Tracy L. Diamond

TL;DR: A multiscale approach to assemble a host-pathogen biochemical network containing 213 confirmed host cellular factors and 11 HIV-1-encoded proteins has uncovered multiprotein virus-host interactions that likely act in concert to facilitate the early steps of HIV- 1 infection.

TL;DR: An “encyclopedia” of all overlaps between studies yielded a more extensively corroborated set of host factors assisting HIV replication, and refined networks that specify cellular subsystems recruited by HIV to assist in replication are reported.

TL;DR: Investigation of the use of artificial tethering interactions to constrain retroviral integration site selection in an in vitro model supports the idea that a direct interaction between LEDGF/p75 and IN can mediate targeting via a tethering mechanism, and provides proof of concept for the ideas that protein-protein interactions might be engineered to Constrain integration site Selection during human gene therapy.

TL;DR: Using the HIV-1 protease binding mode of MK-8718 and PL-100 as inspiration, a novel aspartate binding bicyclic piperazine sulfonamide core was designed and synthesized and showed an 60-fold increase in enzyme binding affinity and a 10- fold increase in antiviral activity relative to MK- 8718.

TL;DR: A sensitive assay for DNA transesterification in which catalysis by human immunodeficiency virus-type 1 (HIV-1) integrase (IN) connects two DNA strands (disintegration reaction), allowing detection using quantitative PCR (qPCR).