抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

Summary Background Erlotinib has been shown to improve progression-free survival compared with chemotherapy when given as first-line treatment for Asian patients with non-small-cell lung cancer (NSCLC) with activating EGFR mutations. We aimed to assess the safety and efficacy of erlotinib compared with standard chemotherapy for first-line treatment of European patients with advanced EGFR-mutation positive NSCLC. Methods We undertook the open-label, randomised phase 3 EURTAC trial at 42 hospitals in France, Italy, and Spain. Eligible participants were adults (>18 years) with NSCLC and EGFR mutations (exon 19 deletion or L858R mutation in exon 21) with no history of chemotherapy for metastatic disease (neoadjuvant or adjuvant chemotherapy ending ≥6 months before study entry was allowed). We randomly allocated participants (1:1) according to a computer-generated allocation schedule to receive oral erlotinib 150 mg per day or 3 week cycles of standard intravenous chemotherapy of cisplatin 75 mg/m 2 on day 1 plus docetaxel (75 mg/m 2 on day 1) or gemcitabine (1250 mg/m 2 on days 1 and 8). Carboplatin (AUC 6 with docetaxel 75 mg/m 2 or AUC 5 with gemcitabine 1000 mg/m 2 ) was allowed in patients unable to have cisplatin. Patients were stratified by EGFR mutation type and Eastern Cooperative Oncology Group performance status (0 vs 1 vs 2). The primary endpoint was progression-free survival (PFS) in the intention-to-treat population. We assessed safety in all patients who received study drug (≥1 dose). This study is registered with ClinicalTrials.gov, number NCT00446225. Findings Between Feb 15, 2007, and Jan 4, 2011, 174 patients with EGFR mutations were enrolled. One patient received treatment before randomisation and was thus withdrawn from the study; of the remaining patients, 86 were randomly assigned to receive erlotinib and 87 to receive standard chemotherapy. The preplanned interim analysis showed that the study met its primary endpoint; enrolment was halted, and full evaluation of the results was recommended. At data cutoff (Jan 26, 2011), median PFS was 9·7 months (95% CI 8·4-12·3) in the erlotinib group, compared with 5·2 months (4·5–5·8) in the standard chemotherapy group (hazard ratio 0·37, 95% CI 0·25–0·54; p vs none of 82 patients in the chemotherapy group), neutropenia (none vs 18 [22%]), anaemia (one [1%] vs three [4%]), and increased amino-transferase concentrations (two [2%] vs 0). Five (6%) patients on erlotinib had treatment-related severe adverse events compared with 16 patients (20%) on chemotherapy. One patient in the erlotinib group and two in the standard chemotherapy group died from treatment-related causes. Interpretation Our findings strengthen the rationale for routine baseline tissue-based assessment of EGFR mutations in patients with NSCLC and for treatment of mutation-positive patients with EGFR tyrosine-kinase inhibitors. Funding Spanish Lung Cancer Group, Roche Farma, Hoffmann-La Roche, and Red Tematica de Investigacion Cooperativa en Cancer.

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Erlotinib versus standard chemotherapy as first-line treatment for European patients with advanced EGFR mutation-positive non-small-cell lung cancer (EURTAC): a multicentre, open-label, randomised phase 3 trial.

https://pubs.rsc.org/en/content/articlepdf/2019/NP/C8NP00092A

Marine natural products.

CA : A Cancer Journal for Clinicians

Background Oncogenic fusion genes consisting of EML4 and anaplastic lymphoma kinase (ALK) are present in a subgroup of non–small-cell lung cancers, representing 2 to 7% of such tumors. We explored the therapeutic efficacy of inhibiting ALK in such tumors in an early-phase clinical trial of crizotinib (PF-02341066), an orally available small-molecule inhibitor of the ALK tyrosine kinase. Methods After screening tumor samples from approximately 1500 patients with non–small-cell lung cancer for the presence of ALK rearrangements, we identified 82 patients with advanced ALK-positive disease who were eligible for the clinical trial. Most of the patients had received previous treatment. These patients were enrolled in an expanded cohort study instituted after phase 1 dose escalation had established a recommended crizotinib dose of 250 mg twice daily in 28-day cycles. Patients were assessed for adverse events and response to therapy. Results Patients with ALK rearrangements tended to be younger than those without the rearrangements, and most of the patients had little or no exposure to tobacco and had adenocarcinomas. At a mean treatment duration of 6.4 months, the overall response rate was 57% (47 of 82 patients, with 46 confirmed partial responses and 1 confirmed complete response); 27 patients (33%) had stable disease. A total of 63 of 82 patients (77%) were continuing to receive crizotinib at the time of data cutoff, and the estimated probability of 6-month progression-free survival was 72%, with no median for the study reached. The drug resulted in grade 1 or 2 (mild) gastrointestinal side effects. Conclusions The inhibition of ALK in lung tumors with the ALK rearrangement resulted in tumor shrinkage or stable disease in most patients. (Funded by Pfizer and others; ClinicalTrials.gov number, NCT00585195.)

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Anaplastic Lymphoma Kinase Inhibition in Non–Small-Cell Lung Cancer

Ethyl (E)-3-methyl-2-heptenoate, 6, or ethyl (Z)-3-methyl-2-heptenoate, 8, was prepared in high yield with the perfect retention of configuration by the reaction of ethyl (E)-3-phenylthio-2-butenoate, 5, or (Z)-3-phenylthio-2-butenoate, 7, with n-butylmagnesium bromide and cuprous iodide, respectively.

The stereospecific preparation of trisubstituted olefins

A highly effective surgical technique is required for laparoscopic low rectal transection without sufficient distal margins when ultralow anterior resection (ULAR) with a stapler is a technical challenge for laparoscopic surgery. We report a retrograde single stapling technique (RSST) for laparoscopic ULAR. Total mesorectal excision is performed laparoscopically. After the anal side of the tumor has been closed transanally, the distal line in the right lateral wall of the rectum is partially transected with laparoscopic coagulation shears (LCS). The distal line in the rectum is transected circularly with LCS after detecting the cutting part in the right lateral wall of the rectum laparoscopically. The end of the distal rectum is closed by a purse-string suture using 2-0 prolene transanally. Intestinal resection is performed extracorporeally through a suprapubic incision. After an anvil is placed in the proximal end of the colon over a purse-string suture, it is introduced to the anal canal transabdominally. The purse-string suture is tied to the anvil shaft before connecting it to the center shaft of the circular stapler; the instrument is then fired to create end-to-end anastomosis. For the prevention of anastomotic leakage, a 24-french balloon catheter which decompresses the anastomosis is inserted through the anus.

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Retrograde single stapling technique for laparoscopic ultralow anterior resection

Herein, we describe the first total synthesis of fomitellic acid B (2), a potent inhibitor of DNA polymerases α and β. There are two key features in this synthesis: (i) the AB ring system, with all requisite chiral centers, is stereoselectively constructed by means of titanium(III)-mediated radical cascade cyclization of epoxypolyene; and (ii) an enone moiety in the B-ring is generated by isomerization of an olefin followed by allylic oxidation.

Total Synthesis of Fomitellic Acid B.

Change of the magnetic property of Fe-B-Nd-Nb alloys was investigated with replacing Nb by a glass forming element Zr under constant quenching rate as well as heat treatment conditions. As a result, the coercivity significantly increases up to 1207 kA/m when the half of Nd is replaced by Zr, which is presumably due to grain refinement of the Nd2Fe14B phase. The self-organized nanograin magnets are attractive for future applications since their coercivity can be further improved by reducing the grain size via optimizing the Zr concentration, the quenching rate and the subsequent heat treatment condition.

Synthesis and magnetic properties of nanocomposite magnets self-organized from FeB-Nd-Nb metallic glasses

Synthetic Studies on Antitumor Antibiotic, Bleomycin. Part 32. Man- Designed Bleomycins: Significance of the Binding Sites as Enzyme Models and of the Stereochemistry of the Linker Moiety.

Susumu Kobayashi

Papers

The stereospecific preparation of trisubstituted olefins

Retrograde single stapling technique for laparoscopic ultralow anterior resection

Total Synthesis of Fomitellic Acid B.

Synthesis and magnetic properties of nanocomposite magnets self-organized from FeB-Nd-Nb metallic glasses

Synthetic Studies on Antitumor Antibiotic, Bleomycin. Part 32. Man- Designed Bleomycins: Significance of the Binding Sites as Enzyme Models and of the Stereochemistry of the Linker Moiety.