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Showing papers by "Federal University of Mato Grosso do Sul published in 2000"


Journal ArticleDOI
TL;DR: An enzyme-linked immunosorbent assay was used to detect antibodies against desmoglein 1 in serum samples from 60 patients with endemic pemphigus foliaceus (fogo selvagem) who lived in Limao Verde or elsewhere in Brazil, 372 normal subjects (without pem PHIaceus), and 126 normal subjects from the United States and Japan.
Abstract: Background Pemphigus foliaceus is an autoimmune skin disease mediated by autoantibodies against desmoglein 1. The endemic form is thought to have an environmental cause. The Terena reservation of Limao Verde in Mato Grosso do Sul, Brazil, is a recently identified focus of the disease, with a prevalence of 3.4 percent in the population. We tested the hypothesis that normal subjects living in an endemic area have antibodies against desmoglein 1. Methods We used an enzyme-linked immunosorbent assay to detect antibodies against desmoglein 1 in serum samples from 60 patients with endemic pemphigus foliaceus (fogo selvagem) who lived in Limao Verde or elsewhere in Brazil, 372 normal subjects (without pemphigus foliaceus) from Limao Verde and surrounding locations, and 126 normal subjects from the United States and Japan. Results Antibodies against desmoglein 1 were detected in 59 of the 60 patients with fogo selvagem (98 percent) but in only 3 of the 126 normal subjects from the United States and Japan (2 perce...

150 citations


Journal ArticleDOI
TL;DR: The reproductive period of each species, breeding sites, clutch and egg sizes, reproductive efforts, effects of female body size parameters on reproductive potential, and the occurrence of parental care and sexual size dimorphism were investigated.
Abstract: A reproductive strategy may be viewed as the combination of physiological, morphological, and behavioral traits that act together to produce the optimal number of offspring under given environmental conditions (Duellman and Trueb, 1986). Thus, different strategies can increase the efficiency or minimize the costs and/or risks associated with reproduction (Harvey and Pagel, 1993). Reproductive effort is an important component of a reproductive strategy because it allows one to measure the costs of reproduction (Stearns, 1992). A great diversity of anurans occur in the American tropics (Duellman, 1988). Nevertheless, there are few detailed studies on the reproduction of these anurans and studies focusing on reproductive effort are even rarer (e.g., Lampo and Medialdea, 1996). For the Pantanal, a great flat plain with an area of approximately 140,000 km2 in southwestern Brazil, there are only preliminary studies on the composition of the anuran fauna and few comments on their reproductive biology (Lutz, 1946, 1972). Based on comparative morphology and behavior, Heyer (1969) divided the genus Leptodactylus into five species groups, where L. chaquensis Cei belongs to the 'ocellatus' group and L. podicipinus (Cope) to the 'melanonotus' group. According to the classification of Duellman and Trueb (1986), L. podicipinus and L. chaquensis have the same reproductive mode: eggs inside a foam nest on the surface of ponds with aquatic feeding larvae. Leptodactylus chaquensis occurs in Argentina, Paraguay, Uruguay, and Brazil (Frost, 1985) and L. podicipinus is found in open formations of Paraguay, Argentina, Bolivia, and central Brazil (Heyer, 1994). In spite of the wide geographical distribution of these species, information about their reproductive biology is scarce (Vizotto, 1967; Gallardo, 1987; Martins, 1996; Rossa-Feres and Jim, 1994, 1996). Our aim was to study the reproductive strategies of L. chaquensis and L. podicipinus, which are very common in the study area. We investigated the reproductive period of each species, breeding sites, clutch and egg sizes, reproductive efforts, effects of female body size parameters on reproductive potential, and the occurrence of parental care and sexual size dimorphism. Data were collected monthly from December 1996 to January 1998. Adults of L. chaquensis and L. podicipinus were hand-captured at night near ponds and flooded areas at Base de Estudos do Pantanal-Universidade Federal de Mato Grosso do Sul (57?00'W; 19?34'S), in the municipal district of Corumba, State of Mato Grosso do Sul, Brazil. The climate is hot and humid, with a dry period from May to September and a rainy season from October to April. Seasonal floodings are common in the area from January to April. Reproductive period was determined based on calling activity of males, presence of amplectant pairs, and/or tadpoles. Breeding sites were characterized according to water depth, presence or absence of vegetation, and type of water body, which were classified as puddles, flooded areas, or permanent ponds. Occurrence of parental care was based on direct observations of the behavior of adults relative to nests and

85 citations


Journal ArticleDOI
TL;DR: First evidence is provided that the great majority of FS patients have circulating T lymphocytes that specifically proliferate in response to the extracellular domain of Dsg1, and long-term T cells developed from these patients also responded to Dsg 1, and this antigen-specific response was shown to be restricted to HLA-DR molecules.
Abstract: Fogo selvagem (FS), the endemic form of pemphigus foliaceus, is a cutaneous autoimmune disease characterized by subcorneal blistering of the epidermis and the production of autoantibodies against the desmosomal antigen desmoglein-1 (Dsg1). Previously, we showed that mice injected with autoantibodies from FS patients develop a skin disease that reproduces the clinical, histological, and immunological features of FS, indicating that autoantibodies play an essential role in the development of this disease. The purpose of this study was to characterize the autoimmune T-cell response associated with FS. We provide here the first evidence, to our knowledge, that the great majority of FS patients have circulating T lymphocytes that specifically proliferate in response to the extracellular domain of Dsg1. Long-term T cells developed from these patients also responded to Dsg1, and this antigen-specific response was shown to be restricted to HLA-DR molecules. These Dsg1-reactive FS T cells exhibited a CD4-positive memory T-cell phenotype and produced a T helper 2-like cytokine profile. These findings represent the initial steps in defining the role of T cells in FS autoimmunity.

83 citations


Journal ArticleDOI
TL;DR: A trypsin inhibitor from Dimorphandra mollis seeds was isolated to apparent homogeneity by a combination of ammonium sulfate precipitation, gel filtration, ion-exchange and affinity chromatographic techniques.

73 citations


Journal ArticleDOI
TL;DR: Results suggest that in some cases sodium chloride intoxication/water deprivation could play a role in the etiology of the disease, which affected cattle from 4 months to 7 years of age.
Abstract: Thirty outbreaks of polioencephalomalacia (PEM) were diagnosed from August 1993 to October 1997. Twenty nine occurred in the state of Mato Grosso do Sul and one in Sao Paulo. The disease affected cattle from 4 months to 7 years of age. Morbidity rates ranged from 0.02% to 14.28% and case fatalities from 42.5 % to 100%. The disease was not seasonal. All outbreaks occurred in cattle grazing Brachiaria spp, except one affecting feedlot cattle. Only neurologic signs were observed, and the course of the disease varied from 12 hours to 4 days in untreated cattle. Most animals treated with thiamine and dexamethazone recovered. Moderate cerebral edema was observed at necropsy. Cell changes were characterized by laminar necrosis and gitter cells. In two cases moderate cortical and submeningeal hemorrhages were observed. Nine cases had perivascular and submeningeal infiltrations by eosinophils. Sodium ion concentration of the cerebrospinal fluid was elevated in one case. The cause of PEM in the region was not determined, but the results suggest that in some cases sodium chloride intoxication/water deprivation could play a role in the etiology of the disease. PEM represents 4.78% of cattle disease diagnosed at the Pathology Laboratory of the Federal University of Mato Grosso do Sul.

32 citations


Journal ArticleDOI
TL;DR: Two saprophytic fungi were tested for their ability to induce phytoalexin production by seeds of frog-eye leaf spot and stem canker-resistant and -susceptible soybean cultivars and genistein was found to be an inducibly formed isoflavonoid instead of a constitutive metabolite in the resistant cultivar.
Abstract: Two saprophytic fungi (Mucor ramosissimus and Rhizopus sp.) were tested for their ability to induce phytoalexin production by seeds of frog-eye leaf spot and stem canker-resistant and -susceptible soybean (Glycine max L.) cultivars. Only M. ramosissimus was shown to elicit a response and qualitative differences in phytoalexin accumulation were found between the susceptible and resistant cultivars. Glyceollins I, II, and III and glycinol were isolated from the susceptible cultivar, whereas Glyceollins I, II, and III, glycinol, glyceocarpin, genistein, isoformononetin, and N-acetyltyramine accumulated in the resistant cultivar in response to the same fungal elicitor. Genistein was found to be an inducibly formed isoflavonoid instead of a constitutive metabolite in the resistant cultivar, whereas N-acetyltyramine is described for the first time as a soybean phytoalexin. All the compounds, except genistein, showed fungitoxic activity against Cladosporium sphaerospermum. Spectral data of the pterocarpan phytoalexins, genistein, and N-acetyltyramine are also given in this work.

31 citations


Journal ArticleDOI
TL;DR: A solid-phase extraction and chromatography-flame ionization detection (GC-FID) method has been developed for the routine analysis of psoralen, bergapten, isopimpinellin and pimpineellin in creams and pomades employed in Brazil for the treatment of vitiligo.

29 citations


Journal ArticleDOI
TL;DR: Glucose, albumin, aspartate aminotransferase, and alkaline phosphatase levels were higher during spring and summer (rainy season) than during fall and winter (dry season).
Abstract: Em um esquema de parcela subdividida que incluia dois tratamentos (A= solo arenoso e B= solo argiloso) e 10 subtratamentos (10 epocas de amostragem) a bioquimica clinica de 20 vacas de corte, sob pastejo continuo de B. decumbens, foi acompanhada durante 13 meses. Os constituintes sanguineos estudados foram: glicose (GLI), hemoglobina (HB), bilirrubina (BIL), creatinina (CREA), albumina (ALB), aspartatoaminotransferase (AST), fosfatase alcalina (FAS) e creatinoquinase (CK). Foram obtidos valores medios de 98,0 e 110,2 mg/dl para GLI, 13,27 e13,38g/l para HB, 0,718 e 0,661mg/dl para BIL, 1,89 e 1,73mg/dl para CREA e 3,56 e 3,39g/dl para ALB nos tratamentos A e B, respectivamente. Vacas aneloradas apresentaram um perfil bioquimico clinico de animais aparentemente sadios, isto e, ausencia de anemia, ictericia e disfuncao renal. Nao foi possivel diagnosticar alteracoes hepaticas mas ocorreram lesoes musculares (CK) em algumas epocas, sem afetar clinicamente os animais. GLI, ALB, AST e FAS apresentaram mudanca sazonal com niveis mais altos na primavera-verao e mais baixos no periodo de seca, no outono-inverno.

26 citations


Journal ArticleDOI
TL;DR: In this article, two different trials were designed: Trail 1 (E1) and Trial 2 (E2) to evaluate the reproductive performance of males and females submitted to two different air speed limits - FV1, from 0.03 to 0.26 m/sec, and FV2,from 0.27 to0.80 m / sec.
Abstract: Previous studies reported that rats (Rattus norvegicus) kept under microenvironmental ventilation systems (MEV) present better productive and health parameters when compared to animals kept under general diluting ventilation (GDV). The objective of the present research trial was to evaluate hematological and biochemical profiles of rats kept under MVS. In order to achieve this objective, two different trials were designed: Trail 1 (E1), in which it was evaluated the reproductive performance of males and females submitted to two different air speed limits - FV1, from 0.03 to 0.26 m / sec and FV2, from 0.27 to 0.80 m / sec. In Trial 2 (E2) it was evaluated different bed change intervals (3, 5, 7 and 9 days), for males kept under constant air speed (0.5 m / sec). Values for hemogram and biochemical patterns of these animals were compared to those of rats kept under GDV. Results show statistical differences in some of the studied parameters not only for the comparison between GVD and E1 and GVD and E2, but also between both groups submitted to MEV (E1 and E2). However, values found for all studied parameters are inside the normal range reported for this species, what indicates that MEV does not induce important changes in the physiological parameters evaluated.

25 citations


Journal ArticleDOI
TL;DR: In this paper, a pesquisa insere-se na abordagem historico-cultural, that toma como referencia os trabalhos de Vygotsky and Bakthin a respeito da constituicao da subjetividade como um processo de apropriacao de relacoes sociais.
Abstract: Este estudo propoe-se a identificar e analisar, na fala de uma pessoa com diagnostico de deficiencia multipla, aspectos do pensamento coletivo. Nossa pesquisa insere-se na abordagem historico-cultural, que toma como referencia os trabalhos de Vygotsky e Bakthin a respeito da constituicao da subjetividade como um processo de apropriacao de relacoes sociais. O trabalho empirico desenvolveu-se em uma instituicao particular de carater assistencial para atendimento de pessoas com deficiencia multipla no estado de Sao Paulo. Enfocamos a dinâmica discursiva de uma pessoa com deficiencia multipla, apresentando-a marcada por condicoes macroestruturais de producao e discutimos algumas implicacoes na relacao ensino-aprendizagem.

24 citations


Journal ArticleDOI
TL;DR: Results indicate that S. auriculata exhibit density-dependent morphological plasticity, and suggests that the increase in production of sporocarps may not be a simple response to the size of ramets.

Journal ArticleDOI
TL;DR: The prevalence of C. parvum among infants of less than 5 years of age, resident in the urban area of Campo Grande, Mato Grosso do Sul State, Brazil, 1996/97 was established through parasitological examinations and epidemiological analysis of the diagnosed cases.
Abstract: The purpose of this study was to establish the prevalence of Cryptosporidium parvum among infants of less than 5 years of age, resident in the urban area of Campo Grande, Mato Grosso do Sul State, Brazil, 1996/97, through parasitological examinations and epidemiological analysis of the diagnosed cases. It was a transverse study with domiciliary inquiry and we evaluated 1051 fecal samples processed by the Blagg method, and a modified Ziehl-Neelsen staining was used for research of oocysts of C. parvum. We can concluded that the prevalence of C. parvum (1.1%) was not statistically significant; 58.3% of the children with positive diagnosis presented diarrhea, suggesting an association between this sign and the presence of the parasites; C. parvum was more frequent among children aged between 25 to 36 months (50%), but this was not statistically significant; sex did not have a differential role in relation to the cryptosporidiosis; out of the 12 children with cryptosporidiosis, 10 had contact with domestic animals (dogs and or cats).

Journal ArticleDOI
TL;DR: The authors discute a atuacao do psicologo na educacao, na perspectiva da promocao de saude, entendida como uma construcao historica do homem que se relaciona com seus modos de vida, indo alem do binomio saude/doenca.
Abstract: Este artigo discute a atuacao do psicologo na educacao, na perspectiva da promocao de saude, entendida como uma construcao historica do homem que se relaciona com seus modos de vida, indo alem do binomio saude/doenca. Nessa perspectiva, as acoes do psicologo na educacao enfatizam o compromisso etico e politico do acesso a educacao enquanto um bem cultural fundamental para a construcao da cidadania das criancas e jovens brasileiros, entendida como um projeto de promocao de saude.

Journal ArticleDOI
TL;DR: In this paper, a triterpenoid structure was elucidated by NMR spectroscopy as 2a,3b,6b-trihydroxyolean-12-en-28-oic acid (6bhydroxymaslinic acid, 1).
Abstract: A novel oleane acid was isolated from the leaves and the fruits of Vochysia ferruginea. The structure of the new triterpenoid was elucidated by NMR spectroscopy as 2a,3b,6b-trihydroxyolean-12-en-28-oic acid (6b-hydroxymaslinic acid, 1). In addition, b-sitosterol-glucoside and three mixtures containing known triterpenoids, uvaol and erythrodiol, ursolic and oleanolic acids, 2a,3b-dihydroxyurs-12-en-28-oic acid and its respective oleanolic isomer (maslinic or crategolic acid), were isolated from the leaves and the fruits of Vochysia ferruginea. In the fruits, bellericagenin A and its (28 ® 1) b-D-glucopyranosyl ester (bellericaside A) were present in high amount.

Journal ArticleDOI
TL;DR: This is the first report of the natural occurrence of C-7 β-substituted limonoids without any oxygenated function at C-6, and the structures of these compounds have been established on the basis of 1D- and 2D-NMR spectral techniques, ESI-mass spectrum and X-ray crystallographic data.

Journal ArticleDOI
TL;DR: In this paper, the authors performed a study of mechanisms related to ac electrical conductivity of virgin and aged low-density polyethylene (LDPE) loaded with different concentrations of carbon black (CB).
Abstract: We performed a study of mechanisms related to ac electrical conductivity of virgin and aged low-density polyethylene (LDPE) loaded with different concentrations of carbon black (CB). Experimental ac conductivity measurements were carried out at different temperatures on samples with different CB concentrations. Scanning calorimetry (DSC) and thermal expansion measurements were used as auxiliary techniques in this study. We also investigated the aging effect of UV radiation on the electrical properties of the samples. Based on the distribution of the CB in the polyethylene matrix, the ac conductivity is explained in terms of the hopping mechanism considering the material as a system where the energy potential barriers are distributed randomly in the bulk of the material.

Journal ArticleDOI
TL;DR: In this paper, the authors tested quatro cultivares of soja consorciados with milho in dois arranjos culturais: linhas alternadas de milho e soja, or linha de soja alternada with duas linhas of soga, em delineamento de blocos ao acaso.
Abstract: Com o objetivo de produzir silagens mais proteicas, foram testados quatro cultivares de soja consorciados com milho em dois arranjos culturais: linhas alternadas de milho e soja ou linha de milho alternada com duas linhas de soja, em delineamento de blocos ao acaso. Mediram-se as producoes de MS e de PB e determinou-se a qualidade das silagens. A producao de MS e de PB nao foi aumentada no arranjo com maior concentracao de soja. As silagens apresentaram reduzido teor de MS, pH normal e elevada porcentagem de PB. Foi recomendado para a regiao o arranjo alternado de linhas de milho e soja, utilizando os cultivares Dourados e Ocepar-9 e tambem que o corte das forrageiras para ensilagem fosse mais tardio, quando o milho estivesse no estadio fisiologico de farinaceo para semiduro.

Journal ArticleDOI
TL;DR: The importance of these non genetic effects on body weight of calf at 205 days of age indicates the need to consider them when estimating genetic parameters and breeding values for selection purpose.
Abstract: Este trabalho teve o objetivo de estudar a influencia de efeitos nao geneticos sobre o peso ao desmame de 105.465 bezerros da raca Nelore, nascidos em oito diferentes regioes brasileiras, no periodo de 1978 a 1994. A analise estatistica foi realizada utilizando-se o metodo dos quadrados minimos, procedimento GLM (SAS, 1996), com um modelo estatistico contendo como fontes de variacao os efeitos fixos de sexo, mes e ano de nascimento do bezerro, regiao, fazenda dentro de regiao, grupo de idade da vaca e, como efeitos aleatorios, touro e o erro. Todas as fontes de variacao do modelo foram significativas (P<0,0001). A importância desses efeitos nao geneticos sobre o peso aos 205 dias de idade de bezerros da raca Nelore evidenciam a necessidade de se fazer ajustes para os mesmos, visto que estes influenciaram sobre a caracteristica estudada.

Journal ArticleDOI
TL;DR: The turnover rates of the 13C in egg and liver tissues of adult laying hens are established through the substitution of commercial diets by diets containing C3 and C4 photosynthetic cycle grains for 50 days.
Abstract: The stable carbon isotopes used in ecology and paleoecology during the last 30 years has now been used in dietary studies of animals However, there are not enough studies on the metabolism patterns and turnover rates of the 13C in avian This experiment established the turnover rates of the 13C in egg and liver tissues of adult laying hens through the substitution of commercial diets by diets containing C3 and C4 photosynthetic cycle grains for 50 days The d‰13C difference in two diets contents was 1613‰ The diets 13C turnover rates in tissues were adapted in an exponential model that describes the isotopic carbon turnover in those tissues Avian turnover rates of 13C fed with diet based on grains C3 were larger in liver than egg with half-life of 29 and 37 days Avian fed with diet based on grains C4 showed turnover rates larger in egg than liver, with half-life of 40 to 53 days respectively The d‰13C values reached in egg and liver differed of the diet in approximately 2‰

Journal ArticleDOI
TL;DR: The case of a patient with colloid cyst of third ventricle located in a retroforaminal position, in the diencefalic roof, dissecting the raphe fornix, expanding supero-posteriorly, inside the cavum of the septum pellucidum, protruding on the floor of lateral ventricles is reported.
Abstract: The endoscopic treatment of colloid cysts of third ventricle is still controversial. Management of the patient can include observation and serial CT or MRI imaging only, ventriculo peritoneal shunt only, estereotactic aspiration, microsurgery and endoscopy. The author reports the case of a patient with colloid cyst of third ventricle located in a retroforaminal position, in the diencefalic roof, dissecting the raphe fornix, expanding supero-posteriorly, inside the cavum of the septum pellucidum, protruding on the floor of lateral ventricles. The cyst was approached with a rigid neuroendoscope, through a pre-coronal burr hole, 2.5 cm lateral to the midline (Kocher´s point). Following a transventricular route, the right leaf of the septum pellucidum was endoscopically opened with bipolar, posterior to the septal vein. A yellowish hard lesion was found and partially resected with biopsy forceps, between the two fornices, which were split apart by the uncommon expansion and location of the lesion, allowing a new endoscopic transventricular transepto-interforniceal approach. We also performed endoscopic septostomy and third ventriculostomy. The microsurgical approaches compared with endoscopic approaches, and the possibility of endoscopic alternative routes suitable for selected cases with superior expansion towards the fornix and septum pellucidum is discussed.

Journal ArticleDOI
TL;DR: Qualitative research under a phenomenological perspective allows a reflection about significant learning in nursing, generating a new view as a consequence to the student feelings, thoughts and actions in the promotion of the personal and professional growth.
Abstract: This present study, by applying qualitative research under a phenomenological perspective, attemps to seek an understanding of the meaning of the learning experiences of nursing students who care for child and family in the community. The data have been obtained through individual open interviews with nine students. Two' themes have emerged from the analysis of the data: FACING CHALLENGES and GENERATING A NEW VIEW. Applying the theoretical approach of Viktor Frankl's existencial analysis allows us to understand that what mobilizes students when facing challenges is the search for knowledge and desire to do something. Moreover, this study allows a reflection about significant learning in nursing, generating a new view as a consequence to the student feelings, thoughts and actions in the promotion of the personal and professional growth.

Journal ArticleDOI
TL;DR: The toxicity of a hydroethanol extract of the subterranean part of Cochlospermum regium was evaluated in mice and rats and revealed that the extract is well tolerated by these animals.
Abstract: The toxicity of a hydroethanol extract of the subterranean part of Cochlospermum regium was evaluated in mice and rats. The extract had moderate acute toxicity when administered intraperitoneally and low toxicity upon oral administration. A subacute toxicity test revealed that the extract is well tolerated by these animals.

Journal ArticleDOI
TL;DR: Ozonolysis of maleopimaric anhydride methyl ester in the presence of tetracyanoethylene led to an epoxide and an ozonide as discussed by the authors.
Abstract: Ozonolysis of maleopimaric anhydride methyl ester in the presence of tetracyanoethylene led to an epoxide and an ozonide. Ozonolysis of the trimethyl fumaropimarate, followed by treatment with Me2S, led to an epoxide, a diene, a keto-acid and an allylic oxidation product. Some of the compounds obtained were active against Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus.

Journal ArticleDOI
TL;DR: Three Brazilian species of the genus Paradossenus F.O. longipes are included and a new species is described from Mato Grosso do Sul, Brazil and preliminary data on its biology are presented.
Abstract: Three Brazilian species of the genus Paradossenus F.O. Pickard-Cambridge 1903 are included in this paper: Paradossenus minimus (Mello-Leitao 1940), whose holotype was located and is here redescribed; Paradossenus corumba new species is described from Mato Grosso do Sul, Brazil and preliminary data on its biology are presented. Morphological data and new records of P. longipes (Tac- zanowski 1874) are included.

01 Jan 2000
TL;DR: Os animais criados em boas condicoes nutricionais e sanitarias possuem capacidade de superar a infeccao causada pelo isolado de T. vivax testado; no entanto, e recomendado nao substima-lo como agente secundario quando em associacao com outras patologias.
Abstract: SUMARIO: Realizou-se infeccao experimental em bovinos com Trypanosoma vivax, utilizando um isolado colhido na regiao sul do Pantanal, Estado de Mato Grosso do Sul, Brasil. Foram utilizados dois caprinos esplenectomizados para multiplicacao do inoculo, e inoculados cinco bovinos jovens, com 2,5x 10 5 Trypanosoma vivax por animal, mantidos em baias de isolamento por todo o periodo experimental. Os animais eram observados diariamente quanto a ingestao de alimentos e estado geral, periodicamente realizouse exames clinicos, registro da temperatura corporal, coleta de sangue para exames parasitologicos, hematologico, dosagens de fibrinogenio e proteinas. Ao final de sessenta e cinco dias foi realizado sacrificio e necropsia dos animais, seguido de exame anatomopatologico e histopatologico. Os animais mantiveram bom estado corporal, a ingestao de alimentos foi inalterada, a temperatura corporal media elevou-se nos dias 4 ao 9 mantendo-se em 39°C ate o dia 23 pos-infeccao (PI), quando retornou aos valores normais. O volume globular manteve-se dentro dos parâmetros previstos para a categoria animal, porem com acentuada reducao do dia 1 ao 18 PI elevando-se posteriormente. Foi observada leucocitose, com predominância de linfocitos a partir do dia 28 PI ate o final do experimento e eosinopenia entre os dias 23 e 60 PI. Detectou-se parasitas no sangue no dia 7 ate o 37, sendo que no dia 14 PI foi observada a maior quantidade. Apos o dia 57 PI nao foram detectadas formas parasitas no sangue. Nao ocorreram alteracoes nas dosagens de proteinas. Nao foram observadas alteracoes anatomopatologicas de caracter especifico. Em todos os animais constatou-se miocardite nao supurativa multifocal e em tres glomerulonefrite nao supurativa multifocal subaguda. Concluiu-se, frente aos resultados, que os animais criados em boas condicoes nutricionais e sanitarias possuem capacidade de superar a infeccao causada pelo isolado de T. vivax testado; no entanto, e recomendado nao substima-lo como agente secundario quando em associacao com outras patologias. PALAVRAS CHAVES: Trypanosoma vivax, tripanosomiase, bovinos, inoculacao experimental.


Journal ArticleDOI
01 Jan 2000
TL;DR: In this article, the authors analyzed the sensory acceptability of yacon flour (YF) added-cookies, and determined the chemical composition of the traditional formulation and of those containing the highest contents of YF with the sensory acceptance similar to the standard product.
Abstract: This study analyzed the sensory acceptability of yacon flour (YF) added-cookies, and determined the chemical composition of the traditional formulation and of those containing the highest contents of YF with the sensory acceptance similar to the standard product. Five formulations of biscuits have been prepared, being: standard (0 %) and four samples containing 5 %, 7.5 %, 10 % and 12.5 % of YF, respectively. For conducting the sensory analysis, 55 untrained tasters participated, who were diabetic patients of both genders, aged from 18 to 70 years. The results of sensory analysis showed that the addition of 7.5 % of YF was the best contents of yacon, and with the acceptance similar to the standard product, and mainly for the attributes appearance, aroma, taste, overall acceptance and purchase intention. The chemical analysis of standard samples and of 7.5 % YF-containing cookies showed higher levels of fiber, protein, ash and moisture in the sample with YF; however lower concentrations of carbohydrates and total calories were found. The development of products has demonstrated that the cookies containing YF up to 7.5 % were well accepted by the tasters and these samples evidenced the sensory acceptance similar to the standard product.

Journal ArticleDOI
TL;DR: In this article, the authors investigate the desenvolvimento dos principalios de justica distributiva, utilizado by criancas e adolescentes, em situacao hipotetica de distribuicao de recompensa and compara a evolucao do conceito of justic distributiva with as nocoes de certo e errado emitidas by esses mesmos participantes sobre perguntas a respeito de normas sociais escolares.
Abstract: Este estudo investiga o desenvolvimento dos principios de justica distributiva, utilizado por criancas e adolescentes, em situacao hipotetica de distribuicao de recompensa e compara a evolucao do conceito de justica distributiva com as nocoes de certo e errado emitidas por esses mesmos participantes sobre perguntas a respeito de normas sociais escolares. Examina as respostas que 90 criancas de terceira, quinta e setima series do Ensino Fundamental, de uma escola publica de Tres Lagoas - MS, deram a perguntas e historias-estimulo referentes as normas sociais escolares e ao conceito de justica distributiva com algumas reflexoes. Os resultados encontrados mostram que a crianca primeiramente responde as questoes guiada pelos resultados aparentes dos atos ou pela sua conformidade as regras e, posteriormente, avanca para a analise das intencoes; e observou-se um desenvolvimento progressivo da autonomia na passagem gradativa do principio de igualdade para o principio de equidade. Conclui-se que ha evolucao na utilizacao dos principios de justica distributiva, em diferentes idades e em cada sexo e que esta evolucao e acompanhada da evolucao de conceitos de certo e errado relacionados as normas sociais escolares.

Journal ArticleDOI
TL;DR: In this article, the seasonal changes of blood electrolytes concentration (calcium, phosphates, magnesium, potassium, chlorine, copper, and zinc) were evaluated using 20 graded Nelore cows on continuous grazing of Brachiaria decumbens.
Abstract: The seasonal changes of blood electrolytes concentration (calcium, phosphates, magnesium, potassium, chlorine, copper, and zinc) were evaluated using 20 graded Nelore cows on continuous grazing of Brachiaria decumbens. The cows were hypocalcemic and hypophosphatemic throughout the year. In the spring, during the pasture sprouting (November and December), cows grazing on clay soil were more predisposed to metabolic alkalosis, compared to cows kept on pastures located in sandy soil. Metabolic alkalosis was characterized by low blood levels of potassium and chlorine. Normal serum levels of copper, 0.79 and 9.81m g/ml, and zinc, 1.42 and 1.33m g/ml were observed in cows grazing on sandy and clay soils, respectively. However, these minerals were found in low concentrations on the pasture. The type of soil affected the mineral composition of the brachiaria pasture, and the blood serum levels of phosphates, potassium, magnesium, chlorine, copper, and zinc. Higher serum levels of phosphates, magnesium, potassium and chlorine were observed on animals grazing on sandy than on clay soil.

Book ChapterDOI
15 Jun 2000
TL;DR: The algorithm for determining whether a bipartite graph is convex includes a novel, coarse grained parallel, version of the PQ tree data structure introduced by Booth and Lueker, which solves the problem of testing the consecutive-ones property for (0, 1) matrices as well as the chordal graph recognition problem.
Abstract: In this paper, we present parallel algorithms for the coarse grained multicomputer (CGM) and the bulk synchronous parallel computer (BSP) for solving two well known graph problems: (1) determining whether a graph G is bipartite, and (2) determining whether a bipartite graph G is convex Our algorithms require O(log p) and O(log2 p) communication rounds, respectively, and linear sequential work per round on a CGM with p processors and N/p local memory per processor, N=|G| The algorithms assume that N/p ≥ pƐ for some fixed Ɛ > 0, which is true for all commercially available multiprocessors Our results imply BSP algorithms with O(log p) and O(log2 p) supersteps, respectively, O(g log(p)N/p) communication time, and O(log(p)N/p) local computation time Our algorithm for determining whether a bipartite graph is convex includes a novel, coarse grained parallel, version of the PQ tree data structure introduced by Booth and Lueker Hence, our algorithm also solves, with the same time complexity as indicated above, the problem of testing the consecutive-ones property for (0, 1) matrices as well as the chordal graph recognition problem These, in turn, have numerous applications in graph theory, DNA sequence assembly, database theory, and other areas