William Brabant

TL;DR: The first steps towards a complete reconstruction of the Mycobacterium tuberculosis regulatory network based on ChIP-Seq are taken and system-wide profiling of messenger RNAs, proteins, metabolites and lipids during hypoxia and re-aeration is combined.

TL;DR: In this article, the DNA binding and transcriptional profile of 80% of all predicted M. tuberculosis transcription factors were reported, and wide-spread dormant DNA binding was found across all predicted transcription factors.

TL;DR: A systems-level framework describing the transcriptome of a devastating bacterial pathogen is described, the transcriptional influence of nearly all individual transcription factors in M. tuberculosis is characterized, and the phenotype of a regulator that reduces susceptibility to a first line anti-tubercular drug is validated.

TL;DR: DNA probes are used to measure steady-state cellular pre-16S rRNA pools during growth state transitions in Escherichia coli to support the feasibility of using pre-rRNA-targeted probes to monitor bacterial growth in natural systems, with the caveat that patterns of pre- rRNA depletion vary with the conditions limiting growth.

TL;DR: The generally stable transcriptome described here, and the additional stabilization in response to physiologically relevant stresses, has far-ranging implications for how this pathogen is able to adapt in its human host.