National Health and Family Planning Commission

About: National Health and Family Planning Commission is a government organization based out in Beijing, China. It is known for research contribution in the topics: Population & Kashin–Beck disease. The organization has 2379 authors who have published 1440 publications receiving 20078 citations. The organization is also known as: Ministry of Health of the People's Republic of China.

Outcomes of total knee arthroplasty in the adult Kashin-Beck disease with severe osteoarthritis

Abstract: Kashin-Beck disease (KBD) is an endemic osteoarthropathy, and the severe knee pain and functional limitations were seriously affecting the quality of life in patients with end-stage KBD. We retrospectively evaluated the clinical outcomes and the quality of life in KBD patients with total knee arthroplasty (TKA). A total of 22 subjects (25 knees) suffered KBD with severe knee pain and underwent primary TKA. Knee pain was measured by visual analogue scale (VAS), and the knee function was evaluated by Knee Society Clinical Rating System Score (KSS). KBD Quality of Life (KBDQOL) was used to evaluate the quality of life in KBD patients before and after TKA. There were no major complications after TKA. The levels of VAS score were obviously deceased in post-operation than that in pre-operation. The levels of KSS score were increased in one year after TKA compared with the pre-operative values, and it maintained a higher level on three years after TKA. The average KBDQOL score level of each domain in pre-operation and one and three years after TKA was increased accordingly. The average scores of physical function, activity limitation, support of society, mental health, and general health in one year after TKA were significantly higher than those in pre-operation. TKA can reduce knee pain, improve knee function, and improve the quality life in KBD patients. KBDQOL questionnaire may be a promising instrument for assessing the quality life in KBD patients.

Integrating genome-wide association study with regulatory SNP annotation information identified candidate genes and pathways for schizophrenia.

Abstract: Background Schizophrenia is a complex mental disorder. The genetic mechanism of schizophrenia remains elusive now. Methods We conducted a large-scale integrative analysis of two genome-wide association studies of schizophrenia with functional annotation datasets of regulatory single-nucleotide polymorphism (rSNP). The significant SNPs identified by the two genome-wide association studies were first annotated to obtain schizophrenia associated rSNPs and their target genes and proteins, respectively. We then compared the integrative analysis results to identify the common rSNPs and their target regulatory genes and proteins, shared by the two genome-wide association studies of schizophrenia. Finally, DAVID tool was used to conduct gene ontology and pathway enrichment analysis of the identified targets genes and proteins. Results We detected 53 schizophrenia-associated target genes for rSNP, such as FOS (P value = 2.18×10-20), ATXN1 (P value = 5.22×10-21) and HLA-DQA1 (P value = 1.98×10-10). Pathway enrichment analysis identified 24 pathways for transcription factors binding regions, chromatin interacting regions, long non-coding RNAs, topologically associated domains, circular RNAs and post-translational modifications, such as hsa05034:Alcoholism (P value = 2.57×10-7) and hsa04612:Antigen processing and presentation (P value = 6.82×10-8). Conclusion We detected multiple candidate genes, gene ontology terms and pathways for schizophrenia, supporting the functional importance of rSNPs, and providing novel clues for understanding the genetic architecture of schizophrenia.

Integrative analysis of transcriptome-wide association study data and messenger RNA expression profiles identified candidate genes and pathways for inflammatory bowel disease.

Abstract: Inflammatory bowel disease (IBD) is a complex disease, resulting from abnormal immune response to intestinal tract microbiota in genetically susceptible individuals. Crohn's disease and ulcerative colitis (UC) are the two major types of IBD. Transcriptome-wide association study (TWAS) of IBD was first performed using a large-scale genome-wide association study summary data sets of IBD. The FUSION software was applied for TWAS, considering various tissues and cells. The genes identified by TWAS were then validated by the gene expression profiling data sets of IBD. The functional annotation and potential pathways of common differentially expressed genes were further subjected to gene ontology (GO) and pathway enrichment analysis. Integrative analysis of TWAS and messenger RNA (mRNA) expression data detected several tissues related common genes for UC, such as HLA-DRB1 (PTWAS = 0.024; mRNA expression ratio = 1.700) and TAP2 in colon (P TWAS = 0.047; mRNA expression ratio = 2.170). Further comparing the GO enrichment analysis results of TWAS and mRNA expression data, we identified 11 common GO terms for UC, such as plasma membrane (P value = 5.08 × 10-10 ) in intestinal tissues and immune response (P = 0.001) in peripheral blood. We also detected several common pathways for UC, including cell adhesion molecules (P = 0.003) in intestinal tissues, IBD (P = 0.049) in whole blood and phagosome (P = 0.0003) in peripheral blood. Our study results provide novel clues for understanding the genetic mechanism of IBD.

LRP6 Knockdown Ameliorates Insulin Resistance via Modulation of Autophagy by Regulating GSK3β Signaling in Human LO2 Hepatocytes.

Abstract: Recent studies suggest that autophagy is highly involved in insulin resistance (IR). Inhibition of the PI3K/AKT/mTOR signaling pathway induces autophagy activation. Additionally, depletion of LRP6 has been shown to increase insulin sensitivity but its mechanism is still not clear. We hypothesized that LRP6 contributes to IR by regulating mTOR mediated autophagy through GSK3β in hepatocytes. LO2 hepatocytes were treated with palmitate (PA) and insulin to induced IR. Levels of LRP6 mRNA and protein expression were measured by real time-PCR and western blot analysis. LRP6 knock down was achieved by adenovirus mediated Si-LRP6 expression and its roles in IR, glucose, GSK3β, mTOR signaling, and autophagy were explored. Finally, GSK3β was overexpressed and its involvement in autophagy and IR was examined. We found that PA treatment led to a reduced glucose uptake and IR in hepatocytes, which was accompanied by an upregulation of LRP6 expression. Knocking down of LRP6 enhanced glucose uptake and insulin sensitivity in PA treated cells, probably through increasing GSK3b activity. Overexpression of GSK3b mimicked LRP6 reduction by enhancing autophagy and ameliorating IR. Our study revealed a significant molecular mechanism connecting LRP6 to insulin sensitivity through GSK3β-mTOR mediated autophagy.

The status of postpartum weight retention and its associated factors among Chinese lactating women in 2013

Abstract: Objective: To analyze the status of postpartum weight retention and its associated factors among Chinese lactating women in 2013. Methods: This study was based on the databank of Chinese National Nutrition and Health Surveillance in 2013. Using the multi-stage stratified cluster sampling method, we enrolled 12 514 women, at 0-24 months postpartum, from 55 sites in 30 provinces of China (excluding the Tibet Autonomous Region). Questionnaires were used to collect data on basic characteristics, physical activity, lifestyle, food intake, pre-pregnancy weight, weight before delivery, disease history during pregnancy, delivery date, delivery mode, parity and breastfeeding information. Current body weight and height of each subject were measured. Postpartum weight retention in each subject was calculated as the difference between pre-pregnancy and current weights. High postpartum weight retention was defined as ≥5 kg. Survey sample weights were calculated according to the sampling design and number of children under 2 years old from 2010 population census data of China. Associated factors of high postpartum weight retention were identified by multiple logistic regression analysis. Results: A total of 9 972 women were included in this study. P50 (P25-P75) of weight retention for participants was 3.6 (0.2-7.3) kg and high weight retention accounted for 41.5% of the subjects (4 134/9 972). The weighted means (SE) of weight retention was 3.5 (0.1) kg, and it was at 0 to <3, 3 to <6, 6 to <9, 9 to <12, 12 to <18 and 18 to <24 months postpartum were 5.3 (0.2), 4.5 (0.2), 3.8 (0.2), 3.1 (0.2), 2.8 (0.2) and 3.0 (0.2) kg, respectively. The weighted proportion of high postpartum weight retention was 37.4% (95%CI: 36.0%-38.9%). Compared with women aged under 25 years old, the odds ratio of high postpartum weight retention was 1.31 for women over 30 years old. Compared with women at 18 to <24 months postpartum, the odds ratio of high postpartum weight retention was 2.67, 1.61 and 1.31 for women at 0 to <3, 3 to <6 and 6 to <9 months postpartum, respectively. Compared with women having a pre-pregnant BMI from 18.5 to <25.0 kg/m2, the odds ratio of high postpartum weight retention was 1.30 and 0.67 for women having pre-pregnant BMI <18.5 kg/m2 and ≥25.0 kg/m2, respectively. Compared with women having appropriate gestational weight gain, the odds ratio of high postpartum weight retention was 0.47 and 2.24 for women having low and excessive gestational weight gains, respectively. Compared with farmers, the odds ratio of high postpartum weight retention was 1.44 for housewives. (P<0.05 for all comparisons). Conclusion: Postpartum weight retention in Chinese lactating women is at an intermediate level. High postpartum weight retention is primarily associated with age, postpartum period, pre-pregnant BMI, gestational weight gain and career. The key control factor for weight retention is gestational weight gain, and 1 year postpartum is critical period for weight recovery.