Thomas Wolf

TL;DR: The thoroughly updated antiSMASH version 4 is presented, which adds several novel features, including prediction of gene cluster boundaries using the ClusterFinder method or the newly integrated CASSIS algorithm, improved substrate specificity prediction for non-ribosomal peptide synthetase adenylation domains based on the new SANDPUMA algorithm, and several usability features have been updated and improved.

TL;DR: CASSIS exploits the idea of co-regulation of the cluster genes, which assumes the existence of common regulatory patterns in the cluster promoters, and searches for ‘islands’ of enriched cluster-specific motifs in the vicinity of anchor genes.

TL;DR: An experimental microevolution approach is used to show that one of the central pathogenicity mechanisms of C. albicans, the yeast-to-hyphae transition, can be subject to experimental evolution, and demonstrates that even central transcriptional networks can be remodeled very quickly under appropriate selection pressure.

TL;DR: This review gives an overview over the latest studies in interspecies interactions made possible by dual RNA-seq, ranging from pathogenic to symbiotic relationships, as well as highlighting potential problems and pitfalls starting from the selection of meaningful time points and number of reads to matters of rRNA depletion.

TL;DR: The growth-retarding activity of the species-specific effectors suggests that host adaptation of R. commune aims at extending the biotrophic stage at the expense of the necrotrophic stage of pathogenesis, and Rhynchosporium may become an object for studying the mutualism-parasitism transition.