Institution
École Polytechnique de Montréal
Education•Montreal, Quebec, Canada•
About: École Polytechnique de Montréal is a education organization based out in Montreal, Quebec, Canada. It is known for research contribution in the topics: Finite element method & Computer science. The organization has 8015 authors who have published 18390 publications receiving 494372 citations.
Papers published on a yearly basis
Papers
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TL;DR: Interior point stabilization is an acceleration method for column generation algorithms that addresses degeneracy and convergence difficulties by selecting a dual solution inside the optimal space rather than retrieving an extreme point.
129 citations
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TL;DR: In this article, the influence of operational parameters such as organic load, pH and temperature on power generation and methane production was studied in a continuous flow air-cathode microbial fuel cell (MFC) seeded with mesophilic anaerobic sludge.
129 citations
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TL;DR: In this article, the recovered poly-3-hydroxyalkanoic acid (PHA) granules were 97 to 98% pure with a molecular weight (MW) between 730,000 and 790,000, depending on the surfactant used.
Abstract: When Alcaligenes eutrophus biomass was treated with a surfactant and then washed with hypochlorite, the recovered poly-3-hydroxyalkanoic acid (PHA) granules were 97 to 98% pure with a molecular weight (MW) between 730,000 and 790,000, depending on the surfactant used. When treated with only surfactant, the MW was slightly higher than that obtained with the surfactant-hypochlorite treatment but the purity was 10% lower. PHA of higher purity but lower MW was obtained with just a hypochlorite treatment.
129 citations
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TL;DR: A femtosecond laser based transfection method using off-resonance plasmonic gold nanoparticles is described, which leads to a very high perforation rate, transfections efficiency three times higher than for conventional lipofection, and very low toxicity.
129 citations
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TL;DR: Perfusion mode titers confirm that the HEK293SF cell is an excellent substrate for high yield production of influenza virus and there is great potential in further improving the production yields through better control of the cell culture environment and viral production kinetics.
Abstract: Cell culture-based production of influenza vaccine remains an attractive alternative to egg-based production. Short response time and high production yields are the key success factors for the broader adoption of cell culture technology for industrial manufacturing of pandemic and seasonal influenza vaccines. Recently, HEK293SF cells have been successfully used to produce influenza viruses, achieving hemagglutinin (HA) and infectious viral particle (IVP) titers in the highest ranges reported to date. In the same study, it was suggested that beyond 4 × 106 cells/mL, viral production was limited by a lack of nutrients or an accumulation of toxic products. To further improve viral titers at high cell densities, perfusion culture mode was evaluated. Productivities of both perfusion and batch culture modes were compared at an infection cell density of 6 × 106 cells/mL. The metabolism, including glycolysis, glutaminolysis and amino acids utilization as well as physiological indicators such as viability and apoptosis were extensively documented for the two modes of culture before and after viral infection to identify potential metabolic limitations. A 3 L bioreactor with a perfusion rate of 0.5 vol/day allowed us to reach maximal titers of 3.3 × 1011 IVP/mL and 4.0 logHA units/mL, corresponding to a total production of 1.0 × 1015 IVP and 7.8 logHA units after 3 days post-infection. Overall, perfusion mode titers were higher by almost one order of magnitude over the batch culture mode of production. This improvement was associated with an activation of the cell metabolism as seen by a 1.5-fold and 4-fold higher consumption rates of glucose and glutamine respectively. A shift in the viral production kinetics was also observed leading to an accumulation of more viable cells with a higher specific production and causing an increase in the total volumetric production of infectious influenza particles. These results confirm that the HEK293SF cell is an excellent substrate for high yield production of influenza virus. Furthermore, there is great potential in further improving the production yields through better control of the cell culture environment and viral production kinetics. Once accomplished, this cell line can be promoted as an industrial platform for cost-effective manufacturing of the influenza seasonal vaccine as well as for periods of peak demand during pandemics.
128 citations
Authors
Showing all 8139 results
Name | H-index | Papers | Citations |
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Yoshua Bengio | 202 | 1033 | 420313 |
Claude Leroy | 135 | 1170 | 88604 |
Lucie Gauthier | 132 | 679 | 64794 |
Reyhaneh Rezvani | 120 | 638 | 61776 |
M. Giunta | 115 | 608 | 66189 |
Alain Dufresne | 111 | 358 | 45904 |
David Brown | 105 | 1257 | 46827 |
Pierre Legendre | 98 | 366 | 82995 |
Michel Bouvier | 97 | 396 | 31267 |
Aharon Gedanken | 96 | 861 | 38974 |
Michel Gendreau | 94 | 456 | 36253 |
Frederick Dallaire | 93 | 475 | 31049 |
Pierre Savard | 93 | 427 | 42186 |
Nader Engheta | 89 | 619 | 35204 |
Ke Wu | 87 | 1242 | 33226 |