Renji Hospital

About: Renji Hospital is a healthcare organization based out in Shanghai, China. It is known for research contribution in the topics: Medicine & Biology. The organization has 1112 authors who have published 714 publications receiving 15442 citations. The organization is also known as: Rénjì Yīyuàn.

Effects of carbon‐to‐nitrogen ratio (C:N) on water quality and growth performance of <i>Litopenaeus vannamei</i> (Boone, 1931) in the biofloc system with a salinity of 5‰

Abstract: This study aimed to investigate the effects of carbon-to-nitrogen ratio (C:N) on water quality and shrimp growth performance during the grow-out culture of Litopenaeus vannamei in a biofloc system under a low salinity condition. Three biofloc treatments with a C:N (contained in feed and exogenous carbon source with the assumption that 75% of the feed nitrogen is excreted) of 8:1 (CN8), 16:1 (CN16) and 24:1 (CN24), respectively, were designed to stocking shrimp juveniles (~0.8 g shrimp−1) at a density of 270 individuals m−3, for a 63-day culture experiment at a salinity of about 5‰. Water parameters were monitored, and zootechnical indices were determined in the three treatments. Results showed that in CN8 treatment, pH, carbonate alkalinity, biofloc volume and TSS were significantly lower (p < 0.05), whereas TAN, nitrite and nitrate were significantly higher, than those in the other two treatments (p < 0.05). The zootechnical parameters of shrimp were not significantly different among the three treatments (p > 0.05), except that the survival rates in CN16 treatment (96.8 ± 2.0%) and CN24 treatment (93.7 ± 4.2%) were significantly higher than that of CN8 treatment (81.5 ± 6.4%, p < 0.05). Regression analysis revealed that the optimal C:N range for pH, SVI (sludge volume index), TAN, nitrate and survival rate was 20.5:1, 18.5:1, 21.0:1, 20.8:1 and 18.6:1. The results suggested that the C:N higher than 16 were suitable for culturing L. vannamei in the biofloc system with a salinity of 5‰, with an optimal range of 18.5–21.0:1.

Research of ALA combined with HpD-PDT which induced S180 ascitic tumor cells, death, or apoptosis on cytology

Abstract: Objective: To ascertain the adequate dosage of ALA combined with HpD-PDT which induced tumor cell death or apoptosis on cytology. And to study the different effect of ALA-PDT and HPD-PDT used only. Methods: Rat ascitic tumor cells(S180) were randomly divided into several groups and incubated with ALA (20μg/ml, 40μg/ml, 80μg/ml, 160μg/ml), HPD (2.5μg/ml, 5μg/ml, 10μg/ml) and their combination dosages. 630nm light (total output 2W) was delivered to tumor cells at a constant fluence rate: 200mw/cm 2 and a constant irradiated time period: 20 minutes. We set 3 groups (no photosensitizers or no irradiation or neither) to be the control groups. We used inversion microscopy to observe the morphological change of tumor cells and flow cytometry technology to detect the death or apoptosis of tumor cells during the experiment. Results: After irradiated with 630nm light on 20 minutes, S180 tumor cells incubated with the combination dosage of ALA 40μg/ml and HPD2.5μg/ml were induced highest rate of apoptosis. The rate of cells' early apoptosis was 2.54%, while the late apoptosis was 95.10% Conclusion: The combination dosage of ALA 40μg/ml and HPD2.5μg/ml (25% of constant dosage) used in the PDT treatment was the most adequate dosage on cytology.

[The subpopulations of T lymphocyte and the function of suppressor T cell in normal pregnancy and pregnancy-induced hypertension].

Abstract: T-lymphocyte subpopulations were determined by the monoclonal antibodies (OKT3, OKT4, OKT8) in 20 cases of normal pregnancy, 36 cases of pregnancy-induced hypertension (PIH) and 20 cases of normal non-pregnant women. The assaying of ConA-induced suppressor T cell (Ts cell) function was carried out in another 20 cases of late pregnancy, 20 cases of moderate and severe PIH, and 10 cases of normal non-pregnant women. The results showed: the percentage of Ts cell increased significantly and the ratios of Th/Ts decreased significantly during normal pregnancy as compared with normal non-pregnant women. As compared with the late pregnant women, a lowering of Ts cell and elevating ratio of Th/Ts were found in PIH cases with statistical significance in severe PIH. A decrease of Ts cell function was also seen in moderate and severe PIH cases. All these findings suggest that the changes of Ts cell in amount and function may play an important role in maintaining normal pregnancy and in the pathogenesis of PIH.

TGF-β1 signaling remodels TH17 cell chromatin architecture for metabolic reprogramming

Abstract: TH17 cells exhibit great heterogeneity and variable functional states in vivo. However, metabolic reprogramming of TH17 cells in vivo and its regulation during autoimmunity and host defence is unknown. Here we report that TH17 cells derived in vivo show discrete metabolic states. Metabolic states of TH17 cells in vivo were controlled at the epigenetic level, with conserved regulatory region of key metabolic regulators show distinct chromatin accessibility as demonstrated by chromatin landscape profiling. TGF-β1 signaling was further shown to be crucial for remodeling of TH17 cell chromatin states, Ahr and miR-21 were identified as essential metabolic regulators for TH17 cells. Understanding metabolic reprogramming of TH17 cells in vivo may therefore provide more defined therapeutic intervention to TH17 cell mediated autoimmune diseases and insights into TH17 cell mediated host defence.

Effect of Helicobacter pylori infection on cyclooxygenase-2 expression in gastric antral mucosa

Abstract: OBJECTIVE: Helicobacter pylori infection is a major etiological cause of chronic gastritis. Inducible cyclooxygenase (COX-2) is an important regulator of mucosal inflammation. Recent studies indicate that expression of COX-2 may contribute to gastro­intestinal carcinogenesis. The aim of this study was to investigate the effects of H. pylori infection and eradication therapy on COX-2 expression in gastric antral mucosa. METHODS: Antral biopsies were taken from 46 H. pylori-infected patients, who also had chronic gastritis, both before and after anti-H. pylori treatment. The COX-2 protein was stained by using immunohistochemical methods and COX-2 expression was quantified as the percentage of epithelial cells expressing COX-2. Gastritis and H. pylori infection status were graded according to the Sydney system. RESULTS: Cyclooxygenase-2 expression was detected in the cytoplasm of gastric antral epithelial cells both before and after the eradication of H. pylori. Cyclooxygenase-2 expression in mucosa with H. pylori infection was compared with the corresponding mucosa after successful H. pylori eradication (20.1 ± 13.1%vs 13.8 ± 5.9%; P < 0.05). At the same time, COX-2 expression in H. pylori-infected mucosa was com­pared with the normal controls (18.0 ± 14.1%vs 12.3 ± 4.6%, P < 0.05). Expression of COX-2 was correlated with the degree of chronic inflammation (r= 0.78, P < 0.05). CONCLUSIONS: Our results showed that H. pylori infection leads to gastric mucosal overexpression of COX-2 protein, suggesting that the enzyme is involved in H. pylori-related gastric pathology in humans.