Institution
National Health and Family Planning Commission
Government•Beijing, China•
About: National Health and Family Planning Commission is a government organization based out in Beijing, China. It is known for research contribution in the topics: Population & Kashin–Beck disease. The organization has 2379 authors who have published 1440 publications receiving 20078 citations. The organization is also known as: Ministry of Health of the People's Republic of China.
Topics: Population, Kashin–Beck disease, Medicine, Pregnancy, Health care
Papers
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TL;DR: This study lastly engineered SlugCas9 with mutations to generate a high fidelity variant that maintains high specificity without compromising on-target editing efficiency, and generated a Slug Cas9-SaCas9 chimeric nuclease, which has both high specificity and high activity.
Abstract: The compact CRISPR/Cas9 system, which can be delivered by adeno-associated virus (AAV), is a promising platform for therapeutic applications. However, current compact Cas9 nucleases have limited activity, targeting scope and specificity. Here, we identified three compact SaCas9 orthologs, Staphylococcus lugdunensis Cas9 (SlugCas9), Staphylococcus lutrae Cas9 (SlutrCas9) and Staphylococcus haemolyticus Cas9 (ShaCas9), for mammalian genome editing. Interestingly, SlugCas9 recognizes a simple NNGG PAM and displays comparable activity to SaCas9. We further generated a SlugCas9-SaCas9 chimeric nuclease, which has both high specificity and high activity. We lastly engineered SlugCas9 with mutations to generate a high fidelity variant that maintains high specificity without compromising on-target editing efficiency. Our study offers important minimal Cas9 tools that are ideal for both basic research and clinical applications.
15 citations
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TL;DR: A new steroid glucoside, along with nine known steroids and four known sorbicillinoids, were isolated from the endophytic fungus Trichoderma sp.
15 citations
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TL;DR: It is shown that rat morphine self-administration (MSA), a paradigm that effectively mimics human opiate addiction, increases NSC neuronal differentiation and promotes neuronal dendrite growth in the adult DG, and that targeting MOR selectively in the DG NSC population might offer a novel therapeutic intervention for morphine addiction.
Abstract: Adult hippocampal dentate gyrus (DG) neural stem cells (NSCs) continuously undergo proliferation and differentiation, producing new functional neurons that remodel existing synaptic circuits. Although proliferation of these adult DG NSCs has been implicated in opiate dependence, whether NSC neuronal differentiation and subsequent dendritogenesis are also involved in such addictive behavior remains unknown. Here, we ask whether opiate exposure alters differentiation and dendritogenesis of DG NSCs and investigate the possibility that these alterations contribute to opiate addiction. We show that rat morphine self-administration (MSA), a paradigm that effectively mimics human opiate addiction, increases NSC neuronal differentiation and promotes neuronal dendrite growth in the adult DG. Further, we demonstrate that the μ-opioid receptor (MOR) is expressed on DG NSCs and that MSA leads to a two-fold elevation of endogenous MOR levels in doublecortin expressing (DCX+) NSC progenies in the rat DG. MOR expression is also detected in the cultured rat NSCs and morphine treatment in vitro increases NSC neuronal differentiation and dendritogenesis, suggesting that MOR mediates the effect of morphine on NSC neuronal differentiation and maturation. Finally, we show that conditional overexpression of MOR in DG NSCs under a doxycycline inducible system leads to facilitation of the acquisition of MSA in rats, without affecting the extinction process. We advocate that targeting MOR selectively in the DG NSC population might offer a novel therapeutic intervention for morphine addiction.
15 citations
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TL;DR: Health economics and outcomes research (HEOR) will be instrumental to evidence-based decision making and is expected to play a major role in China’s health care reform.
Abstract: The health care system in China has been undergoing reforms for the past three decades. New policies and regulations are being developed to better meet the health needs of over a billion people. As reform programs expand, the need for scientific evidence increases, to enable accurate evaluation of the impact of new policies. In this context, health economics and outcomes research (HEOR) will be instrumental to evidence-based decision making and is expected to play a major role in China’s health care reform.
15 citations
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TL;DR: This research presents a novel probabilistic approach to characterize the immune checkpoints of viral infection and its role in pathogenic and non-pathogenic infections.
Abstract: 1CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; 2Division of Major Special Projects, Department of Health Science, Technology and Education at China’s National Health and Family Planning Commission, Beijing 100044, China; 3National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
15 citations
Authors
Showing all 2403 results
Name | H-index | Papers | Citations |
---|---|---|---|
Feng Zhang | 172 | 1278 | 181865 |
Yang Yang | 171 | 2644 | 153049 |
Lei Zhang | 135 | 2240 | 99365 |
Jian Zhang | 107 | 3064 | 69715 |
Wei Wang | 95 | 3544 | 59660 |
Jie Li | 76 | 843 | 32221 |
Jing Liu | 73 | 1351 | 27169 |
Haidong Kan | 71 | 405 | 44210 |
Wei Wang | 66 | 673 | 20023 |
Jin-Tai Yu | 66 | 439 | 20020 |
Qi Jin | 64 | 335 | 45892 |
Chuan Qin | 60 | 326 | 21708 |
Ji-Sheng Han | 60 | 339 | 13660 |
Ying Zhou | 60 | 663 | 14349 |
Jun Huang | 57 | 445 | 12176 |