Institution
Defence Science and Technology Laboratory
Government•Salisbury, United Kingdom•
About: Defence Science and Technology Laboratory is a government organization based out in Salisbury, United Kingdom. It is known for research contribution in the topics: Burkholderia pseudomallei & Francisella tularensis. The organization has 926 authors who have published 1242 publications receiving 30091 citations. The organization is also known as: Dstl & [dstl].
Papers published on a yearly basis
Papers
More filters
••
TL;DR: Pathway and gene ontology analysis revealed differential expression of functionally important genes; including genes involved in the inflammatory response, cell proliferation, leukocyte extravasation and cholesterol biosynthesis to the EBOV variants.
Abstract: The Ebola virus (EBOV) variant Makona (which emerged in 2013) was the causative agent of the largest outbreak of Ebola Virus Disease recorded. Differences in virus-host interactions between viral variants have potential consequences for transmission, disease severity and mortality. A detailed profile of the cellular changes induced by the Makona variant compared with other Ebola virus variants was lacking. In this study, A549 cells, a human cell line with a robust innate response, were infected with the Makona variant or with the Ecran variant originating from the 1976 outbreak in Central Africa. The abundance of viral and cellular mRNA transcripts was profiled using RNASeq and differential gene expression analysis performed. Differences in effects of each virus on the expression of interferon-stimulated genes were also investigated in A549 NPro cells where the type 1 interferon response had been attenuated. Cellular transcriptomic changes were compared with those induced by human respiratory syncytial virus (HRSV), a virus with a similar genome organisation and replication strategy to EBOV. Pathway and gene ontology analysis revealed differential expression of functionally important genes; including genes involved in the inflammatory response, cell proliferation, leukocyte extravasation and cholesterol biosynthesis. Whilst there was overlap with HRSV, there was unique commonality to the EBOV variants.
20 citations
••
TL;DR: Infection of a murine macrophages-like cell line revealed Ecotin was necessary for the early stages of colonisation allowing replication following cell entry, andAttenuation of the Δeco mutant strain in the murine model of melioidosis further supported Ecotin as a virulence factor of B. pseudomallei.
20 citations
••
TL;DR: Nebulised salbutamol treatment following phosgene induced acute lung injury does not improve survival, and worsens various physiological parameters including arterial oxygen partial pressure and shunt fraction.
Abstract: Objectives To examine the effectiveness of nebulised salbutamol in the treatment of phosgene induced acute lung injury. Method Using previously validated methods, 12 anaesthetised large white pigs were exposed to phosgene (Ct 1978 ± 8 mg min m-3), established on mechanical ventilation and randomised to treatment with either nebulised salbutamol (2.5mg per dose) or saline control. Treatments were given 1, 5, 9, 13, 17 and 21 hours following phosgene exposure. The animals were followed to 24 hours following phosgene exposure. Results Salbutamol treatment had no effect on mortality and had a deleterious effect on arterial oxygenation, shunt fraction and heart rate. There was a reduction in the number of neutrophils from 24.0% ± 4.4 to 12.17% ± 2.1 (p Conclusion Nebulised salbutamol treatment following phosgene induced acute lung injury does not improve survival, and worsens various physiological parameters including arterial oxygen partial pressure and shunt fraction. Salbutamol treatment reduces neutrophil influx into the lung. Its sole use following phosgene exposure is not recommended.
20 citations
••
TL;DR: Convugates of the native and de-O-acetylated CPS with the Hc fragment of tetanus toxin (TetHc) were used as vaccines in a mouse model of melioidosis and suggested that CPS extracted from B. thailandensis can be used as antigen and that the acetyl group is essential for protection.
20 citations
••
TL;DR: A novel application for Fc-SAMs, the detection of molecular interactions, based on the modification of the SAM with target-specific receptors, is proposed, and the detection mechanism is confirmed by measurements of formal potential as a function of electrolyte pH.
Abstract: Ferrocene-terminated self-assembled monolayers (Fc-SAMs) are one of the most studied molecular aggregates on metal electrodes. They are easy to fabricate and provide a stable and reproducible system to investigate the effect of the microenvironment on the electron transfer parameters. We propose a novel application for Fc-SAMs, the detection of molecular interactions, based on the modification of the SAM with target-specific receptors. Mixed SAMs were fabricated by coimmobilization on Au electrodes of thiolated alkane chains with three different head groups: hydroxy terminating head group, ferrocene head group, and a functional head group such as biotin. Upon binding, the intrinsic electric charge of the target (e.g., streptavidin) modifies the electrostatic potential at the plane of electron transfer, causing a shift in the formal potential E°′. The SAMs were characterized by AC voltammetry. The detection mechanism is confirmed by measurements of formal potential as a function of electrolyte pH.
20 citations
Authors
Showing all 928 results
Name | H-index | Papers | Citations |
---|---|---|---|
Richard W. Titball | 79 | 410 | 22484 |
Andrew D. Griffiths | 72 | 152 | 37590 |
Alan D.T. Barrett | 71 | 341 | 17136 |
Jim Haywood | 67 | 213 | 20503 |
Philip N. Bartlett | 58 | 293 | 12798 |
Alan C. Newell | 58 | 209 | 17820 |
David A. Rand | 57 | 223 | 12157 |
Michael P. O'Donnell | 49 | 301 | 8762 |
James Hill | 47 | 216 | 6837 |
Franz Worek | 46 | 262 | 8754 |
Petra C. F. Oyston | 45 | 127 | 7155 |
K. Ravi Acharya | 45 | 161 | 7405 |
Horst Thiermann | 43 | 298 | 7091 |
Leigh T. Canham | 42 | 160 | 18268 |
Mark J. Midwinter | 39 | 180 | 5330 |