Jessore University of Science & Technology

About: Jessore University of Science & Technology is a based out in . It is known for research contribution in the topics: Adsorption & Population. The organization has 811 authors who have published 974 publications receiving 10471 citations.

In vitro plant regeneration of potato (Solanum tuberosum L.) at the rate of different hormonal concentration

Abstract: One of the goals of the experiment is to standardization of HgCl 2 treatment for explants sterilization. The objectives also include developing a reproducible cost effective protocol for large scale production of Solanum tuberosum of Cardinal variety plantlets from selectively better clones through plant in vitro propagation methods. Selection of growth regulators for proper multiple shoots regeneration, elongation and root induction. To produce genetically uniform plantlets within a short time capable surviving in natural condition raised in in vitro environment. Shoot tip and nodal segment explants from field grown plants were used as experimental materials in this investigation. All explants were cultured on Murashige and Skoog medium supplemented with various plant growth regulators. For surface sterilization of explants, HgCl 2 (0.1%) for 2 minutes was found to be most effective for complete destroying of surface pathogens and getting healthy tissues. Shoot regeneration was observed from both shoot tips and nodal explants for the studied plant. Maximum number of shoot per culture (17) was recorded and it also obtained the highest average length of the shoot (5cm) in Murashige and Skoog medium containing no hormone. On the other hand 6-benzyl amino purine (0.2mg/l) in 3 media showed the highest rate of shoot multiplication (73%) and the highest average length (4cm). In case of Gibberellic acid (0.1mg/l) in Murashige and Skoog media showed its highest rate of shoot regeneration (82%) and the highest average length (4.5cm). From the overall experiment it was observed that shoot tips are more responsive for micro propagation. In root induction Murashige and Skoog medium supplemented with different concentration (0.5, 1, 1.5 and 2mg/l) of indol-3-acetic acid and kinetin. Indol-3-acetic acid and kinetin (1.5+1.5 mg/l) showed its lowest rate of root regeneration (40%) and the average length of the root (1.5 cm). On the contrary Murashige and Skoog medium with no hormone showed the rate of root regeneration (96%) and the highest average length of the root (2.5 cm). The supplemented Murashige and Skoog media with no hormone showed the best performance for root regeneration. Asian J. Med. Biol. Res. June 2015, 1(2): 297-303

MERISTIC AND MORPHOMETRIC VARIATIONS OF BARRED SPINY EEL Macrognathus pancalus POPULATIONS FROM BANGLADESHI FRESHWATERS: AN INSIGHT INTO LANDMARK-BASED TRUSS NETWORK SYSTEM

Abstract: The present research focuses to examine the meristic and landmark-based morphometric variations of barred spiny eel Macrognathus pancalus from four populations namely Dhakuria beel, Jessore (DBJ); Bohnni boar, Gopalgonj (BBG); the Arial kha river, Madaripur (AKRM) and the Nabaganga river, Jhenidah (NRJ) in Bangladeshi freshwaters. Six meristic counts were compared among four populations and significant differences were observed in number of caudal fin rays and number of pelvic fin rays in all meristic characters. The truss protocol was used in the present study based on six general morphometrics and fifteen truss measurements. Univariate statistics showed that five (standard length (SL), upper jaw length (UJL), lower jaw length (LJL), head depth (HD) and inter orbital (IO)) of six morphometric and eleven truss networks (viz. 2-3, 3-4, 5-6, 6- 7, 7-8, 1-8, 2-8, 3-8, 3-7, 4-7 and 5-7) among fifteen truss measurements differed significantly. Cannonical discriminate function analyses were conducted among samples and the populations were fully intermingled. Pooled within group correlation showed morphometric and landmark measurements; the first discriminant function (DF) accounted for 79%, the second DF accounted for 15.9% and third DF accounted for 5.1% of group variability. An euclidian dendrogram was prepared based on morphometric and truss measurements in four populations, where two clusters were mainly formed, in which first cluster formed by NRJ population was fully separated, and the second cluster was formed by two populations of AKRM and DBJ. Additionally, a subcluster BBG was formed with AKRM. On the basis of morphometric and truss measurements, 68.8%, 83.6%, 55.6% and 80% of original grouped cases were correctly classified in NRJ, AKRM, DBJ and BBG, respectively. These results specify the presence of different stocks of fish from four aquatic habitats. This study is highly significant for proper conservation and effective management of M. pancalus populations as well as for the scientific community for further research.