study reached. The drug resulted in grade 1 or 2 (mild) gastrointestinal side effects. Conclusions The inhibition of ALK in lung tumors with the ALK rearrangement resulted in tumor shrinkage or stable disease in most patients. (Funded by Pfizer and others; ClinicalTrials.gov number, NCT00585195.)

/pdf/anaplastic-lymphoma-kinase-inhibition-in-non-small-cell-lung-4chlx92dsn.pdf

Anaplastic Lymphoma Kinase Inhibition in Non–Small-Cell Lung Cancer

Glycosyl Fluoride for Glycosidation Promoted by a Catalytic Amount of Gallium Reagent.

Intrahepatic advance pattern of solitary metastatic liver tumor from colorectal cancer was considered on the basis of the classification according to pre- and intra-operative information in a series of 33 resected cases at the hospital. Small nodule; less than 3cm in tumor diameter, large nodule; more than 3cm, and proliferat-ing nodule; macroscopic existence of marginal proliferating form were defined, and the tumors were classi-fied into the following three types. 1) Small nodular type (S): both 3- and 5-year survival rates were 38%. Remnant liver recurrence was so high in the synchronously metastasized patients, whereas good prognosis in the metachronously metastasized patients. 2) Large nodular type (L): 3- and 5-year survival rates were 66 %and 54% respectively, and no prognostic difference about the distinction of metastasized period was seen. Mean diameter of the tumors specimen was 4.2±1.7cm (mean±S.D.). 3) Proliferating large nodular type (PL): the prognosis was as extremely poor as 1- and 2-year survivals were 33% and 0% respectively. The diameter of the tumor was 9.2±3.5cm, thus PL was considered as the clinically advanced type of L. We believe, that the intrahepatic advance pattern of solitary metastatic liver tumor from colorectal cancer can be pre-dicted pre- and intraoperatively by our classification, and the prediction is properly useful to the therapeutic planning.

Intrahepatic advance pattern of the solitary liver metastasis from colorectal cancer

The first asymmetric synthesis of β-substituted β-methoxyacrylate bearing a chiral 1,2-cis-disubstituted cyclopropane ring as a ‘conformationally locked’ substructure, by Claisen condensation and E-selective Wittig reaction was successfully achieved.

Asymmetric Synthesis of Novel β‐Substituted β‐Methoxyacrylates Bearing a Chiral 1,2‐cis‐Disubstituted Cyclopropane Substructure.

Les acylsilanes α-chiraux montrent des preferences diastereofaciales exceptionnelles dans des additions nucleophiles qui sont suivies d'une protodesilylation par l'anion F − pour donner des alcools secondaires

High Diastereofacial Selectivity in Nucleophilic Additions to Chiral Acylsilanes.

A novel glycosyl donor, glycosyl 2-pyridinecarboxylate, was designed based on a variation of the Remote Activation Concept. Glycosylation using glycosyl 2-pyridinecarboxylate could be effected with copper (II) triflate in Et2O or tin (II) triflate in CH3CN to give α-glucosides or β-glucosides, respectively.

Susumu Kobayashi

Papers

Glycosyl Fluoride for Glycosidation Promoted by a Catalytic Amount of Gallium Reagent.

Intrahepatic advance pattern of the solitary liver metastasis from colorectal cancer

Asymmetric Synthesis of Novel β‐Substituted β‐Methoxyacrylates Bearing a Chiral 1,2‐cis‐Disubstituted Cyclopropane Substructure.

High Diastereofacial Selectivity in Nucleophilic Additions to Chiral Acylsilanes.

A New Glycosylation Reaction Based on a “Remote Activation Concept”: Glycosyl 2‐Pyridinecarboxylate as a Novel Glycosyl Donor.