Institution
Anthony Nolan
Nonprofit•London, England, United Kingdom•
About: Anthony Nolan is a nonprofit organization based out in London, England, United Kingdom. It is known for research contribution in the topics: Transplantation & Human leukocyte antigen. The organization has 230 authors who have published 272 publications receiving 13641 citations.
Papers published on a yearly basis
Papers
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TL;DR: HLA class I and II antigens were detected using HLA typing on all cell lines warranting their use as suitable targets for HLA‐restricted cytotoxic T cells.
Abstract: Cell lines RPMI 8226, JJN3, U266 B1, NCI-H929 (all EBV-) and ARH77 and HS-Sultan (both EBV+) have been extensively characterized in this study. EBV- lines expressed the phenotype (CD138-, CD19+, CD20+) whereas EBV+ were (CD138+, CD19-, CD20-). CD56 expression was restricted to EBV- cell lines, with the exception of U266 B1, whereas PCA-1 was strongly expressed on five of the six cell lines. Only EBV+ cell lines bound peanut-agglutinin (PNA). However, all cell lines bound the lectin Jacalin that binds the same receptor as PNA, irrespective of the receptors sialylation status. By RT-PCR and direct sequencing of their IgH V/D/J domains, ARH77 was demonstrated to use the germline sequence VH4-34/dm1/JH6b, whereas no arrangement was demonstrated for RPMI 8226, suggesting IgH gene deletion or mutation. HLA class I and II antigens were detected using HLA typing on all cell lines warranting their use as suitable targets for HLA-restricted cytotoxic T cells. By sensitive RT-PCR, mRNA for IL-6, IL-6R and TNFbeta was found expressed in all cell lines. IL-1 mRNA expression was predominantly associated with the EBV+ phenotype. Although mRNA for IL-3 and GM-CSF was never detected, transcripts for c-kit ligand and, more commonly, its receptor were. Likewise GM-CSF, M-CSF and erythropoietin mRNA transcripts were detected in the majority of cell lines.
44 citations
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TL;DR: The data in this study suggest that for the DPA1-DPB1 heterodimer, the unit of selection is the combined amino acid epitope contributed by both the D PA1 and DPB1 genes, rather than the allele, and that patterns of LD are driven primarily by dimer stability and conformation of the P1 pocket.
Abstract: Here, we present results for DPA1 and DPB1 four-digit allele-level typing in a large (n = 5,944) sample of unrelated European American stem cell donors previously characterized for other class I and class II loci. Examination of genetic data for both chains of the DP heterodimer in the largest cohort to date, at the amino acid epitope, allele, genotype, and haplotype level, allows new insights into the functional units of selection and association for the DP heterodimer. The data in this study suggest that for the DPA1-DPB1 heterodimer, the unit of selection is the combined amino acid epitope contributed by both the DPA1 and DPB1 genes, rather than the allele, and that patterns of LD are driven primarily by dimer stability and conformation of the P1 pocket. This may help explain the differential pattern of allele frequency distribution observed for this locus relative to the other class II loci. These findings further support the notion that allele-level associations in disease and transplantation may not be the most important unit of analysis, and that they should be considered instead in the molecular context.
43 citations
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TL;DR: The results show that the lower responsiveness of CB NK cells to IL-2 is associated with lower levels of expression ofIL-2 receptors and decreased phosphorylation of STAT5 as compared with PB NK cells, and these cytokines should be considered in the future to activate CBNK cells for therapeutic purposes.
42 citations
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TL;DR: A background to unrelated adult donor and recipient safety is provided, a common framework for assessing the health of unrelated adult donors at each stage of the donation pathway is recommended and a novel mechanism for sharing international consensus criteria for individual medical and lifestyle conditions is presented.
Abstract: The World Marrow Donor Association (WMDA) fosters collaboration between international registries to facilitate the exchange of hematopoietic stem cell products for unrelated stem cell donor transplantation. As indications for hematopoietic SCT grow, the movement of products across the world will increase. Although competent authorities may regulate products within their country, there is a need to protect the best interests of donors and recipients by identifying universal donor medical suitability criteria. Within this report the WMDA provides a background to unrelated adult donor and recipient safety, recommends a common framework for assessing the health of unrelated adult donors at each stage of the donation pathway and presents a novel mechanism for sharing international consensus criteria for individual medical and lifestyle conditions. Wherever possible, these criteria are evidence-based. By establishing a donor medical suitability working group, the WMDA has developed a process through which donor centers and registries may request a consensus opinion on conditions not already listed, as well as challenge existing criteria. Guidance from the WMDA is intended to complement, not supersede, guidance from national competent authorities and international regulatory bodies.
42 citations
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Medical University of Vienna1, Swiss Red Cross2, University Hospital of Basel3, Medical University of Graz4, Uppsala University5, National Marrow Donor Program6, Primary Children's Hospital7, Anthony Nolan8, Leiden University9, Dresden University of Technology10, Aichi Medical University11, Leiden University Medical Center12, University of Oxford13, University of Minnesota14, Tokai University15
TL;DR: Different topics intending to support decision-making are covered, with the goal of minimizing medical risk to the donor and protection of the recipient from transmissible diseases.
42 citations
Authors
Showing all 236 results
Name | H-index | Papers | Citations |
---|---|---|---|
Ghulam J. Mufti | 88 | 687 | 30934 |
Nigel H. Russell | 76 | 502 | 21810 |
Steven G.E. Marsh | 65 | 487 | 20763 |
Frits van Rhee | 61 | 323 | 14983 |
Charles Craddock | 60 | 302 | 13660 |
James Robinson | 48 | 172 | 11429 |
John Barrett | 44 | 114 | 7428 |
Bronwen E. Shaw | 42 | 286 | 6191 |
Richard Szydlo | 41 | 166 | 4775 |
Nancy F. Hensel | 34 | 87 | 4754 |
Alejandro Madrigal | 29 | 101 | 3529 |
Lawrence S. Lamb | 28 | 88 | 2311 |
Susana Gómez | 27 | 69 | 1891 |
Ann-Margaret Little | 26 | 63 | 2443 |
J. Alejandro Madrigal | 26 | 105 | 2577 |